AB284620

Human Reelin ELISA Kit

Recombinant
SimpleStep
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Human Reelin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Reelin with a sensitivity of 0.115 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Reelin, RELN

4 Images

Sandwich ELISA - Human Reelin ELISA Kit (AB284620)

sELISA

Supplier Data

Sandwich ELISA - Human Reelin ELISA Kit (AB284620)

Example of human Reelin standard curve in 1X Cell Extraction Buffer PTR.

Example of human Reelin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Reelin ELISA Kit (AB284620)

Interpolated concentrations of human Reelin in serum, plasma (citrate), plasma (EDTA), and plasma (heparin).

Interpolated concentration of native Reelin was measured in duplicate at different sample concentrations. Undiluted samples are 125 ug/mL HepG2 extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human Reelin ELISA Kit (AB284620)

Interpolated concentrations of human Reelin in serum, plasma (citrate), plasma (EDTA), and plasma (heparin).

Interpolated concentration of native Reelin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 100%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Reelin ELISA Kit (AB284620)

Example of human Reelin standard curve in Sample Diluent NS.

Example of human Reelin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Serum, Cell Lysate, EDTA Plasma, Citrate plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.115 ng/mL

Range

1.09 - 70 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Reelin ELISA kit (ab284620) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Reelin protein in human serum, plasma, and cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Reelin with 0.115 ng/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" } ]

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Recovery

[ { "sample": "Serum", "range": "102 - 111 %", "average": "= 105" }, { "sample": "EDTA Plasma", "range": "105 - 112 %", "average": "= 109" }, { "sample": "Heparin Plasma", "range": "89 - 97 %", "average": "= 94" }, { "sample": "Cell Lysate", "range": "80 - 92 %", "average": "= 86" }, { "sample": "Citrate plasma", "range": "99 - 115 %", "average": "= 105" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Reelin a large glycoprotein with a mass of approximately 388 kDa plays an integral role in brain development and function. Known for its presence in the extracellular matrix it is frequently referred to as RELN protein. Reelin is predominantly expressed in the brain especially in regions such as the cerebral cortex hippocampus and cerebellum. Its expression is highly regulated suggesting its importance in neurological processes.

Biological function summary

Reelin functions in the regulation of neuronal migration and positioning in the developing brain. Acting as a signaling molecule it forms part of a larger complex essential for proper neuronal layering and synaptic plasticity. Reelin interacts with various receptors on the cell surface initiating a cascade of intracellular signaling that influences cell orientation and dendritic growth.

Pathways

Reelin engages both the Reelin signaling pathway and the PI3K-Akt pathway highlighting its diverse influence on cellular processes. In the Reelin signaling pathway it associates with proteins like Dab1 and VLDLR playing an important role in the modulation of tyrosine kinases. This association affects neuronal structures by guiding their architectural development while interactions with the PI3K-Akt pathway impact cell survival and growth.

Reelin has been implicated in conditions such as lissencephaly and autism spectrum disorders. Altered Reelin signaling may contribute to the abnormal neuronal migration seen in these diseases. Moreover its interplay with other proteins like ApoE which modifies brain structure and function in these disorders highlights Reelin’s relevance in maintaining neurological health.

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Product protocols

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Target data

Extracellular matrix serine protease secreted by pioneer neurons that plays a role in layering of neurons in the cerebral cortex and cerebellum by coordinating cell positioning during neurodevelopment. Regulates microtubule function in neurons and neuronal migration. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion.

See full target information RELN

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Reelin ELISA Kit

Sandwich ELISA - Human Reelin ELISA Kit (AB284620)

Sandwich ELISA - Human Reelin ELISA Kit (AB284620)

Sandwich ELISA - Human Reelin ELISA Kit (AB284620)

Sandwich ELISA - Human Reelin ELISA Kit (AB284620)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Product promise