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AB314377

Human REG1B ELISA Kit

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Human REG1B ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human REG1B in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

PSPS2, REGL, REG1B, Lithostathine-1-beta, Pancreatic stone protein 2, Regenerating islet-derived protein 1-beta, Regenerating protein I beta, PSP-2, REG-1-beta

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Sandwich ELISA - Human REG1B ELISA Kit (AB314377)
  • sELISA

Supplier Data

Sandwich ELISA - Human REG1B ELISA Kit (AB314377)

Example of standard curve using ab314377. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 20 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human REG1B ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human REG1B in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human REG1B coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human AMIGO, Aminopeptidase LRAP (ERAP2), Amnionless, Arylsulfatase A, Bcl-w, CD109, CD157, CD34, CD83, CLEC-1, CLEC10A (CD301), CMG-2, CREG, Cystatin SN, Cytokeratin-8, Dectin-1 (CLEC7A), Desmocollin-3, Endoglycan, Galectin-4, HAPLN1, Jagged 1, Langerin (CD207), Lumican, Matriptase (ST14), MEP1B, Nectin-3, OX40 (TNFRSF4), OX40 Ligand (TNFSF4), p27, Pappalysin-2, Plexin B3, Plexin D1, proGRP, PSA-total, RGM-A, ROBO2, Spinesin, TWEAK R (TNFRSF12), ULBP-3.

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Recovery

[ { "sample": "Serum", "range": "78 - 102 %", "average": "= 88.46" }, { "sample": "Plasma", "range": "68 - 84 %", "average": "= 75.43" }, { "sample": "Cell culture media", "range": "127 - 147 %", "average": "= 137.8" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314377-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin", "number":"AB314377-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314377-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314377-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human REG1B Antibody-coated ELISA Plate", "number":"AB314377-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human REG1B Protein Standard", "number":"AB314377-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314377-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314377-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human REG1B Detection Antibody", "number":"AB314377-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

REG1B also known as Regenerating islet-derived protein 1B is a small secretory protein with a molecular mass of approximately 18 kDa. This protein is expressed mainly in the pancreas with some expression noted in other tissues such as the gastrointestinal tract. It plays a role in the secretion and regulation of various cellular processes. REG1B belongs to the Reg family of proteins which function as lectins that bind to specific carbohydrate molecules.
Biological function summary

REG1B involves itself in pancreatic repair and regeneration. Its involvement in cell proliferation suggests that it plays a part in tissue recovery. REG1B is not typically part of a larger complex but it may interact with other proteins to carry out its function. Its presence has been noted during the regenerative processes in acinar cells of the pancreas following injury or stress proposing its participation in tissue defense mechanisms.

Pathways

The role of REG1B becomes evident in processes relating to pancreatic health and diabetes. It plays a role in the inflammatory and cellular stress response pathways. REG1B can also interact with other family members like REG1A to coordinate response activities contributing to cell signaling and regeneration potentially through modulation of local inflammation and cellular growth signals.

REG1B associates closely with conditions involving pancreatic disorders such as pancreatitis and pancreatic cancer. The protein might stimulate or inhibit cellular pathways that can contribute to the development or progression of these conditions. REG1B links to inflammatory process related proteins such as cytokines and its expression levels may change during disease progression indicating a potential biomarker role.
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Product protocols

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Target data

Might act as an inhibitor of spontaneous calcium carbonate precipitation. May be associated with neuronal sprouting in brain, and with brain and pancreas regeneration.
See full target information REG1B
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB233210

Anti-REG1B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG1B antibody (AB233210)

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Proteins & Peptides

AB276414

Recombinant Human REG1B protein (His tag)

SDS-PAGE - Recombinant Human REG1B protein (His tag) (AB276414)

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