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AB267626

Human REG3A ELISA Kit

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Human REG3A ELISA Kit is a Sandwich ELISA for the measurement of Human REG3A in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

HIP, PAP, PAP1, REG3A, Regenerating islet-derived protein 3-alpha, REG-3-alpha, Hepatointestinal pancreatic protein, Human proislet peptide, Pancreatitis-associated protein 1, Regenerating islet-derived protein III-alpha, HIP/PAP, Reg III-alpha

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Sandwich ELISA - Human REG3A ELISA Kit (AB267626)
  • sELISA

Supplier Data

Sandwich ELISA - Human REG3A ELISA Kit (AB267626)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.12 ng/mL

Range

0.12 - 25 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human REG3A ELISA Kit is designed for the quantitative determination of REG3A in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for human REG3A coated on a 96-well plate. Standards and samples are pipetted into the wells and REG3A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human REG3A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of REG3A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit shows no cross-reactivity with the following cytokines tested: Human ADAM22, Arylsulfatase B/ARSB, B3GNT2, Carbonic Anhydrase VB, Caspase 7, Caspase 8, Integrin alpha M/CD11b, SIRP gamma/CD172g, CD39L2/ENTPD6, CD39L4/ENTPD5, Integrin alpha 2/CD49b, CD7, CEACAM3, Carboxypeptidase E/CPE, FABP6, FAM3C, GDF-3, GSTM1, Kallikrein 11, Kallikrein 12, Kremen-2, OSCAR, PTP1B, R-Spondin 2, S100A13, Semaphorin 4C, Sirtuin 1/SIRT1, SMPD1, Sortilin, SPINK1, Stabilin-2, SULT2A1, Transcobalamin II, THSD1, TrkA, UCH-L3, VAPA, vWF-A2, Wnt-4.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "117 - 138 %", "average": "= 128.5" }, { "sample": "Plasma", "range": "97 - 146 %", "average": "= 119.1" }, { "sample": "Cell culture media", "range": "109 - 140 %", "average": "= 121.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267626-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267626-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267626-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human REG3A coated Microplate (12 x 8 wells)", "number":"AB267626-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human REG3A Standard (Lyophilized)", "number":"AB267626-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267626-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB267626-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human REG3A Detection Antibody", "number":"AB267626-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB267626-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB267626-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Reg3a also known as regenerating islet-derived protein 3 alpha or PAP1 (pancreatitis-associated protein 1) is a C-type lectin with a molecular mass of approximately 19 kDa. This protein is primarily found in the epithelial cells of the intestinal tract where it plays a significant role in the mucosal immune response. Reg3a is secreted into the intestinal lumen indicating its importance in maintaining gut homeostasis and defending against pathogenic bacteria. Expression of Reg3a increases upon inflammatory stimuli suggesting a strong link between inflammation and its function.
Biological function summary

Reg3a acts as an antimicrobial peptide that targets bacterial cell walls specifically binding to peptidoglycans. This ability to bind and break down the cell wall helps in controlling bacterial populations within the gut therefore Reg3a becomes an innate defense component. It works independently and is not known to be part of a larger protein complex. Moreover Reg3a shows bactericidal activity against Gram-positive bacteria contributing to the maintenance of a healthy gut flora.

Pathways

Reg3a functions prominently in the innate immune response and inflammatory pathways. The protein gets upregulated in response to cytokines like Interleukin-22 (IL-22) which helps Reg3a to exert its antimicrobial properties. Reg3a shares relatedness with proteins like Reg3g when considering their roles in the protection of intestinal epithelial barriers. These proteins work together to safeguard the gut lining by destroying harmful bacterial invaders therefore playing an important role in maintaining intestinal integrity.

Reg3a has been implicated in inflammatory bowel diseases such as Crohn's disease and ulcerative colitis. Studies show altered expression levels of Reg3a in these disorders connecting its antimicrobial function with disease pathogenesis. Additionally Reg3a shows as a biomarker for pancreatic disorders such as chronic pancreatitis potentially due to changes in its expression during inflammatory episodes. Reg3a engages with inflammation-related proteins including Tumor Necrosis Factor-alpha (TNF-alpha) which may influence disease outcomes and progression.
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Product protocols

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Target data

Regenerating islet-derived protein 3-alpha 15 kDa form. Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan (PubMed : 16931762). Binds membrane phospholipids and kills bacteria by forming a hexameric membrane-permeabilizing oligomeric pore (PubMed : 24256734).. Acts as a hormone in response to different stimuli like anti-inflammatory signals, such as IL17A, or gut microbiome. Secreted by different cell types to activate its receptor EXTL3 and induce cell specific signaling pathways (PubMed : 19158046, PubMed : 22727489, PubMed : 27830702, PubMed : 34099862). Induced by IL17A in keratinocytes, regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway (PubMed : 22727489). In parallel, inhibits skin inflammation through the inhibition of inflammatory cytokines such as IL6 and TNF (PubMed : 27830702). In pancreas, is able to permealize beta-cells membrane and stimulate their proliferation (PubMed : 19158046).. Regenerating islet-derived protein 3-alpha 16.5 kDa form. Has bacteriostatic activity.
See full target information REG3A
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