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AB320799

Human REG4 ELISA Kit

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Human REG4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human REG4 with a sensitivity of 2.23 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

GISP, RELP, REG4, Regenerating islet-derived protein 4, REG-4, Gastrointestinal secretory protein, REG-like protein, Regenerating islet-derived protein IV, Reg IV

6 Images
Sandwich ELISA - Human REG4 ELISA Kit (AB320799)
  • sELISA

Supplier Data

Sandwich ELISA - Human REG4 ELISA Kit (AB320799)

Interpolated concentrations of human REG4 in PC-3 supernatant. Interpolated concentration of native REG4 was measured in duplicate at different sample concentrations. Undiluted samples are 100% PC-3 supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human REG4 ELISA Kit (AB320799)
  • sELISA

Supplier Data

Sandwich ELISA - Human REG4 ELISA Kit (AB320799)

Interpolated concentrations of human REG4 in serum in 96-well vs. 384-well plates. Interpolated concentration of native REG4 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50% serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human REG4 ELISA Kit (AB320799)
  • sELISA

Supplier Data

Sandwich ELISA - Human REG4 ELISA Kit (AB320799)

Interpolated concentrations of human REG4 in serum and plasma. Interpolated concentration of native REG4 was measured in duplicate at different sample concentrations. Undiluted samples are 25% serum, 50% plasma (citrate), 50% plasma (EDTA), and 50% plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human REG4 ELISA Kit (AB320799)
  • sELISA

Supplier Data

Sandwich ELISA - Human REG4 ELISA Kit (AB320799)

Interpolated concentrations of REG4 in normal human serum donors. Serum of eight individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean REG4 concentration was determined to be 1,533 pg/mL with a range of 673 – 3,280 pg/mL.

Sandwich ELISA - Human REG4 ELISA Kit (AB320799)
  • sELISA

Supplier Data

Sandwich ELISA - Human REG4 ELISA Kit (AB320799)

Example of human REG4 standard curve in Sample Diluent NS. Example of human REG4 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human REG4 ELISA Kit (AB320799)
  • sELISA

Supplier Data

Sandwich ELISA - Human REG4 ELISA Kit (AB320799)

Example of human REG4 standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Example of human REG4 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.23 pg/mL

Range

12.5 - 800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB255820

Anti-REG4 antibody [EPR22810-327]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG4 antibody [EPR22810-327] (AB255820)

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Proteins & Peptides

AB202197

Recombinant Human REG4 protein (denatured) (T7 tag N-Terminus)

SDS-PAGE - Recombinant Human REG4 protein (denatured) (T7 tag N-Terminus) (AB202197)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human REG4 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of REG4 protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human REG4 with 2.23 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.5" } ]
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Recovery

[ { "sample": "Serum", "range": "96 - 108 %", "average": "= 102" }, { "sample": "Citrate plasma", "range": "88 - 98 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "89 - 107 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "94 - 104 %", "average": "= 99" }, { "sample": "Cell culture supernatant", "range": "102 - 108 %", "average": "= 106" } ]
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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab320799-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB320799-CMP08" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB320799-CMP06" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB320799-CMP07" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB320799-CMP09" }, { "size":"20 x 1 Vial", "name":"Human REG4 Lyophilized Recombinant Protein", "number":"AB320799-CMP03" }, { "size":"10 x 600 µL", "name":"10X Human REG4 Detector Antibody", "number":"AB320799-CMP02" }, { "size":"10 x 600 µL", "name":"10X Human REG4 Capture Antibody", "number":"AB320799-CMP01" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB320799-CMP04" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB320799-CMP10" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB320799-CMP05" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab320799-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB320799-CMP08" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB320799-CMP06" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB320799-CMP07" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB320799-CMP10" }, { "size":"2 x 1 Vial", "name":"Human REG4 Lyophilized Recombinant Protein", "number":"AB320799-CMP03" }, { "size":"1 x 600 µL", "name":"10X Human REG4 Detector Antibody", "number":"AB320799-CMP02" }, { "size":"1 x 600 µL", "name":"10X Human REG4 Capture Antibody", "number":"AB320799-CMP01" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB320799-CMP04" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB320799-CMP05" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB320799-CMP11" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab320799-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB320799-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB320799-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB320799-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB320799-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human REG4 Lyophilized Recombinant Protein", "number":"AB320799-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human REG4 Detector Antibody", "number":"AB320799-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human REG4 Capture Antibody", "number":"AB320799-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB320799-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB320799-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB320799-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

REG4 also known as Reg IV or regenerating islet-derived 4 is a protein with a mass of about 19 kDa. It belongs to the regenerating gene family and has roles in cell growth and regeneration. REG4 expression occurs mainly in the gastric antrum small intestine colon and pancreas. It also appears in certain epithelial tissues particularly ones undergoing regeneration or transformation. REG4's structure includes a C-type lectin domain which suggests its potential involvement in cell adhesion or signaling.
Biological function summary

REG4 strengthens the protection of epithelial cells and supports regeneration during tissue damage or inflammation. It acts as part of cell growth and survival pathways and does not form large complexes on its own. The protein can promote proliferation and maintain integrity in epithelial tissues influencing both normal and cancerous cell behavior. The presence of REG4 might modulate cell response to environmental stress and impact cellular differentiation processes.

Pathways

REG4 impacts both the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are important for cell proliferation and survival indicating REG4's involvement in cellular stress response and oncogenesis. REG4 interacts with other proteins such as EGFR (Epidermal Growth Factor Receptor) which participate in these pathways. This protein co-activity may enhance cellular responses contributing to growth regulation and survival signaling in various tissues.

REG4 has a strong association with colorectal cancer and inflammatory bowel disease (IBD). Its overexpression has been noted in colorectal cancer tissues and can relate to tumor growth and metastasis. In the context of IBD REG4 expression connects with inflammation markers and mucosal healing processes. Proteins like MUC2 (Mucin 2) and CDX2 (Caudal Type Homeobox 2) involved in these diseases show relationships with REG4 emphasizing its role in disease progression and regulation within epithelial tissues.
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Product protocols

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Target data

Calcium-independent lectin displaying mannose-binding specificity and able to maintain carbohydrate recognition activity in an acidic environment. May be involved in inflammatory and metaplastic responses of the gastrointestinal epithelium.
See full target information REG4
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websiteProtocolBooklet
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Product promise

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