AB325080
Human Regucalcin ELISA Kit
- SimpleStep
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Human Regucalcin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Regucalcin with a sensitivity of 26.24pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
-Colormetric sandwich ELISA - 450 nm readout- works on any standard plate reader.
-Different formats for different needs: 10 x 96 plates for bulk orders.
View Alternative Names
SMP30, RGN, Regucalcin, RC, Gluconolactonase, Senescence marker protein 30, GNL, SMP-30
2 Images
- sELISA
Lab
Sandwich ELISA - Human Regucalcin ELISA Kit (AB325080)
Example of human Regucalcin standard curve in 1X Cell Extraction Buffer SSW. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Lab
Sandwich ELISA - Human Regucalcin ELISA Kit (AB325080)
Interpolated concentration of native Regucalcin was measured in duplicate at different sample concentrations. Undiluted samples are 250 µg/mL HepG2 cell extract and 62.5 µg/mL human liver tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.
Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
Product details
Human Regucalcin ELISA Kit (ab325080) is a rapid single-wash sandwich 90-min ELISA to measure Human Regucalcin in Cell Extract, Tissue Extract. This SimpleStep sensitivity is 26.24 pg/ml.
How the assay works
Human Regucalcin ELISA Kit (ab325080) SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Regucalcin (ab325080) protocol summary
1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
How the assay works
Human Regucalcin ELISA Kit (ab325080) SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Regucalcin (ab325080) protocol summary
1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Precision
[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.4" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 8.5" } ]
Recovery
[ { "sample": "Cell Lysate", "range": "94 - 101 %", "average": "= 97" }, { "sample": "Tissue Extracts", "range": "90 - 100 %", "average": "= 94" } ]
What's included?
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Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Gluconolactonase with low activity towards other sugar lactones, including gulonolactone and galactonolactone. Can also hydrolyze diisopropyl phosphorofluoridate and phenylacetate (in vitro). Calcium-binding protein. Modulates Ca(2+) signaling, and Ca(2+)-dependent cellular processes and enzyme activities (By similarity).
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