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AB213820

Human Relaxin 1 ELISA Kit

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Human Relaxin 1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Relaxin 1 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

Prorelaxin H1, RLN1

1 Images
Sandwich ELISA - Human Relaxin 1 ELISA Kit (AB213820)
  • sELISA

Supplier Data

Sandwich ELISA - Human Relaxin 1 ELISA Kit (AB213820)

Human Relaxin 1 ELISA Kit (ab213820) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

78 - 5000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human Relaxin 1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213820) is designed for the quantitative measurement of Human Relaxin 1 in cell culture supernatants, serum and plasma (heparin).

The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for Relaxin 1 has been pre-coated onto 96-well plates. Standards (E.coli, R162-Cys185(A) and K26-S53(B)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Relaxin 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Relaxin 1 amount of sample captured in plate.

Relaxin 1 is a member of relaxin-like peptide family. Relaxin gene maps to human chromosome 19 near D19Mit23. Relaxin is a peptide hormone produced by the corpora lutea of ovaries during pregnancy in many mammalian species, including man. Relaxin widens the pubic bone and facilitates labor, it also softens the cervix (cervical ripening), and relaxes the uterine musculature. However, its significance may reach much further. Relaxin affects collagen metabolism, inhibiting collagen synthesis and enhancing its breakdown by increasing matrix metalloproteinases. It also enhances angiogenesis and is a potent renal vasodilator.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "796 pg/mL", "standardDeviation": "42.98", "coefficientOfVariability": "= 5.4" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "133 pg/mL", "standardDeviation": "10.5", "coefficientOfVariability": "= 7.9" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "2390 pg/mL", "standardDeviation": "100.38", "coefficientOfVariability": "= 4.2" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "2573 pg/mL", "standardDeviation": "118.35", "coefficientOfVariability": "= 4.6" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "144 pg/mL", "standardDeviation": "10.94", "coefficientOfVariability": "= 7.6" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "819 pg/mL", "standardDeviation": "37.67", "coefficientOfVariability": "= 4.6" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213820-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB213820-CMP03", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB213820-CMP10", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB213820-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB213820-CMP05", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Plate Seal", "number":"AB213820-CMP06", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-Human Relaxin 1 antibody", "number":"AB213820-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB213820-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Relaxin 1 coated Microplate (12 x 8 wells)", "number":"AB213820-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized recombinant Human Relaxin 1 standard", "number":"AB213820-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB213820-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB213820-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Relaxin 1 also known as RLN1 is a peptide hormone that plays a role in reproductive biology. It has a mass of approximately 6 kilodaltons. Relaxin 1 is expressed in various tissues mainly the reproductive system including the ovaries placenta and uterus. It modulates connective tissue remodeling which is important during childbirth by helping to relax the pelvic ligaments. Additionally it has effects on the cardiovascular and nervous systems.
Biological function summary

The hormone influences reproductive processes through its action on tissues affecting aspects like collagen remodeling and vasodilation. It does not form part of a complex but interacts with receptors such as RXFP1 which are G-protein-coupled receptors. The binding of Relaxin 1 to RXFP1 receptors activates several downstream signaling pathways that facilitate its biological functions.

Pathways

The role of Relaxin 1 extends beyond reproductive functions. It is involved in the extracellular matrix pathway and can influence the nitric oxide signaling pathway which impacts vascular relaxation and blood flow. Relaxin 1 related proteins include RXFP1 and its family member Relaxin 2 which can bind to the same receptor and trigger similar effects.

Relaxin 1 has a connection to fibrotic diseases and cardiovascular conditions. It exhibits antifibrotic properties reducing tissue scarring in organs like the skin and lungs. In cardiovascular diseases Relaxin 1 can help improve heart functions through its vasorelaxation effects. In these disease contexts Relaxin 1 interacts with proteins involved in the extracellular matrix and nitric oxide pathways to mediate its impact.
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Product protocols

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Target data

Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix.
See full target information Prorelaxin H1
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websiteProtocolBooklet
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