AB243688

Human Relaxin-2 ELISA Kit

Recombinant
SimpleStep
What is this?

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(1 Publication)

Human Relaxin-2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Relaxin-2 with a sensitivity of 1.05 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Prorelaxin H2, RLN2

3 Images

Sandwich ELISA - Human Relaxin-2 ELISA Kit (AB243688)

sELISA

Supplier Data

Sandwich ELISA - Human Relaxin-2 ELISA Kit (AB243688)

Human Relaxin-2 Standard Curve Comparison.

Standard Curve comparison between human Relaxin-2 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human Relaxin-2 ELISA Kit (AB243688)

Interpolated concentrations of native Relaxin-2 in pooled pregnant human serum samples.

The concentrations of Relaxin-2 were measured in duplicates, interpolated from the Relaxin-2 standard curves and corrected for sample dilution. Undiluted samples are as follows : pregnant serum 6.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Relaxin-2 concentration was determined to be 1,123 pg/mL in pregnant serum.

sELISA

Supplier Data

Sandwich ELISA - Human Relaxin-2 ELISA Kit (AB243688)

Example of human Relaxin-2 standard curve in Sample Diluent NS.

The Relaxin-2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.05 pg/mL

Range

2.03 - 130 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Relaxin-2 ELISA Kit (ab243688) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Relaxin-2 protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Relaxin-2 with 1.05 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Protein", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" }, { "reproducibilityType": "Intra", "sample": "Protein", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" } ]

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Recovery

[ { "sample": "Serum", "range": "89 - 102 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "97 - 107 %", "average": "= 102" }, { "sample": "Heparin Plasma", "range": "81 - 91 %", "average": "= 87" }, { "sample": "Citrate plasma", "range": "95 - 110 %", "average": "= 104" }, { "sample": "Cell culture media", "range": "79 - 85 %", "average": "= 83" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Relaxin 2 (RLN2) also known as human relaxin is a peptide hormone with a molecular mass of approximately 6 kDa. It belongs to the insulin superfamily. This hormone is secreted primarily by the corpus luteum during pregnancy but also gets expression in other tissues like the heart and kidney. Relaxin 2 plays an important role in reproductive physiology facilitating a range of mechanical processes essential for pregnancy such as remodeling the extracellular matrix and influencing the dilation of the cervix.

Biological function summary

The role of human relaxin involves modulating tissue growth and remodeling. It works by interacting with relaxin family peptide receptors mainly RXFP1. It is not part of a larger complex but has autonomous structural and functional capabilities. The hormone influences collagen metabolism and fibroblast activity enhancing the extensibility and elasticity of the tissues where it is active.

Pathways

Researchers have identified relaxin 2 as a critical component in the relaxin signaling pathway and involved in the transforming growth factor-beta (TGF-beta) pathway. Its interaction with RXFP1 activates several intracellular signaling cascades including adenylate cyclase/cAMP pathway and phosphatidylinositol 3-kinase pathway affecting proteins like collagen and matrix metalloproteinases. These pathways play a fundamental role in connective tissue remodeling and have implications in normal and pathological states.

Studies connect relaxin 2 to conditions such as cardiac fibrosis and scleroderma. In cardiac fibrosis relaxin 2 influences matrix metalloproteinases and collagen metabolism aiming to reduce pathological tissue stiffness. In scleroderma relaxin 2's potential to modulate excessive collagen deposition links it to mitigating disease symptoms alongside proteins like TGF-beta which play a part in the disease progression.

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Product protocols

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Target data

Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix.

See full target information RLN2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of leukocyte biology 113:383-399 PubMed36801950

2023

Tumor-activated neutrophils promote metastasis in breast cancer via the G-CSF-RLN2-MMP-9 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Youjing Sheng,Weidong Peng,Yan Huang,Lanqing Cheng,Ye Meng,Louis Boafo Kwantwi,Jiezhen Yang,Jiegou Xu,Han Xiao,Julia Kzhyshkowska,Qiang Wu

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Relaxin-2 ELISA Kit

Sandwich ELISA - Human Relaxin-2 ELISA Kit (AB243688)

Sandwich ELISA - Human Relaxin-2 ELISA Kit (AB243688)

Sandwich ELISA - Human Relaxin-2 ELISA Kit (AB243688)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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