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AB246545

Human Renin ELISA Kit

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Human Renin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Renin with a sensitivity of 5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Renin, Angiotensinogenase, REN

5 Images
Sandwich ELISA - Human Renin ELISA Kit (AB246545)
  • sELISA

Supplier Data

Sandwich ELISA - Human Renin ELISA Kit (AB246545)

Example of human Renin standard curve in Sample Diluent 50BS.

The Renin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Renin ELISA Kit (AB246545)
  • sELISA

Supplier Data

Sandwich ELISA - Human Renin ELISA Kit (AB246545)

Serum from eight individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Renin concentration in neat serum was determined to be 796 pg/mL with a range of 126 – 1,757 pg/mL.

Sandwich ELISA - Human Renin ELISA Kit (AB246545)
  • sELISA

Supplier Data

Sandwich ELISA - Human Renin ELISA Kit (AB246545)

Interpolated concentrations of native Renin in human urine samples.

The concentrations of Renin were measured in duplicates, interpolated from the Renin standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 37.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Renin concentration was determined to be 2,514 pg/mL in urine.

Sandwich ELISA - Human Renin ELISA Kit (AB246545)
  • sELISA

Supplier Data

Sandwich ELISA - Human Renin ELISA Kit (AB246545)

Human Renin Standard Curve Comparison.

Standard Curve comparison between human Renin SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human Renin ELISA Kit (AB246545)
  • sELISA

Supplier Data

Sandwich ELISA - Human Renin ELISA Kit (AB246545)

Interpolated concentrations of native Renin in human serum and plasma samples.

The concentrations of Renin were measured in duplicates, interpolated from the Renin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 75%, plasma (EDTA) 75%, plasma (citrate) 75%, and plasma (heparin) 75%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Renin concentration was determined to be 528 pg/mL in serum, 482 pg/mL in plasma (EDTA), 430 pg/mL in plasma (citrate)and 460 pg/mL in plasma (heparin).

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 5 pg/mL

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Renin ELISA Kit (ab246545) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Renin protein in cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Renin with 5 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" } ]
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Recovery

[ { "sample": "Serum", "range": "109 - 117 %", "average": "= 112" }, { "sample": "EDTA Plasma", "range": "95 - 100 %", "average": "= 98" }, { "sample": "Urine", "range": "104 - 106 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "99 - 108 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "102 - 108 %", "average": "= 105" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab246545-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB246545-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB246545-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB246545-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB246545-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB246545-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Renin Lyophilized Recombinant Protein", "number":"AB246545-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Renin Capture Antibody", "number":"AB246545-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 50BS", "number":"AB246545-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB246545-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Renin Detector Antibody", "number":"AB246545-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB246545-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Renin also known as angiotensinogenase is a proteolytic enzyme with a molecular weight of approximately 40 kDa. It is mainly expressed in the juxtaglomerular cells of the kidneys. Renin plays an important role in the renin-angiotensin system where it initiates the conversion of angiotensinogen to angiotensin I. This process is the first step in a cascade leading to a series of effects in blood pressure regulation and fluid balance.
Biological function summary

The renin enzyme is responsible for blood pressure control and electrolyte homeostasis. It acts as a significant regulator within the renin-angiotensin aldosterone system (RAAS). By catalyzing the formation of angiotensin I from angiotensinogen renin sets off a sequence of reactions that influence vascular tone and sodium reabsorption. The activity of renin significantly interacts with various cardiovascular functions alongside complex regulatory feedback from other hormones such as aldosterone.

Pathways

Renin is a pivotal part of RAAS and interlinks with multiple cardiovascular pathways. The RAAS pathway involves angiotensinogen renin angiotensin I converting enzyme (ACE) and angiotensin II. Angiotensin II then acts on its receptor to mediate vasoconstriction and aldosterone secretion. The direct relationship between renin and ACE places this enzyme in the center of blood pressure management and has implications on kidney function and homeostasis.

High renin activity has connections with hypertension and cardiovascular diseases. Elevated renin levels contribute to increased angiotensin II production causing vasoconstriction and blood pressure elevation. Disorders like renal hypertension see direct involvement of the renin-angiotensin system. Therapeutic approaches often target RAAS with angiotensin-converting enzyme inhibitors (ACE inhibitors) and angiotensin receptor blockers aiming to mitigate the renin-related impact on these diseases.
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Product protocols

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Target data

Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
See full target information REN
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websiteProtocolBooklet
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