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AB326302

Human Renin ELISA Kit, Chemiluminescent

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Human Renin ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Renin with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 2.74 - 6000 pg/ml with a sensitivity of 1.37pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

Renin, Angiotensinogenase, REN

6 Images
Sandwich ELISA - Human Renin ELISA Kit, Chemiluminescent (AB326302)
  • sELISA

Lab

Sandwich ELISA - Human Renin ELISA Kit, Chemiluminescent (AB326302)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human Renin ELISA Kit, Chemiluminescent (AB326302)
  • sELISA

Lab

Sandwich ELISA - Human Renin ELISA Kit, Chemiluminescent (AB326302)

Standard curve comparison between the Chemiluminescent Human Renin SimpleStep ELISA and colorimetric Human Renin SimpleStep ELISA (ab246545) in Sample Diluent 50BS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Renin ELISA Kit, Chemiluminescent (AB326302)
  • sELISA

Lab

Sandwich ELISA - Human Renin ELISA Kit, Chemiluminescent (AB326302)

Dynamic range comparison between the Chemiluminescent Human Renin SimpleStep ELISA, colorimetric Human Renin SimpleStep ELISA (ab246545), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human Renin ELISA Kit, Chemiluminescent (AB326302)
  • sELISA

Supplier Data

Sandwich ELISA - Human Renin ELISA Kit, Chemiluminescent (AB326302)

Serum from eight individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Renin concentration in neat serum was determined to be 796 pg/mL with a range of 126 – 1757 pg/mL.

Sandwich ELISA - Human Renin ELISA Kit, Chemiluminescent (AB326302)
  • sELISA

Supplier Data

Sandwich ELISA - Human Renin ELISA Kit, Chemiluminescent (AB326302)

Interpolated concentrations of native Renin in human serum and plasma samples.

The concentrations of Renin were measured in duplicates interpolated from the Renin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 75% plasma (EDTA) 75% plasma (citrate) 75% and plasma (heparin) 75%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Renin concentration was determined to be 528 pg/mL in serum 482 pg/mL in plasma (EDTA) 430 pg/mL in plasma (citrate)and 460 pg/mL in plasma (heparin).

Sandwich ELISA - Human Renin ELISA Kit, Chemiluminescent (AB326302)
  • sELISA

Supplier Data

Sandwich ELISA - Human Renin ELISA Kit, Chemiluminescent (AB326302)

Interpolated concentrations of native Renin in human urine samples.

The concentrations of Renin were measured in duplicates interpolated from the Renin standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 37.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Renin concentration was determined to be 2514 pg/mL in urine.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Urine

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.37 pg/mL

Range

2.74 - 6000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human Renin ELISA Kit, Chemiluminescent (ab326302) is a rapid single-wash sandwich 55-min ELISA to measure Human Renin in Serum, Plasma – Citrate, Plasma – EDTA, Plasma – Heparin, Urine with a sensitivity of 1.37 pg/mL.

How the assay works

Human Renin ELISA Kit, Chemiluminescent (ab326302) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Renin (ab326302) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Renin antibody [EPR22174-8] - BSA and Aside free (Capture) ab259627
- Anti-Renin antibody [EPR22174-213] - BSA and Aside free (Detector) ab259628
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.3" } ]
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Recovery

[ { "sample": "Serum", "range": "109 - 117 %", "average": "= 112" }, { "sample": "EDTA Plasma", "range": "95 - 100 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "102 - 108 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "99 - 108 %", "average": "= 103" }, { "sample": "Urine", "range": "104 - 106 %", "average": "= 105" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
See full target information REN
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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