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AB100634

Human Resistin ELISA Kit

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(2 Publications)

Human Resistin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Resistin in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

FIZZ3, HXCP1, RSTN, UNQ407/PRO1199, RETN, Resistin, Adipose tissue-specific secretory factor, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, Cysteine-rich secreted protein FIZZ3, ADSF

2 Images
Sandwich ELISA - Human Resistin ELISA Kit (AB100634)
  • sELISA

Supplier Data

Sandwich ELISA - Human Resistin ELISA Kit (AB100634)

Representative Standard Curve using ab100634.

Sandwich ELISA - Human Resistin ELISA Kit (AB100634)
  • sELISA

Supplier Data

Sandwich ELISA - Human Resistin ELISA Kit (AB100634)

Representative Standard Curve using ab100634.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 2 pg/mL

Range

1.4 - 400 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Resistin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Resistin in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human Resistin coated on a 96-well plate. Standards and samples are pipetted into the wells and Resistin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Resistin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Resistin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get results in 90 minutes with Human Resistin ELISA Kit (ab183364) from our SimpleStep ELISA® range.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "89 - 108 %", "average": "= 98.42" }, { "sample": "Serum", "range": "78 - 102 %", "average": "= 93.59" }, { "sample": "Plasma", "range": "79 - 103 %", "average": "= 94.31" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100634-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100634-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100634-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100634-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human Resistin Standard (lyophilized)", "number":"AB100634-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Resistin Microplate (12 x 8 wells)", "number":"AB100634-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin Concentrate", "number":"AB100634-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100634-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human Resistin", "number":"AB100634-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100634-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Resistin also known as FIZZ3 or ADSF is a protein with a mass of approximately 12.5 kDa. It is mainly expressed in adipose tissue and secreted by adipocytes. In human beings it is also found in inflammatory zones like macrophages and the placenta. Resistin functions as a signaling molecule or cytokine which plays roles in inflammation and metabolism.
Biological function summary

Resistin affects glucose homeostasis and has been shown to modulate insulin resistance. It does not function as part of a larger protein complex but instead acts through specific receptors on cell surfaces. Resistin influences several cellular processes including energy balance and the modulation of inflammatory responses.

Pathways

Several processes contribute to the detailed function of resistin. It is involved in the insulin signaling pathway and the inflammatory pathway. Resistin interacts with proteins and receptors like TLR4 which have key roles in inflammatory response signaling and metabolic regulation. These interactions can disrupt insulin signaling and glucose uptake in many cell types.

Resistin has connections to type 2 diabetes and obesity. Its role in insulin resistance makes it a significant molecule in the pathophysiology of type 2 diabetes. Additionally resistin can influence obesity through its effect on energy balance and fat distribution. Resistin's relationship with proteins like TNF-alpha and IL-6 further emphasizes its role in linking metabolic syndrome with chronic inflammatory states.
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Product protocols

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Target data

Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells (By similarity). Potentially links obesity to diabetes (By similarity). Promotes chemotaxis in myeloid cells (PubMed : 15064728).
See full target information RETN
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 14:3279-3287 PubMed28912879

2017

A crossover study of the combination therapy of metformin and exenatide or biphasic insulin aspart 30 in overweight or obese patients newly diagnosed with type 2 diabetes mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Huibiao Quan,Huachuan Zhang,Weiping Wei,Tuanyu Fang,Daoxiong Chen,Kaining Chen

Therapeutics and clinical risk management 13:739-750 PubMed28721055

2017

Chitosan oligosaccharide improves the therapeutic efficacy of sitagliptin for the therapy of Chinese elderly patients with type 2 diabetes mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Lijie Zhao,Tingli Sun,Lina Wang
View all publications
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websiteProtocolBooklet
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