Human Resistin ELISA Kit
- SimpleStep
- What is this?
Be the first to review this product! Submit a review
|
(4 Publications)
Human Resistin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Resistin with a sensitivity of 24 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
View Alternative Names
FIZZ3, HXCP1, RSTN, UNQ407/PRO1199, RETN, Resistin, Adipose tissue-specific secretory factor, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, Cysteine-rich secreted protein FIZZ3, ADSF
- ELISA
Supplier Data
ELISA - Human Resistin ELISA Kit (AB183364)
- sELISA
Supplier Data
Sandwich ELISA - Human Resistin ELISA Kit (AB183364)
Example of Resistin standard curve in cell culture media.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human Resistin ELISA Kit (AB183364)
Example of Resistin standard curve.
Background-subtracted data values (mean +/- SD) are graphed
- sELISA
Supplier Data
Sandwich ELISA - Human Resistin ELISA Kit (AB183364)
Levels of Resistin in Normal Serum.
The levels of native Resistin in serum samples were tested from ten individual healthy donors. Levels were interpolated from the standard curve in Sample Diluent NS. The mean level of Resistin was 2.6 ng/mL with a range of 1.6 to 5.3 ng/mL and a standard deviation of 1 ng/mL.
Reactivity data
Product details
Human Resistin ELISA Kit ab183364 is a rapid single-wash 90-min Sandwich ELISA to measure Human Resistin in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 24 pg/mL.
How the assay works
Human Resistin SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Resistin ELISA Kit ab183364 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Resistin, otherwise known as Found in Inflammatory Zone 3 (FIZZ3), and Adipocyte Secreted Factor (ADSF), is a 114 amino-acid cysteine-rich protein. It can be found as a high molecular weight hexamer or as a more active, lower molecular weight complex. Resistin is the best known member of the family of Resistin-like Molecules (RELMs), which include RELM-alpha/FIZZ1 and RELM-beta/FIZZE 2, both described only in rodents. Furthermore, resistin has been categorized as part of the group of adipokines hormones, which are known to be involved in the development of obesity, insulin resistance, diabetes, inflammation, autoimmune disease and metabolic syndrome.
In Humans, Resistin is a pro-inflammatory molecule primarily secreted by macrophages and mononuclear bone marrow cells in response to inflammation, hyperglycemia, and growth hormones. IL-1, IL-6, TNF-alpha as well as lipopolysaccharide (LPS) are strong inducers of resistin as observed in animal models of hepatic inflammation and necrosis. High levels of resistin are also observed in patients with pancreatitis and with systemic lupus erythematosus.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Resistin affects glucose homeostasis and has been shown to modulate insulin resistance. It does not function as part of a larger protein complex but instead acts through specific receptors on cell surfaces. Resistin influences several cellular processes including energy balance and the modulation of inflammatory responses.
Pathways
Several processes contribute to the detailed function of resistin. It is involved in the insulin signaling pathway and the inflammatory pathway. Resistin interacts with proteins and receptors like TLR4 which have key roles in inflammatory response signaling and metabolic regulation. These interactions can disrupt insulin signaling and glucose uptake in many cell types.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (4)
Recent publications for all applications. Explore the full list and refine your search
International journal of surgery (London, England) 110:5434-5443 PubMed38833355
2024
Applications
Unspecified application
Species
Unspecified reactive species
Journal of personalized medicine 12: PubMed35629157
2022
Applications
Unspecified application
Species
Unspecified reactive species
Scientific reports 11:20794 PubMed34675320
2021
Applications
Unspecified application
Species
Unspecified reactive species
Cureus 13:e18450 PubMed34745775
2021
Applications
Unspecified application
Species
Unspecified reactive species
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com