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Human Resistin ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Resistin in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.

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Images

Sandwich ELISA - Human Resistin ELISA Kit (AB183364), expandable thumbnail
  • Sandwich ELISA - Human Resistin ELISA Kit (AB183364), expandable thumbnail
  • Sandwich ELISA - Human Resistin ELISA Kit (AB183364), expandable thumbnail

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Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

78.1 - 5000 pg/mL

Assay time

1h 30m

Sensitivity

= 24 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells (By similarity). Potentially links obesity to diabetes (By similarity). Promotes chemotaxis in myeloid cells (PubMed:15064728).

Alternative names

What's included?

1 x 96 Tests
Components
10X Human Resistin Capture Antibody
1 x 600 µL
10X Human Resistin Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
Antibody Diluent CPI2
1 x 6 mL
Human Resistin Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Human Resistin ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Resistin in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

78.1 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate

Sensitivity

= 24 pg/mL

Precision

Intra assay

Sample

Serum

n

9

mean

-

SD

-

C.V.

3

Inter assay

Sample

Serum

n

3

mean

-

SD

-

C.V.

6

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 94

Range

88 - 100 %

Sample type

EDTA Plasma

Average %

= 96

Range

88 - 104 %

Sample type

Heparin Plasma

Average %

= 95

Range

84 - 110 %

Sample type

Citrate plasma

Average %

= 78

Range

68 - 90 %

Sample type

Cell culture media

Average %

= 106

Range

106 - 107 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human Resistin ELISA kit (ab183364) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Resistin protein in human serum, plasma and cell culture supernatants. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Resistin with 24 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.

Resistin, otherwise known as Found in Inflammatory Zone 3 (FIZZ3), and Adipocyte Secreted Factor (ADSF), is a 114 amino-acid cysteine-rich protein. It can be found as a high molecular weight hexamer or as a more active, lower molecular weight complex. Resistin is the best known member of the family of Resistin-like Molecules (RELMs), which include RELM-alpha/FIZZ1 and RELM-beta/FIZZE 2, both described only in rodents. Furthermore, resistin has been categorized as part of the group of adipokines hormones, which are known to be involved in the development of obesity, insulin resistance, diabetes, inflammation, autoimmune disease and metabolic syndrome.

In Humans, Resistin is a pro-inflammatory molecule primarily secreted by macrophages and mononuclear bone marrow cells in response to inflammation, hyperglycemia, and growth hormones. IL-1, IL-6, TNF-alpha as well as lipopolysaccharide (LPS) are strong inducers of resistin as observed in animal models of hepatic inflammation and necrosis. High levels of resistin are also observed in patients with pancreatitis and with systemic lupus erythematosus.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Resistin also known as FIZZ3 or ADSF is a protein with a mass of approximately 12.5 kDa. It is mainly expressed in adipose tissue and secreted by adipocytes. In human beings it is also found in inflammatory zones like macrophages and the placenta. Resistin functions as a signaling molecule or cytokine which plays roles in inflammation and metabolism.

Biological function summary

Resistin affects glucose homeostasis and has been shown to modulate insulin resistance. It does not function as part of a larger protein complex but instead acts through specific receptors on cell surfaces. Resistin influences several cellular processes including energy balance and the modulation of inflammatory responses.

Pathways

Several processes contribute to the detailed function of resistin. It is involved in the insulin signaling pathway and the inflammatory pathway. Resistin interacts with proteins and receptors like TLR4 which have key roles in inflammatory response signaling and metabolic regulation. These interactions can disrupt insulin signaling and glucose uptake in many cell types.

Associated diseases and disorders

Resistin has connections to type 2 diabetes and obesity. Its role in insulin resistance makes it a significant molecule in the pathophysiology of type 2 diabetes. Additionally resistin can influence obesity through its effect on energy balance and fat distribution. Resistin's relationship with proteins like TNF-alpha and IL-6 further emphasizes its role in linking metabolic syndrome with chronic inflammatory states.

Product promise

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3 product images

  • Sandwich ELISA - Human Resistin ELISA Kit (ab183364), expandable thumbnail

    Sandwich ELISA - Human Resistin ELISA Kit (ab183364)

    Example of Resistin standard curve.

    Background-subtracted data values (mean +/- SD) are graphed

  • Sandwich ELISA - Human Resistin ELISA Kit (ab183364), expandable thumbnail

    Sandwich ELISA - Human Resistin ELISA Kit (ab183364)

    Levels of Resistin in Normal Serum.

    The levels of native Resistin in serum samples were tested from ten individual healthy donors. Levels were interpolated from the standard curve in Sample Diluent NS. The mean level of Resistin was 2.6 ng/mL with a range of 1.6 to 5.3 ng/mL and a standard deviation of 1 ng/mL.

  • Sandwich ELISA - Human Resistin ELISA Kit (ab183364), expandable thumbnail

    Sandwich ELISA - Human Resistin ELISA Kit (ab183364)

    Example of Resistin standard curve in cell culture media.

    Background-subtracted data values (mean +/- SD) are graphed.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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