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AB108896

Human Resistin ELISA Kit (RETN)

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(3 Publications)

Human Resistin ELISA Kit (RETN) is a Sandwich ELISA for the measurement of Human Resistin (RETN) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

FIZZ3, HXCP1, RSTN, UNQ407/PRO1199, RETN, Resistin, Adipose tissue-specific secretory factor, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, Cysteine-rich secreted protein FIZZ3, ADSF

1 Images
Sandwich ELISA - Human Resistin ELISA Kit (RETN) (AB108896)
  • sELISA

Supplier Data

Sandwich ELISA - Human Resistin ELISA Kit (RETN) (AB108896)

Representative Standard Curve using ab108896.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Urine, Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.2 ng/mL

Range

0.25 - 16 ng/mL

Assay time

5h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Resistin (RETN) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Resistin concentrations in Human plasma, serum, urine, saliva and cell culture supernatants.

A Resistin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Resistin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Resistin captured in plate.

Get results in 90 minutes with Human Resistin ELISA Kit (ab183364) from our SimpleStep ELISA® range.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.2" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108896-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108896-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Resistin Microplate (12 x 8 well strips)", "number":"AB108896-CMP03", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108896-CMP09", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human Resistin Antibody", "number":"AB108896-CMP01", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108896-CMP08", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108896-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Resistin Standard", "number":"AB108896-CMP04", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108896-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent M Concentrate", "number":"AB108896-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Resistin also known as FIZZ3 or ADSF is a protein with a mass of approximately 12.5 kDa. It is mainly expressed in adipose tissue and secreted by adipocytes. In human beings it is also found in inflammatory zones like macrophages and the placenta. Resistin functions as a signaling molecule or cytokine which plays roles in inflammation and metabolism.
Biological function summary

Resistin affects glucose homeostasis and has been shown to modulate insulin resistance. It does not function as part of a larger protein complex but instead acts through specific receptors on cell surfaces. Resistin influences several cellular processes including energy balance and the modulation of inflammatory responses.

Pathways

Several processes contribute to the detailed function of resistin. It is involved in the insulin signaling pathway and the inflammatory pathway. Resistin interacts with proteins and receptors like TLR4 which have key roles in inflammatory response signaling and metabolic regulation. These interactions can disrupt insulin signaling and glucose uptake in many cell types.

Resistin has connections to type 2 diabetes and obesity. Its role in insulin resistance makes it a significant molecule in the pathophysiology of type 2 diabetes. Additionally resistin can influence obesity through its effect on energy balance and fat distribution. Resistin's relationship with proteins like TNF-alpha and IL-6 further emphasizes its role in linking metabolic syndrome with chronic inflammatory states.
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Product protocols

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Target data

Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells (By similarity). Potentially links obesity to diabetes (By similarity). Promotes chemotaxis in myeloid cells (PubMed : 15064728).
See full target information RETN
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

JOR spine 8:e70073 PubMed40386495

2025

An Extracellular Matrix Hydrogel Restores Disc Volume and Alleviates Axial Hypersensitivity in a Rat Model of Disc-Associated Pain.

Applications

Unspecified application

Species

Unspecified reactive species

David J Lillyman,Evie C Reddick,Kayla E Ney,Sydney M Caparaso,Rebecca A Wachs

JOR spine 5:e1212 PubMed36203864

2022

Axial hypersensitivity is associated with aberrant nerve sprouting in a novel model of disc degeneration in female Sprague Dawley rats.

Applications

Unspecified application

Species

Unspecified reactive species

David J Lillyman,Fei San Lee,Evie C Barnett,Tyler J Miller,Moreno Lozano Alvaro,Henry C Drvol,Rebecca A Wachs

Journal of diabetes research 2017:2643079 PubMed29138754

2017

The Relation between Periodontopathogenic Bacterial Levels and Resistin in the Saliva of Obese Type 2 Diabetic Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Natheer Al-Rawi,Farah Al-Marzooq
View all publications
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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