JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB108764

Human Anti-Respiratory syncytial virus IgA ELISA Kit (RSV)

Be the first to review this product! Submit a review

|

(0 Publication)

Human Anti-Respiratory syncytial virus IgA ELISA Kit (RSV) is an indirect ELISA for the qualitative detection of IgA class antibodies against Respiratory syncytial virus (RSV) in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
- Cut-off, positive and negative controls included

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Indirect

Assay Platform

Microplate

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's anti-Respiratory syncytial virus (RSV) IgA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgA class antibodies against Respiratory syncytial virus in Human serum and plasma.

A 96-well plate has been precoated with Respiratory syncytial virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgA conjugate is added to the wells, which binds to the immobilized Respiratory syncytial virus -specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Respiratory syncytial virus IgA sample captured in plate.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108764-1x96Tests", "assetComponentDetails": [ { "size":"1 x 2 mL", "name":"Respiratory syncytial virus IgA Positive Control", "number":"AB108764-CMP03", "productcode":"" }, { "size":"1 x 3 mL", "name":"Respiratory syncytial virus IgA Cut-off Control", "number":"AB108764-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Foil cover", "number":"AB108764-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Respiratory syncytial virus (IgA) Coated Microplate (12 x 8 wells)", "number":"AB108764-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"Respiratory syncytial virus anti-IgA HRP Conjugate", "number":"AB108764-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB108764-CMP06", "productcode":"" }, { "size":"1 x 100 mL", "name":"IgA Sample Diluent", "number":"AB108764-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108764-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Washing Solution", "number":"AB108764-CMP10", "productcode":"" }, { "size":"1 x 2 mL", "name":"Respiratory syncytial virus IgA Negative Control", "number":"AB108764-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108764-CMP08", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Respiratory Syncytial Virus (RSV) IgA is a type of immunoglobulin A specific to the RSV. This antibody also known as secretory IgA plays a role in mucosal immunity. IgA has two isotopes: IgA1 and IgA2 with IgA2 mostly found in mucosal areas. The molecular weight of IgA is approximately 160 kDa when in its secretory form. Expression occurs predominantly in mucosal surfaces such as respiratory tracts intestines and tears where it acts as the first line of defense against pathogens like RSV.
Biological function summary

RSV IgA acts as an important protector in mucosal areas preventing viral infections at entry points. It is part of the immune system’s humoral response contributing to neutralization and clearance of pathogens. This protein does not work in isolation but forms complexes with the secretory component which shields it from proteolytic degradation and aids in its transport across epithelial layers. Through its protective function RSV IgA reduces the likelihood of systemic spread of viruses by confining them to mucosal surfaces.

Pathways

The involvement of RSV IgA in the mucosal immune response is central to maintaining the host's defense against respiratory pathogens. It participates in the immune exclusion pathway which minimizes viral entry and colonization. RSV IgA interacts with other immunoglobulins such as IgG and IgM working collectively to mount effective immune responses. Additionally it engages in signal transduction associated with the polymeric immunoglobulin receptor (pIgR) facilitating its transcytosis across epithelial cells.

RSV IgA is closely linked to respiratory diseases such as bronchiolitis caused by RSV infection. Its presence in sufficient quantity is critical in preventing severe RSV-related lower respiratory tract infections. Insufficient IgA levels can lead to increased susceptibility and severity of these infections. It is also connected to chronic obstructive pulmonary disease (COPD) where impaired mucosal IgA response may contribute to the disease progression. In these contexts RSV IgA works alongside proteins such as pIgR and other components of the immune system to modulate immune responses and control disease outcomes.
style
left-column

Product protocols

style
left-column

Target data

style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB108743

Human Anti-Influenza virus A IgA ELISA Kit

  • 0
  • 0
Assay Kits

AB108745

Human Anti-Influenza virus A IgG ELISA Kit

  • 0
  • 0
Assay Kits

AB108765

Human Anti-Respiratory syncytial virus IgG ELISA Kit (RSV)

  • 0
  • 0
Assay Kits

AB108746

Human Anti-Influenza virus B IgG ELISA Kit

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com