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AB108765

Human Anti-Respiratory syncytial virus IgG ELISA Kit (RSV)

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(5 Publications)

Human Anti-Respiratory syncytial virus IgG ELISA Kit (RSV) is an indirect ELISA for the qualitative detection of IgG class antibodies against Respiratory syncytial virus (RSV) in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
- Cut-off, positive and negative controls included

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Indirect

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's anti-Respiratory syncytial virus (RSV) IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Respiratory syncytial virus in Human serum and plasma.

A 96-well plate has been precoated with Respiratory syncytial virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Respiratory syncytial virus -specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Respiratory syncytial virus IgG sample captured in plate.

The antigen preparation (inactivated) was virus and viral components from Vero cell culture purified by centrifugation.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108765-1x96Tests", "assetComponentDetails": [ { "size":"1 x 2 mL", "name":"Respiratory syncytial virus IgG Negative Control", "number":"AB108765-CMP03", "productcode":"" }, { "size":"1 x 3 mL", "name":"Respiratory syncytial virus IgG Cut-off Control", "number":"AB108765-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Respiratory syncytial virus Coated Microplate (12 x 8 wells)", "number":"AB108765-CMP04", "productcode":"" }, { "size":"1 x 2 mL", "name":"Respiratory syncytial virus IgG Positive Control", "number":"AB108765-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"Respiratory syncytial virus anti-IgG HRP Conjugate", "number":"AB108765-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover foil", "number":"AB108765-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB108765-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108765-CMP08", "productcode":"" }, { "size":"1 x 100 mL", "name":"IgG Sample Diluent", "number":"AB108765-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108765-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Washing Solution", "number":"AB108765-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Respiratory Syncytial Virus (RSV) IgG refers to the specific immunoglobulin G antibodies targeting the RSV virus especially important in measuring immune response to RSV infection or vaccination. These antibodies typically recognize antigens presented by the RSV virus helping in neutralization. The mass of IgG molecules is approximately 150 kDa and they are expressed in the serum and extracellular fluids across various body tissues. The measurement of IgG levels using an anti-RSV IgG ELISA test provides a quantitative analysis of immunity gained either from infection or from vaccines.
Biological function summary

The detection and quantification of RSV-specific IgG contribute primarily to the adaptive immune response against the virus. IgG antibodies play a role in opsonization and neutralization of viral particles preventing the infection of host cells. While not forming a larger complex with other proteins by structural definition IgG functionality involves interactions with other components of the immune system including Fc receptors on immune effector cells.

Pathways

IgG antibodies against RSV occupy a critical role in humoral immune pathways closely related to Fc receptor-mediated processes which facilitate phagocytosis and antibody-dependent cellular cytotoxicity (ADCC). These pathways often involve interactions with proteins such as complement factors and Fcγ receptors which aid in the clearance of viral particles and infected cells.

RSV IgG levels help in diagnosing and monitoring respiratory infections caused by RSV particularly in vulnerable groups like infants and the elderly. Since RSV can lead to severe lower respiratory tract infections understanding IgG dynamics is important for assessing protection and susceptibility. The presence and quantity of these antibodies are also significant in disorders such as bronchiolitis and pneumonia where collaboration with proteins like cytokines and other immunoglobulins occurs during immune responses to infection.
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Product protocols

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Target data

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Microbiology spectrum :e0211524 PubMed39470275

2024

Clinical validation of an RSV neutralization assay and analysis of cross-sectional sera associated with 2021-2023 RSV outbreaks to investigate the immunity debt hypothesis.

Applications

Unspecified application

Species

Unspecified reactive species

Eli A Piliper,Jonathan C Reed,Alexander L Greninger

JCI insight 8: PubMed37159271

2023

Human T cells efficiently control RSV infection.

Applications

Unspecified application

Species

Unspecified reactive species

Chandrav De,Raymond J Pickles,Wenbo Yao,Baolin Liao,Allison Boone,Mingyu Choi,Diana M Battaglia,Frederic B Askin,Jason K Whitmire,Guido Silvestri,J Victor Garcia,Angela Wahl

Nature communications 10:2331 PubMed31133680

2019

The viral protein corona directs viral pathogenesis and amyloid aggregation.

Applications

Unspecified application

Species

Unspecified reactive species

Kariem Ezzat,Maria Pernemalm,Sandra Pålsson,Thomas C Roberts,Peter Järver,Aleksandra Dondalska,Burcu Bestas,Michal J Sobkowiak,Bettina Levänen,Magnus Sköld,Elizabeth A Thompson,Osama Saher,Otto K Kari,Tatu Lajunen,Eva Sverremark Ekström,Caroline Nilsson,Yevheniia Ishchenko,Tarja Malm,Matthew J A Wood,Ultan F Power,Sergej Masich,Anders Lindén,Johan K Sandberg,Janne Lehtiö,Anna-Lena Spetz,Samir El Andaloussi

Nature medicine 25:591-596 PubMed30886409

2019

The repertoire of maternal anti-viral antibodies in human newborns.

Applications

Unspecified application

Species

Unspecified reactive species

Christian Pou,Dieudonné Nkulikiyimfura,Ewa Henckel,Axel Olin,Tadepally Lakshmikanth,Jaromir Mikes,Jun Wang,Yang Chen,Anna Karin Bernhardsson,Anna Gustafsson,Kajsa Bohlin,Petter Brodin

The Journal of general virology 96:782-792 PubMed25537374

2014

Evidence for an intranasal immune response to human respiratory syncytial virus infection in cynomolgus macaques.

Applications

Unspecified application

Species

Unspecified reactive species

Clément Grandin,Marianne Lucas-Hourani,Marine Clavel,Fabrice Taborik,Astrid Vabret,Frédéric Tangy,Hugues Contamin,Pierre-Olivier Vidalain
View all publications
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