Human Anti-Respiratory syncytial virus IgG ELISA Kit (RSV) is an indirect ELISA for the qualitative detection of IgG class antibodies against Respiratory syncytial virus (RSV) in human plasma and serum samples.
- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
- Cut-off, positive and negative controls included
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
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Human Anti-Respiratory syncytial virus IgG ELISA Kit (RSV) is an indirect ELISA for the qualitative detection of IgG class antibodies against Respiratory syncytial virus (RSV) in human plasma and serum samples.
- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
- Cut-off, positive and negative controls included
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 24 | C.V. 8.5 |
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 12 | C.V. 6 |
Abcam's anti-Respiratory syncytial virus (RSV) IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Respiratory syncytial virus in Human serum and plasma.
A 96-well plate has been precoated with Respiratory syncytial virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Respiratory syncytial virus -specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Respiratory syncytial virus IgG sample captured in plate.
The antigen preparation (inactivated) was virus and viral components from Vero cell culture purified by centrifugation.
Respiratory Syncytial Virus (RSV) IgG refers to the specific immunoglobulin G antibodies targeting the RSV virus especially important in measuring immune response to RSV infection or vaccination. These antibodies typically recognize antigens presented by the RSV virus helping in neutralization. The mass of IgG molecules is approximately 150 kDa and they are expressed in the serum and extracellular fluids across various body tissues. The measurement of IgG levels using an anti-RSV IgG ELISA test provides a quantitative analysis of immunity gained either from infection or from vaccines.
The detection and quantification of RSV-specific IgG contribute primarily to the adaptive immune response against the virus. IgG antibodies play a role in opsonization and neutralization of viral particles preventing the infection of host cells. While not forming a larger complex with other proteins by structural definition IgG functionality involves interactions with other components of the immune system including Fc receptors on immune effector cells.
IgG antibodies against RSV occupy a critical role in humoral immune pathways closely related to Fc receptor-mediated processes which facilitate phagocytosis and antibody-dependent cellular cytotoxicity (ADCC). These pathways often involve interactions with proteins such as complement factors and Fcγ receptors which aid in the clearance of viral particles and infected cells.
RSV IgG levels help in diagnosing and monitoring respiratory infections caused by RSV particularly in vulnerable groups like infants and the elderly. Since RSV can lead to severe lower respiratory tract infections understanding IgG dynamics is important for assessing protection and susceptibility. The presence and quantity of these antibodies are also significant in disorders such as bronchiolitis and pneumonia where collaboration with proteins like cytokines and other immunoglobulins occurs during immune responses to infection.
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