Human Retinol Binding Protein 4 ELISA Kit
- Recombinant
- SimpleStep
- What is this?
Be the first to review this product! Submit a review
|
(2 Publications)
Human Retinol Binding Protein 4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Retinol Binding Protein 4 with a sensitivity of 2.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
PRO2222, RBP4, Retinol-binding protein 4, Plasma retinol-binding protein, PRBP, RBP
- ELISA
Supplier Data
ELISA - Human Retinol Binding Protein 4 ELISA Kit (AB196264)
- sELISA
Supplier Data
Sandwich ELISA - Human Retinol Binding Protein 4 ELISA Kit (AB196264)
Interpolated concentrations of native RBP4 in human serum and plasma samples.
The concentrations of RBP4 were measured in duplicates, interpolated from the RBP4 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 400,000, plasma (citrate) 1 : 200,000, plasma (EDTA) 1 : 100,000, and plasma (heparin) 1 : 200,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RBP4 concentration was determined to be 22.2 Âμg/mL in serum, 21.9 Âμg/mL in plasma (citrate), 15.1 Âμg/mL in plasma (EDTA) and 52.9 Âμg/mL in plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Human Retinol Binding Protein 4 ELISA Kit (AB196264)
Example of human RBP4 standard curve in Sample Diluent NS.
The RBP4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human Retinol Binding Protein 4 ELISA Kit (AB196264)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RBP4 concentration was determined to be 44 Âμg/mL with a range of 28 â€" 67 Âμg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human Retinol Binding Protein 4 ELISA Kit (AB196264)
Bovine serum was prepared at 1% and diluted 2- fold for 8 points.
Bovine reactivity was seen at serum levels greater than 0.03%.
- sELISA
Supplier Data
Sandwich ELISA - Human Retinol Binding Protein 4 ELISA Kit (AB196264)
Human RBP-4 Competitor standard curve comparison.
Standard curve comparison between Human RBP4 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
- sELISA
Supplier Data
Sandwich ELISA - Human Retinol Binding Protein 4 ELISA Kit (AB196264)
Interpolated concentrations of native RBP4 in human saliva, urine, milk, and cell culture supernatant samples.
The concentrations of RBP4 were measured in duplicates, interpolated from the RBP4 standard curves and corrected for sample dilution. Undiluted samples are as follows : Â saliva 1 : 80, urine 1 : 160, milk 1 : 1000, and HEPG2 cell culture supernatant 1 : 1000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RBP4 concentration was determined to be 16.2 ng/mL in saliva, 66.3 ng/mL in urine, 111 ng/mL in milk and 1071 ng/mL in HEPG2 cell culture supernatant.
Reactivity data
Product details
Human Retinol Binding Protein 4 ELISA Kit ab196264 is a rapid single-wash 90-min Sandwich ELISA to measure Human Retinol Binding Protein 4 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, milk, saliva, serum, urine. This SimpleStep sensitivity is 2.6 pg/mL.
How the assay works
Human Retinol Binding Protein 4 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Retinol Binding Protein 4 ELISA Kit ab196264 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-RBP4 antibody [EPR5879] - BSA and Azide free (Capture) ab244894
- Anti-RBP4 antibody [EPR20724-227] - BSA and Azide free (Detector) ab244894
Retinol-binding protein 4 (RBP4) is a circulating protein that binds to and transports retinol (vitamin A) in serum. The RBP4-retinol complex further interacts with prealbumin (also known as transthyretin) which increases its half-life in circulation. RBP4 is produced by hepatocytes and adipocytes. Loss of functional RBP4 protein results in serum retinol deficiency which can impact vision.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RBP4 plays a role as a transporter of vitamin A from the liver to tissues where it is needed. It forms complexes with transthyretin in the blood which prevents kidney filtration and subsequent loss of retinol. This function ensures the proper delivery and availability of retinol essential for vision cellular differentiation and immune system function. The stability of the retinol-RBP4 complex is important to maintaining sufficient vitamin A levels in the body.
Pathways
RBP4 is significant in the vitamin A metabolism pathway and affects the retinoid signaling pathways. These pathways are critical for processes such as vision and skin health. RBP4 interacts with proteins like lecithin retinol acyltransferase (LRAT) in these pathways which are responsible for esterifying retinol into retinyl esters a storage form of vitamin A. These interactions ensure the efficient transport and storage of vitamin A and that it is accessible when needed by different tissues.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (2)
Recent publications for all applications. Explore the full list and refine your search
International journal of molecular sciences 23: PubMed35216460
2022
Applications
Unspecified application
Species
Unspecified reactive species
Maternal & child nutrition 16:e12886 PubMed31702104
2019
Applications
Unspecified application
Species
Unspecified reactive species
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com