Human Retinol Binding Protein 4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Retinol Binding Protein 4 with a sensitivity of 2.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- International journal of molecular sciences 23:2022Urinary Proteomic Signature in Acute Decompensated Heart Failure: Advances into Molecular Pathophysiology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElisa Diaz-Riera,Maísa García-Arguinzonis,Laura López,Xavier Garcia-Moll,Lina Badimon,Teresa PadroPubMed 35216460
- Maternal & child nutrition 16:e128862019Nutritional status of Tajik children and women: Transition towards a double burden of malnutrition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTanja Barth-Jaeggi,Lizelle Zandberg,Mutribjon Bahruddinov,Sabine Kiefer,Sherali Rahmarulloev,Kaspar WyssPubMed 31702104
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 15.6 - 1000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 2.6 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Retinol-binding protein that mediates retinol transport in blood plasma (PubMed:5541771). Delivers retinol from the liver stores to the peripheral tissues (Probable). Transfers the bound all-trans retinol to STRA6, that then facilitates retinol transport across the cell membrane (PubMed:22665496).
Alternative names
PRO2222, RBP4, Retinol-binding protein 4, Plasma retinol-binding protein, PRBP, RBP
What's included?
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Human Retinol Binding Protein 4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Retinol Binding Protein 4 with a sensitivity of 2.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 15.6 - 1000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 2.6 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 5 | mean - | SD - | C.V. 5.1 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 8.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Milk | Average % = 103 | Range 100 - 108 % |
Sample type Serum | Average % = 109 | Range 104 - 111 % |
Sample type EDTA Plasma | Average % = 108 | Range 106 - 110 % |
Sample type Urine | Average % = 110 | Range 106 - 113 % |
Sample type Saliva | Average % = 104 | Range 99 - 108 % |
Sample type Heparin Plasma | Average % = 103 | Range 91 - 110 % |
Sample type Citrate plasma | Average % = 109 | Range 106 - 114 % |
Sample type Cell culture media | Average % = 101 | Range 99 - 102 % |
Sample type serum free media | Average % = 103 | Range 93 - 113 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Retinol Binding Protein 4 ELISA Kit (ab196264) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Retinol Binding Protein 4 protein in edta plasma, milk, saliva, serum, urine, cit plasma, hep plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Retinol Binding Protein 4 with 2.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Retinol-binding protein 4 (RBP4) is a circulating protein that binds to and transports retinol (vitamin A) in serum. The RBP4-retinol complex further interacts with prealbumin (also known as transthyretin) which increases its half-life in circulation. RBP4 is produced by hepatocytes and adipocytes. Loss of functional RBP4 protein results in serum retinol deficiency which can impact vision.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RBP4 or retinol-binding protein 4 is a carrier protein that is essential for the transport of retinol or vitamin A in the bloodstream. Also known by the names RBP or plasma retinol-binding protein this protein has a molecular weight of approximately 21 kDa. RBP4 is mainly expressed in the liver and secreted into the blood where it binds to retinol and protects it from oxidation. The structure of RBP4 allows it to fit into binding pockets facilitating the transport of retinol to peripheral tissues.
- Biological function summary
RBP4 plays a role as a transporter of vitamin A from the liver to tissues where it is needed. It forms complexes with transthyretin in the blood which prevents kidney filtration and subsequent loss of retinol. This function ensures the proper delivery and availability of retinol essential for vision cellular differentiation and immune system function. The stability of the retinol-RBP4 complex is important to maintaining sufficient vitamin A levels in the body.
- Pathways
RBP4 is significant in the vitamin A metabolism pathway and affects the retinoid signaling pathways. These pathways are critical for processes such as vision and skin health. RBP4 interacts with proteins like lecithin retinol acyltransferase (LRAT) in these pathways which are responsible for esterifying retinol into retinyl esters a storage form of vitamin A. These interactions ensure the efficient transport and storage of vitamin A and that it is accessible when needed by different tissues.
- Associated diseases and disorders
RBP4 is linked to the development of insulin resistance and type 2 diabetes. Elevated RBP4 levels correlate with these conditions and highlight its role in metabolic processes. Studies also show a connection between altered RBP4 levels and obesity with insulin resistance often occurring in conjunction with obesity. The protein is implicated in these metabolic disorders through its interactions with insulin-regulating proteins which influence glucose homeostasis.
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6 product images
Sandwich ELISA - Human Retinol Binding Protein 4 ELISA Kit (ab196264)
Human RBP-4 Competitor standard curve comparison.
Standard curve comparison between Human RBP4 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human Retinol Binding Protein 4 ELISA Kit (ab196264)
Example of human RBP4 standard curve in Sample Diluent NS.
The RBP4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Retinol Binding Protein 4 ELISA Kit (ab196264)
Interpolated concentrations of native RBP4 in human serum and plasma samples.
The concentrations of RBP4 were measured in duplicates, interpolated from the RBP4 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:400,000, plasma (citrate) 1:200,000, plasma (EDTA) 1: 100,000, and plasma (heparin) 1:200,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RBP4 concentration was determined to be 22.2 Âμg/mL in serum, 21.9 Âμg/mL in plasma (citrate), 15.1 Âμg/mL in plasma (EDTA) and 52.9 Âμg/mL in plasma (heparin).
Sandwich ELISA - Human Retinol Binding Protein 4 ELISA Kit (ab196264)
Interpolated concentrations of native RBP4 in human saliva, urine, milk, and cell culture supernatant samples.
The concentrations of RBP4 were measured in duplicates, interpolated from the RBP4 standard curves and corrected for sample dilution. Undiluted samples are as follows: Â saliva 1:80, urine 1: 160, milk 1:1000, and HEPG2 cell culture supernatant 1:1000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RBP4 concentration was determined to be 16.2 ng/mL in saliva, 66.3 ng/mL in urine, 111 ng/mL in milk and 1071 ng/mL in HEPG2 cell culture supernatant.
Sandwich ELISA - Human Retinol Binding Protein 4 ELISA Kit (ab196264)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RBP4 concentration was determined to be 44 Âμg/mL with a range of 28 â€" 67 Âμg/mL.
Sandwich ELISA - Human Retinol Binding Protein 4 ELISA Kit (ab196264)
Bovine serum was prepared at 1% and diluted 2- fold for 8 points.
Bovine reactivity was seen at serum levels greater than 0.03%.
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