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AB108897

Human Retinol binding protein 4 ELISA Kit (RBP4)

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(5 Publications)

Human Retinol binding protein 4 ELISA Kit (RBP4) is a Sandwich (quantitative) ELISA for the measurement of Human Retinol binding protein 4 (RBP4) in Human in Biofluids, Cell/Tissue Extracts samples.

View Alternative Names

PRO2222, RBP4, Retinol-binding protein 4, Plasma retinol-binding protein, PRBP, RBP

1 Images
Sandwich ELISA - Human Retinol binding protein 4 ELISA Kit (RBP4) (AB108897)
  • sELISA

Supplier Data

Sandwich ELISA - Human Retinol binding protein 4 ELISA Kit (RBP4) (AB108897)

Representative Standard Curve Using ab108897.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine, Cell Lysate, Tissue

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 8.3 ng/mL

Range

12.5 - 800 ng/mL

Assay time

4h

Assay Platform

Microplate

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Reactivity data

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Product details

Abcam's Retinol binding protein 4 (RBP4) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Retinol binding protein 4 concentrations in plasma, serum, saliva, urine, milk, cerebrospinal fluid and cell culture supernatants.

A Retinol binding protein 4 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Retinol binding protein 4specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Retinol binding protein 4 captured in plate.

Get higher sensitivity in only 90 minutes with Human Retinol Binding Protein 4 ELISA Kit (ab196264) from our SimpleStep ELISA® range.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.5" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RBP4 or retinol-binding protein 4 is a carrier protein that is essential for the transport of retinol or vitamin A in the bloodstream. Also known by the names RBP or plasma retinol-binding protein this protein has a molecular weight of approximately 21 kDa. RBP4 is mainly expressed in the liver and secreted into the blood where it binds to retinol and protects it from oxidation. The structure of RBP4 allows it to fit into binding pockets facilitating the transport of retinol to peripheral tissues.
Biological function summary

RBP4 plays a role as a transporter of vitamin A from the liver to tissues where it is needed. It forms complexes with transthyretin in the blood which prevents kidney filtration and subsequent loss of retinol. This function ensures the proper delivery and availability of retinol essential for vision cellular differentiation and immune system function. The stability of the retinol-RBP4 complex is important to maintaining sufficient vitamin A levels in the body.

Pathways

RBP4 is significant in the vitamin A metabolism pathway and affects the retinoid signaling pathways. These pathways are critical for processes such as vision and skin health. RBP4 interacts with proteins like lecithin retinol acyltransferase (LRAT) in these pathways which are responsible for esterifying retinol into retinyl esters a storage form of vitamin A. These interactions ensure the efficient transport and storage of vitamin A and that it is accessible when needed by different tissues.

RBP4 is linked to the development of insulin resistance and type 2 diabetes. Elevated RBP4 levels correlate with these conditions and highlight its role in metabolic processes. Studies also show a connection between altered RBP4 levels and obesity with insulin resistance often occurring in conjunction with obesity. The protein is implicated in these metabolic disorders through its interactions with insulin-regulating proteins which influence glucose homeostasis.
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Product protocols

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Target data

Retinol-binding protein that mediates retinol transport in blood plasma (PubMed : 5541771). Delivers retinol from the liver stores to the peripheral tissues (Probable). Transfers the bound all-trans retinol to STRA6, that then facilitates retinol transport across the cell membrane (PubMed : 22665496).
See full target information RBP4
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 10:594 PubMed31953481

2020

Ultrasensitive nano-aptasensor for monitoring retinol binding protein 4 as a biomarker for diabetes prognosis at early stages.

Applications

Unspecified application

Species

Unspecified reactive species

Raheleh Torabi,Hedayatollah Ghourchian

Sport sciences for health 15:551-558 PubMed31885723

2019

Plasma retinol-binding protein-4 and tumor necrosis factor-α are reduced in postmenopausal women after combination of different intensities of circuit resistance training and Zataria supplementation.

Applications

Unspecified application

Species

Unspecified reactive species

Seyed Morteza Tayebi,Ayoub Saeidi,Mohamad Fashi,Seyed Pouya,Abedin Khosravi,Hossein Shirvani,Mehdi Ahmadian,Abderraouf Ben Abderrahman,Anthony C Hackney,Hassane Zouhal

Data in brief 8:536-44 PubMed27508213

2016

Data correlations between gender, cytomegalovirus infection and T cells, NK cells, and soluble immune mediators in elderly humans.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmad Al-Attar,Steven R Presnell,Charlotte A Peterson,D Travis Thomas,Charles T Lutz

Scientific reports 6:19667 PubMed26792617

2016

Urinary Kininogen-1 and Retinol binding protein-4 respond to Acute Kidney Injury: predictors of patient prognosis?

Applications

Unspecified application

Species

Unspecified reactive species

Laura Gonzalez-Calero,Marta Martin-Lorenzo,Angeles Ramos-Barron,Jorge Ruiz-Criado,Aroa S Maroto,Alberto Ortiz,Carlos Gomez-Alamillo,Manuel Arias,Fernando Vivanco,Gloria Alvarez-Llamas

Human molecular genetics 23:4077-85 PubMed24670599

2014

PNPLA3 has retinyl-palmitate lipase activity in human hepatic stellate cells.

Applications

Unspecified application

Species

Unspecified reactive species

Carlo Pirazzi,Luca Valenti,Benedetta Maria Motta,Piero Pingitore,Kristina Hedfalk,Rosellina Margherita Mancina,Maria Antonella Burza,Cesare Indiveri,Yvelise Ferro,Tiziana Montalcini,Cristina Maglio,Paola Dongiovanni,Silvia Fargion,Raffaela Rametta,Arturo Pujia,Linda Andersson,Saswati Ghosal,Malin Levin,Olov Wiklund,Michelina Iacovino,Jan Borén,Stefano Romeo
View all publications
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