Human Retinol binding protein ELISA Kit (RBP)
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(1 Publication)
Human Retinol binding protein ELISA Kit (RBP) is a Competitive ELISA for the measurement of Human Retinol binding protein (RBP) in Human in Biofluids samples.
View Alternative Names
CRBP1, RBP1, Retinol-binding protein 1, Cellular retinol-binding protein, Cellular retinol-binding protein I, CRBP, CRBP-I
- sELISA
Supplier Data
Sandwich ELISA - Human Retinol binding protein ELISA Kit (RBP) (AB137991)
Representative Standard Curve using ab137991.
Reactivity data
Product details
Abcam's Retinol Binding Protein (RBP) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of RBP in plasma and serum.
A Retinol Binding Protein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Retinol Binding Protein is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Retinol Binding Protein captured in plate.
The immunogen for the RBP-capture antibody is total RBP and thus the capture antibody should detect both RBP1 and RPB4, among others.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Properties and storage information
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The RBP4 plays an essential role in the regulation of vitamin A homeostasis. It forms a complex with transthyretin which prevents the loss of RBP-retinol in the kidney by inhibiting glomerular filtration. This interaction stabilizes the entire structure allowing efficient retinol delivery to target cells. The tight regulation and functioning of this complex are important for numerous bodily processes that require vitamin A including vision cellular growth and differentiation.
Pathways
The function of RBP4 is significantly associated with the retinol metabolism pathway and the visual cycle pathway. In retinol metabolism it works in tandem with other proteins such as lecithin retinol acyltransferase (LRAT) to control retinol levels in the body. In the visual cycle pathway RBP4 interacts indirectly with visual cycle proteins like opsins to support retinal release from the bloodstream to eye tissues essential for the maintenance of healthy vision.
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Publications (1)
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Cellular physiology and biochemistry : internation 42:594-602 PubMed28954270
2017
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