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AB314379

Human RGMB ELISA Kit

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Human RGMB ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human RGMB in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Repulsive guidance molecule B, DRG11-responsive axonal guidance and outgrowth of neurite, DRAGON, RGMB

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Sandwich ELISA - Human RGMB ELISA Kit (AB314379)
  • sELISA

Supplier Data

Sandwich ELISA - Human RGMB ELISA Kit (AB314379)

Example of standard curve using ab314379. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.21 ng/mL

Range

0.21 - 50 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human RGMB ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human RGMB in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human RGMB coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAMTS13, Aggrecan, Angiotensinogen (SERPINA8), B7- H1 (CD274), BMPR-IA (ALK-3), BMPR-II, Cadherin-11, CD27 (TNFRSF7), CD6, Ck beta 8-1 (CCL23), CNTF, DNAM1 (CD226), CD147 (EMMPRIN), FLRG, Follistatin-like 1, Fractalkine (CX3CL1), Galectin-1, GITR Ligand (TNFSF18), Granulysin (NKG5/LAG-2), IL-1 R3 (IL-1 R Acp), IL-15 R alpha, IL-17E (IL-25), IL32 alpha, CHL-1 (L1CAM-2), LRIG3, LRP-6, MEPE (OF45), Nectin-4, Periostin, Persephin, Renin, ROBO3, S100 A8, Siglec-7 (CD328), Syndecan-3, Thrombospondin 2, Thrombospondin 5 (COMP), Tie-1, ULBP-2 (ALCAN-alpha/N2DL2).

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Recovery

[ { "sample": "Serum", "range": "81 - 123 %", "average": "= 98.86" }, { "sample": "Plasma", "range": "70 - 99 %", "average": "= 83.35" }, { "sample": "Cell culture media", "range": "71 - 77 %", "average": "= 73.81" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314379-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Human RGMB Antibody-coated ELISA Plate", "number":"AB314379-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314379-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human RGMB Protein Standard", "number":"AB314379-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin", "number":"AB314379-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314379-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314379-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human RGMB Detection Antibody", "number":"AB314379-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314379-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DRAGON also known as Repulsive Guidance Molecule b (RGMb) is a glycoprotein involved in the regulation of signaling pathways. It weighs approximately 50 kDa and is widely expressed in various tissues including the central nervous system liver and developing limbs. As a prominent member of the RGM family DRAGON plays a part in critical cellular processes such as growth and differentiation although the detailed mechanical actions require further exploration.
Biological function summary

This protein is significant in iron metabolism and the bone morphogenetic protein (BMP) signaling pathway. It acts not only independently but also as part of a complex with the BMP receptors playing roles in embryogenesis and neurodevelopment. Its binding properties enable it to modulate BMP signals important in processes like bone formation and neural patterning.

Pathways

DRAGON interacts primarily within the BMP signaling pathway and the hepcidin pathway. By coupling with BMPs such as BMP-2 and BMP-6 DRAGON influences processes like cellular growth and apoptosis guide. Its participation in the hepcidin pathway impacts iron homeostasis as it potentially regulates hepcidin an essential hormone produced by the liver that governs iron absorption and distribution in the body. These interactions highlight DRAGON's role in coordinating complex biological activities alongside proteins like hemojuvelin and neogenin.

DRAGON's dysfunction links to conditions like anemia and cancer. Abnormalities in its expression or interaction may result in improper iron metabolism seen in anemia of chronic disease. Further its role in BMP signaling connects it to oncogenic processes contributing to tumor development in cancer. The interaction with other proteins such as BMPs and neogenin suggests that modulation of these pathways could present therapeutic avenues in diseases linked to DRAGON dysregulation.
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Product protocols

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Target data

Member of the repulsive guidance molecule (RGM) family that contributes to the patterning of the developing nervous system (By similarity). Acts as a bone morphogenetic protein (BMP) coreceptor that potentiates BMP signaling (By similarity). Promotes neuronal adhesion (By similarity). May inhibit neurite outgrowth.
See full target information RGM domain family member B
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB96727

Anti-DRAGON antibody

Western blot - Anti-DRAGON antibody (AB96727)

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