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AB243680

Human RHOC ELISA Kit

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Human RHOC ELISA Kit is a single-wash 90-min Simplestep used to quantify Human RHOC with a sensitivity of 140 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

ARH9, ARHC, RHOC, Rho-related GTP-binding protein RhoC, Rho cDNA clone 9, h9

3 Images
Sandwich ELISA - Human RHOC ELISA Kit (AB243680)
  • sELISA

Supplier Data

Sandwich ELISA - Human RHOC ELISA Kit (AB243680)

Representative image of Human RHOC ELISA Kit (ab243680)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human RHOC ELISA Kit Capture Antibody

- 10X Human RHOC Detector Antibody

- 10X Human RHOC ELISA Kit Lyophilised Purified Protein (2 vials)

- Human RHOC ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Human RHOC ELISA Kit (AB243680)
  • sELISA

Supplier Data

Sandwich ELISA - Human RHOC ELISA Kit (AB243680)

Example of human RHOC standard curve in 1X Cell Extraction Buffer PTR.

The RHOC standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human RHOC ELISA Kit (AB243680)
  • sELISA

Supplier Data

Sandwich ELISA - Human RHOC ELISA Kit (AB243680)

Interpolated concentrations of native RHOC in MCF7, HUVEC, SH-SY5Y, mouse NIH/3T3, and rat liver extracts based on a 300 μg/mL extract load for MCF7 and HUVEC samples, 800 μg/mL for SH-SY5Y sample, 400 μg/mL extract load for NIH/3T3 and rat liver samples. The concentrations of RHOC were measured in duplicate and interpolated from the RHOC standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 140 pg/mL

Range

0.78 - 50 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human RHOC ELISA Kit (ab243680) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of RHOC protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human RHOC with 140 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Cell Extract", "replicates": 7, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.3" }, { "reproducibilityType": "Intra", "sample": "Cell Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.3" } ]
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Recovery

[ { "sample": "Cell culture extracts", "range": "96 - 98 %", "average": "= 97" }, { "sample": "Tissue Extracts", "range": "96 - 99 %", "average": "= 97" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab243680-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB243680-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB243680-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB243680-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB243680-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB243680-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB243680-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human RHOC Capture Antibody", "number":"AB243680-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB243680-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human RHOC Lyophilized recombinant Protein", "number":"AB243680-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human RHOC Detector Antibody", "number":"AB243680-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB243680-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB243680-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RHOC also known as ras homolog family member C is a small GTPase with a molecular mass of approximately 22 kDa. This protein part of the Rho family is involved in various cellular functions including motility and mitosis. RHOC is widely expressed in different tissues but shows higher levels in certain tumor cells. Through its role in actin cytoskeleton organization RHOC mediates cellular structure and activity by binding to GDP and GTP molecules thereby controlling cell morphology and movement.
Biological function summary

RHOC influences cell migration invasion and proliferation key processes in cancer progression. It functions within the Rho family of GTPases interacting with other proteins like ROCK and mDia to regulate cytoskeletal dynamics. RHOC does not operate alone; it works as part of signaling complexes that affect cellular mechanisms such as adhesion and vesicle trafficking. Its activity modulates the balance between cell stability and movement making it significant in tissue remodeling and repair.

Pathways

RHOC plays pivotal roles in the Rho/ROCK and PI3K/Akt signaling pathways. Through these pathways it interacts with related GTPases like RHOA and RAC1. In the Rho/ROCK pathway RHOC influences actin cytoskeleton rearrangement impacting cell contractility and motility. Via the PI3K/Akt pathway RHOC is linked to cell survival and growth involving other proteins like PTEN and mTOR highlighting its function in integrating external signals for cellular response.

RHOC is relevant in cancer and cardiovascular diseases. In cancer elevated RHOC expression correlates with increased invasiveness and metastasis particularly in breast and pancreatic cancers. This association involves proteins such as integrins and cadherins which aid in cell adhesion and migration. RHOC is also implicated in cardiovascular disorders where abnormal cell migration and proliferation contribute to atherosclerosis. Understanding these interactions assists in potential targeting of RHOC for therapeutic purposes.
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Product protocols

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Target data

Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Regulates apical junction formation in bronchial epithelial cells.
See full target information RHOC
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websiteProtocolBooklet
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