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AB325724

Human Ribonuclease 3 (ECP) ELISA Kit, Chemiluminescent

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Human Ribonuclease 3 (ECP) ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Ribonuclease 3 (ECP) with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 2.47 - 5400 pg/ml with a sensitivity of 0.36 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

ECP, RNS3, RNASE3, Eosinophil cationic protein, Ribonuclease 3, RNase 3

7 Images
Sandwich ELISA - Human Ribonuclease 3 (ECP) ELISA Kit, Chemiluminescent (AB325724)
  • sELISA

Lab

Sandwich ELISA - Human Ribonuclease 3 (ECP) ELISA Kit, Chemiluminescent (AB325724)

Interpolated concentrations of native Ribonuclease 3 in human U937 cell extract sample based on a 4,000 ng/mL extract load. The concentrations of Ribonuclease 3 were measured in duplicate and interpolated from the Ribonuclease 3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Ribonuclease 3 concentration was determined to be 565.9 pg/mL in U937 cell extract sample.

Sandwich ELISA - Human Ribonuclease 3 (ECP) ELISA Kit, Chemiluminescent (AB325724)
  • sELISA

Lab

Sandwich ELISA - Human Ribonuclease 3 (ECP) ELISA Kit, Chemiluminescent (AB325724)

Dynamic range comparison between the Chemiluminescent Human Ribonuclease 3 SimpleStep ELISA, colorimetric Human Ribonuclease 3 SimpleStep ELISA (ab279415), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human Ribonuclease 3 (ECP) ELISA Kit, Chemiluminescent (AB325724)
  • sELISA

Lab

Sandwich ELISA - Human Ribonuclease 3 (ECP) ELISA Kit, Chemiluminescent (AB325724)

Interpolated concentrations of native Ribonuclease 3 in human cell culture supernatant samples. The concentrations of Ribonuclease 3 were measured in duplicates, interpolated from the Ribonuclease 3 standard curves and corrected for sample dilution. Undiluted samples are as follows : PMBC stimulated 12.5% and PBMC unstimulated 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Ribonuclease 3 concentration was determined to be 2,787 pg/mL in stimulated PBMC supernatant and 1,335 pg/mL in unstimulated PBMC supernatant.

Sandwich ELISA - Human Ribonuclease 3 (ECP) ELISA Kit, Chemiluminescent (AB325724)
  • sELISA

Lab

Sandwich ELISA - Human Ribonuclease 3 (ECP) ELISA Kit, Chemiluminescent (AB325724)

Interpolated concentrations of native Ribonuclease 3 in human serum and plasma samples. The concentrations of Ribonuclease 3 were measured in duplicates, interpolated from the Ribonuclease 3 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 5%, plasma (citrate) 12.5%, plasma (EDTA) 12.5%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Ribonuclease 3 concentration was determined to be 5,486 pg/mL in serum, 4,124 pg/mL in plasma (citrate), 3,493 pg/mL in plasma (EDTA), and 8,780 pg/mL in plasma (heparin).

Sandwich ELISA - Human Ribonuclease 3 (ECP) ELISA Kit, Chemiluminescent (AB325724)
  • sELISA

Lab

Sandwich ELISA - Human Ribonuclease 3 (ECP) ELISA Kit, Chemiluminescent (AB325724)

Serum from ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Ribonuclease 3 concentration was determined to be 8,162 pg/mL with a range of 3,304 – 11,272 pg/mL.

Sandwich ELISA - Human Ribonuclease 3 (ECP) ELISA Kit, Chemiluminescent (AB325724)
  • sELISA

Lab

Sandwich ELISA - Human Ribonuclease 3 (ECP) ELISA Kit, Chemiluminescent (AB325724)

Standard curve comparison between the Chemiluminescent Human Ribonuclease 3 SimpleStep ELISA and colorimetric Human Ribonuclease 3 SimpleStep ELISA (ab279415) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Ribonuclease 3 (ECP) ELISA Kit, Chemiluminescent (AB325724)
  • sELISA

Lab

Sandwich ELISA - Human Ribonuclease 3 (ECP) ELISA Kit, Chemiluminescent (AB325724)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Cell Extract

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.36 pg/mL

Range

2.47 - 5400 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Ribonuclease 3 (ECP) ELISA Kit, Chemiluminescent (ab325724) is a rapid single-wash sandwich 55-min ELISA to measure Human Ribonuclease 3 (ECP) in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell Culture Supernatant, Cell Extract with a sensitivity of 0.36 pg/mL.

How the assay works

Human Ribonuclease 3 (ECP) ELISA Kit, Chemiluminescent (ab325724) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Ribonuclease 3 (ECP) (ab325724) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-RNASE3 antibody [EPR23342-100] - BSA and Aside free (Capture) ab281533
- Anti-RNASE3 antibody [EPR23342-36] - BSA and Aside free (Detector) ab281557

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.6" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.8" } ]
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Recovery

[ { "sample": "Serum", "range": "94 - 109 %", "average": "= 101" }, { "sample": "Citrate plasma", "range": "86 - 91 %", "average": "= 88" }, { "sample": "EDTA Plasma", "range": "97 - 104 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "91 - 94 %", "average": "= 93" }, { "sample": "Cell culture supernatant", "range": "93 - 103 %", "average": "= 98" }, { "sample": "Cell Lysate", "range": "102 - 110 %", "average": "= 106" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab325724-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Human Ribonuclease 3 (ECP) Detector Antibody", "number":"AB325724-CMP02", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB325724-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Ribonuclease 3 (ECP) Lyophilized Recombinant Protein", "number":"AB325724-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB325724-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB325724-CMP05", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB325724-CMP09", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB325724-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB325724-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325724-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Ribonuclease 3 (ECP) Capture Antibody", "number":"AB325724-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB325724-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.
See full target information RNASE3
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com