JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB279415

Human Ribonuclease 3 ELISA Kit (ECP)

Be the first to review this product! Submit a review

|

(1 Publication)

Human Ribonuclease 3 ELISA Kit (ECP) is a single-wash 90-min Simplestep used to quantify Human Ribonuclease 3 (ECP) with a sensitivity of 1.265 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

ECP, RNS3, RNASE3, Eosinophil cationic protein, Ribonuclease 3, RNase 3

6 Images
ELISA - Human Ribonuclease 3 ELISA Kit (ECP) (AB279415)
  • ELISA

Supplier Data

ELISA - Human Ribonuclease 3 ELISA Kit (ECP) (AB279415)
Sandwich ELISA - Human Ribonuclease 3 ELISA Kit (ECP) (AB279415)
  • sELISA

Supplier Data

Sandwich ELISA - Human Ribonuclease 3 ELISA Kit (ECP) (AB279415)

Interpolated concentrations of native Ribonuclease 3 in human serum and plasma samples.

The concentrations of Ribonuclease 3 were measured in duplicates, interpolated from the Ribonuclease 3 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 5%, plasma (citrate) 12.5%, plasma (EDTA) 12.5%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Ribonuclease 3 concentration was determined to be 5,486 pg/mL in serum, 4,124 pg/mL in plasma (citrate), 3,493 pg/mL in plasma (EDTA), and 8,780 pg/mL in plasma (heparin).

Sandwich ELISA - Human Ribonuclease 3 ELISA Kit (ECP) (AB279415)
  • sELISA

Supplier Data

Sandwich ELISA - Human Ribonuclease 3 ELISA Kit (ECP) (AB279415)

Interpolated concentrations of native Ribonuclease 3 in human cell culture supernatant samples.

he concentrations of Ribonuclease 3 were measured in duplicates, interpolated from the Ribonuclease 3 standard curves and corrected for sample dilution. Undiluted samples are as follows : PMBC stimulated 12.5% and PBMC unstimulated 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Ribonuclease 3 concentration was determined to be 2,787 pg/mL in PBMC stimulated and 1,335 pg/mL in PBMC unstimulated.

Sandwich ELISA - Human Ribonuclease 3 ELISA Kit (ECP) (AB279415)
  • sELISA

Supplier Data

Sandwich ELISA - Human Ribonuclease 3 ELISA Kit (ECP) (AB279415)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Ribonuclease 3 concentration was determined to be 8,162 pg/mL with a range of 3,304 – 11,272 pg/mL.

Sandwich ELISA - Human Ribonuclease 3 ELISA Kit (ECP) (AB279415)
  • sELISA

Supplier Data

Sandwich ELISA - Human Ribonuclease 3 ELISA Kit (ECP) (AB279415)

Interpolated concentrations of native Ribonuclease 3 in human U937 cell extract sample based on a 4,000 ng/mL extract load.

The concentrations of Ribonuclease 3 were measured in duplicate and interpolated from the Ribonuclease 3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Ribonuclease 3 concentration was determined to be 565.9 pg/mL in U937 cell extract sample.

Sandwich ELISA - Human Ribonuclease 3 ELISA Kit (ECP) (AB279415)
  • sELISA

Supplier Data

Sandwich ELISA - Human Ribonuclease 3 ELISA Kit (ECP) (AB279415)

Example of human Ribonuclease 3 standard curve in Sample Diluent NS.

The Ribonuclease 3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.265 pg/mL

Range

9.375 - 600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human Ribonuclease 3 ELISA Kit (ECP) ab279415 is a rapid single-wash 90-min Sandwich ELISA to measure Human Ribonuclease 3 (ECP)in cell culture supernatant, cell lysate, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 1.265 pg/mL.

How the assay works

Human Ribonuclease 3 (ECP)SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Ribonuclease 3 ELISA Kit (ECP) ab279415 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Ribonuclease 3, also known as Eosinophil Cationic Protein (ECP) is part of the Ribonuclease A family and is able to degrade RNA. Ribonuclease 3 is expressed by the eosinophils and secreted into the blood stream. Serum and plasma levels of Ribonuclease 3 increase in response to inflammatory diseases. Ribonuclease 3 is measured diagnostically to diagnose allergic disorders and other conditions connected with activated eosinophils.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.6" } ]
style
left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "93 - 103 %", "average": "= 98" }, { "sample": "Serum", "range": "94 - 109 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "97 - 104 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "91 - 94 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "86 - 91 %", "average": "= 88" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279415-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB279415-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB279415-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB279415-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB279415-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB279415-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Ribonuclease 3 (ECP) Lyophilized Recombinant Protein", "number":"AB279415-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Ribonuclease 3 (ECP) Capture Antibody", "number":"AB279415-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB279415-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Ribonuclease 3 (ECP) Detector Antibody", "number":"AB279415-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB279415-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB279415-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ribonuclease 3 commonly known as Eosinophil Cationic Protein (ECP) is an enzyme with ribonuclease activity. Researchers sometimes refer to it as human RNase 3. The protein has a molecular mass of about 18 to 22 kDa and is highly expressed in eosinophils a type of white blood cell primarily involved in allergic responses and parasitic infections. ECP exhibits cytotoxic bactericidal and antiviral activities contributing to its diverse biological functions.
Biological function summary

Ribonuclease 3 plays a significant role in immune defense mechanisms leveraging its arginine-rich domain to interact with negatively charged microbial surfaces. This interaction allows ECP to participate in the destruction of pathogen cell walls. It is not part of a larger complex but interacts with cellular membranes making it an effective tool against a variety of pathogens. Whether acting through enzymatic activity or by disrupting membrane integrity ECP maintains a pivotal role in host defense.

Pathways

ECP influences processes in both innate and adaptive immune responses. It actively participates in the Th2 cell-mediated immune response which is critical for countering parasitic infections. This protein connects with other components like major basic protein and eosinophil peroxidase through these pathways forming an integrated network that boosts immune responses. Its presence in pathways linked to inflammation and immunity highlights its multifunctionality and importance.

ECP levels correlate with conditions such as asthma and eosinophilic esophagitis. In asthma elevated ECP levels indicate eosinophilic inflammation and airway hyperresponsiveness. Researchers have identified a connection between ECP and proteins like interleukin-5 which modulate eosinophil activity. Similarly in eosinophilic esophagitis increased ECP levels indicate ongoing inflammation and tissue damage providing insights into disease progression and severity.
style
left-column

Product protocols

style
left-column

Target data

Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.
See full target information RNASE3
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BMC pulmonary medicine 25:192 PubMed40269819

2025

The role and clinical significance of myeloperoxidase (MPO) and TNF-α in prognostic evaluation of T-COPD.

Applications

Unspecified application

Species

Unspecified reactive species

E Jiang,Yingya Fu,Yalin Wang,Li Ying,Wen Li
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB270204

Human Neutrophil Elastase ELISA Kit

Recombinant|SimpleStep

ELISA - Human Neutrophil Elastase ELISA Kit (AB270204)

  • 0
  • 0
Primary Antibodies

AB190715

Anti-EPX antibody [AHE-1]

  • 4
  • 2
Assay Kits

AB195216

Human IgE ELISA Kit

SimpleStep

ELISA - Human IgE ELISA Kit (AB195216)

  • 0
  • 0
Primary Antibodies

AB207429

Anti-Ribonuclease 3/ECP antibody [EPR20357]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribonuclease 3/ECP antibody [EPR20357] (AB207429)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com