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AB325558

Human RIG-I/DDX58 ELISA Kit - Intracellular

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Human RIG-I/DDX58 ELISA Kit - Intracellular is a single-wash 90-min Simplestep used to quantify Human RIG-I/DDX58 with a sensitivity of 0.29ng/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

-Colormetric sandwich ELISA - 450 nm readout- works on any standard plate reader.

-Different formats for different needs: 10 x 96 plates for bulk orders.

-Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

DDX58, RIGI, Antiviral innate immune response receptor RIG-I, ATP-dependent RNA helicase DDX58, DEAD box protein 58, RIG-I-like receptor 1, RNA sensor RIG-I, Retinoic acid-inducible gene 1 protein, Retinoic acid-inducible gene I protein, RLR-1, RIG-1, RIG-I

2 Images
Sandwich ELISA - Human RIG-I/DDX58 ELISA Kit - Intracellular (AB325558)
  • sELISA

Lab

Sandwich ELISA - Human RIG-I/DDX58 ELISA Kit - Intracellular (AB325558)

Example of human RIG-I/DDX58 standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human RIG-I/DDX58 ELISA Kit - Intracellular (AB325558)
  • sELISA

Lab

Sandwich ELISA - Human RIG-I/DDX58 ELISA Kit - Intracellular (AB325558)

Interpolated concentration of native RIG-I/DDX58 was measured in duplicate at different sample concentrations. Undiluted samples are 62.5 µg/mL treated THP-1 cell extract (treatment of 50 nM PMA for 24h followed by 5 ug/mL LPS for 24h). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Cell Extract

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.29 ng/mL

Range

1.25 - 80 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human RIG-I/DDX58 ELISA Kit - Intracellular (ab325558) is a rapid single-wash sandwich 90-min ELISA to measure Human RIG-I/DDX58 in Cell Extract. This SimpleStep sensitivity is 0.29 ng/ml.

How the assay works

Human RIG-I/DDX58 ELISA Kit - Intracellular (ab325558) SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human RIG-I/DDX58 (ab325558) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.6" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.5" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "80 - 83 %", "average": "= 81" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Innate immune receptor that senses cytoplasmic viral nucleic acids and activates a downstream signaling cascade leading to the production of type I interferons and pro-inflammatory cytokines (PubMed : 15208624, PubMed : 15708988, PubMed : 16125763, PubMed : 16127453, PubMed : 16153868, PubMed : 17190814, PubMed : 18636086, PubMed : 19122199, PubMed : 19211564, PubMed : 24366338, PubMed : 28469175, PubMed : 29117565, PubMed : 31006531, PubMed : 34935440, PubMed : 35263596, PubMed : 36793726). Forms a ribonucleoprotein complex with viral RNAs on which it homooligomerizes to form filaments (PubMed : 15208624, PubMed : 15708988). The homooligomerization allows the recruitment of RNF135 an E3 ubiquitin-protein ligase that activates and amplifies the RIG-I-mediated antiviral signaling in an RNA length-dependent manner through ubiquitination-dependent and -independent mechanisms (PubMed : 28469175, PubMed : 31006531). Upon activation, associates with mitochondria antiviral signaling protein (MAVS/IPS1) that activates the IKK-related kinases TBK1 and IKBKE which in turn phosphorylate the interferon regulatory factors IRF3 and IRF7, activating transcription of antiviral immunological genes including the IFN-alpha and IFN-beta interferons (PubMed : 28469175, PubMed : 31006531). Ligands include 5'-triphosphorylated ssRNAs and dsRNAs but also short dsRNAs (<1 kb in length) (PubMed : 15208624, PubMed : 15708988, PubMed : 19576794, PubMed : 19609254, PubMed : 21742966). In addition to the 5'-triphosphate moiety, blunt-end base pairing at the 5'-end of the RNA is very essential (PubMed : 15208624, PubMed : 15708988, PubMed : 19576794, PubMed : 19609254, PubMed : 21742966). Overhangs at the non-triphosphorylated end of the dsRNA RNA have no major impact on its activity (PubMed : 15208624, PubMed : 15708988, PubMed : 19576794, PubMed : 19609254, PubMed : 21742966). A 3'overhang at the 5'triphosphate end decreases and any 5'overhang at the 5' triphosphate end abolishes its activity (PubMed : 15208624, PubMed : 15708988, PubMed : 19576794, PubMed : 19609254, PubMed : 21742966). Detects both positive and negative strand RNA viruses including members of the families Paramyxoviridae : Human respiratory syncytial virus and measles virus (MeV), Rhabdoviridae : vesicular stomatitis virus (VSV), Orthomyxoviridae : influenza A and B virus, Flaviviridae : Japanese encephalitis virus (JEV), hepatitis C virus (HCV), dengue virus (DENV) and west Nile virus (WNV) (PubMed : 21616437, PubMed : 21884169). It also detects rotaviruses and reoviruses (PubMed : 21616437, PubMed : 21884169). Detects and binds to SARS-CoV-2 RNAs which is inhibited by m6A RNA modifications (Ref.74). Also involved in antiviral signaling in response to viruses containing a dsDNA genome such as Epstein-Barr virus (EBV) (PubMed : 19631370). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). May play important roles in granulocyte production and differentiation, bacterial phagocytosis and in the regulation of cell migration.
See full target information RIGI
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websiteProtocolBooklet
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Product promise

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