Human RNase 7 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human RNase 7 in Cell culture media, Urine, Saliva samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Urine, Saliva
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 4.375 - 280 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 2.033 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Exhibits a potent RNase activity (PubMed:12244054, PubMed:12527768, PubMed:17150966). Has broad-spectrum antimicrobial activity against many pathogenic microorganisms including uropathogenic E.coli (UPEC), and remarkably potent activity (lethal dose of 90% < 30 nM) against a vancomycin resistant Enterococcus faecium (PubMed:12244054, PubMed:12527768, PubMed:17150966, PubMed:25075772, PubMed:33818125). Causes loss of bacterial membrane integrity (PubMed:17150966). Probably contributes to urinary tract sterility (PubMed:25075772). Bactericidal activity is independent of RNase activity (PubMed:17150966).
Alternative names
UNQ2516/PRO6006, RNASE7, Ribonuclease 7, RNase 7, Skin-derived antimicrobial protein 2, SAP-2
What's included?
Human RNase 7 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human RNase 7 in Cell culture media, Urine, Saliva samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Urine, Saliva
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 4.375 - 280 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 2.033 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Urine | n 8 | C.V. = 10.9 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Urine | n 3 | C.V. = 3.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture media | Average % = 103 | Range 86 - 115 % |
Sample type Urine | Average % = 117 | Range 113 - 119 % |
Sample type Saliva | Average % = 85 | Range 84 - 86 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human RNase 7 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of RNase 7 protein in Cell culture media, Urine, Saliva. Quantitate Human RNase 7 with 2.033 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibodyanalyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Singlewash protocol reduces assay time to 90 minutes or less.
- High sensitivity, specificity and reproducibility from superior antibodies.
- Fully validated in biological samples.
- 96wells plate breakable into 12 x 8 wells strips, available in 10pack (10 x 96well plates).
- Also available in a fully validated 384well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Sandwich ELISA - Human RNase 7 ELISA Kit (ab324130)
Interpolated concentration of native RNase 7 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 25% urine. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human RNase 7 ELISA Kit (ab324130)
Example of human RNase 7 standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human RNase 7 ELISA Kit (ab324130)
Interpolated concentration of native RNase 7 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: urine 25% and saliva 20%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human RNase 7 ELISA Kit (ab324130)
Example of human RNase 7 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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