AB324130

Human RNase 7 ELISA Kit

Recombinant
SimpleStep
What is this?

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Human RNase 7 ELISA Kit is a Sandwich ELISA for the measurement of Human RNase 7 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

UNQ2516/PRO6006, RNASE7, Ribonuclease 7, RNase 7, Skin-derived antimicrobial protein 2, SAP-2

4 Images

Sandwich ELISA - Human RNase 7 ELISA Kit (AB324130)

sELISA

Supplier Data

Sandwich ELISA - Human RNase 7 ELISA Kit (AB324130)

Interpolated concentration of native RNase 7 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 25% urine. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human RNase 7 ELISA Kit (AB324130)

Interpolated concentration of native RNase 7 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : urine 25% and saliva 20%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human RNase 7 ELISA Kit (AB324130)

Example of human RNase 7 standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human RNase 7 ELISA Kit (AB324130)

Example of human RNase 7 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Urine, Saliva

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.033 pg/mL

Range

4.375 - 280 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human RNase 7 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of RNase 7 protein in Cell culture media, Urine, Saliva. Quantitate Human RNase 7 with 2.033 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibodyanalyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

Singlewash protocol reduces assay time to 90 minutes or less.
High sensitivity, specificity and reproducibility from superior antibodies.
Fully validated in biological samples.
96wells plate breakable into 12 x 8 wells strips, available in 10pack (10 x 96well plates).
Also available in a fully validated 384well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Urine", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 10.9" }, { "reproducibilityType": "Inter", "sample": "Urine", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.3" } ]

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Recovery

[ { "sample": "Cell culture media", "range": "86 - 115 %", "average": "= 103" }, { "sample": "Urine", "range": "113 - 119 %", "average": "= 117" }, { "sample": "Saliva", "range": "84 - 86 %", "average": "= 85" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Exhibits a potent RNase activity (PubMed : 12244054, PubMed : 12527768, PubMed : 17150966). Has broad-spectrum antimicrobial activity against many pathogenic microorganisms including uropathogenic E.coli (UPEC), and remarkably potent activity (lethal dose of 90% < 30 nM) against a vancomycin resistant Enterococcus faecium (PubMed : 12244054, PubMed : 12527768, PubMed : 17150966, PubMed : 25075772, PubMed : 33818125). Causes loss of bacterial membrane integrity (PubMed : 17150966). Probably contributes to urinary tract sterility (PubMed : 25075772). Bactericidal activity is independent of RNase activity (PubMed : 17150966).

See full target information RNASE7

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websiteProtocolBooklet

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Product promise

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