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AB314380

Human Robo3 ELISA Kit

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Human Robo3 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Robo3 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Roundabout homolog 3, Roundabout-like protein 3, ROBO3

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Sandwich ELISA - Human Robo3 ELISA Kit (AB314380)
  • sELISA

Supplier Data

Sandwich ELISA - Human Robo3 ELISA Kit (AB314380)

Example of standard curve using ab314380. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.06 ng/mL

Range

0.06 - 15 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Robo3 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Robo3 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Robo3 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAMTS13, Aggrecan, Angiotensinogen, B7-H1, BMPR-IA, BMPR-II, Cadherin-11, CD27, CD6, Ck beta 8-1, CNTF, DNAM-1, CD147, FLRG, Follistatin-like 1, Fractalkine, Galectin-1, GITR Ligand, Granulysin, IL-1 R3, IL-15 R alpha, IL-17E, IL-32 alpha, CHL-1, LRIG3, LRP-6, MEPE, Nectin-4, Periostin, Persephin, Renin, RGM-B, S100 A8, Siglec-7, Syndecan-3, Thrombospondin 2, Thrombospondin 5, Tie-1, ULBP-2.

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Recovery

[ { "sample": "Serum", "range": "69 - 76 %", "average": "= 72.36" }, { "sample": "Plasma", "range": "69 - 79 %", "average": "= 72.51" }, { "sample": "Cell culture media", "range": "69 - 83 %", "average": "= 76.27" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Robo3 also known as Roundabout homolog 3 or Rig1 is a receptor protein with a mass of approximately 117 kDa. It is expressed in the developing nervous system especially in commissural neurons of the spinal cord. Mechanically Robo3 plays a major role in axon guidance. It does not bind to Slit proteins like other Robo family members but it inhibits Slit-induced repulsion allowing axons to cross the midline.
Biological function summary

The protein facilitates proper nerve cell navigation. It is essential for enabling axons to reach their correct targets by modulating guidance cues. Robo3 is not known as part of a complex but interacts with other guidance signals to steer commissural axons during neural development. Without its functional expression axons fail to cross the midline leading to improper neural circuit formation.

Pathways

Robo3 interacts with signals central to neural circuit development. It mediates important guidance pathways such as the Netrin-DCC pathway working alongside Netrin-1 to promote axonal growth. Robo3 functions in a pathway with other guidance proteins like Robo1 and DCC to ensure neuronal pathfinding is achieved coordinating the switch from attraction to repulsion after midline crossing.

Robo3's malfunction is linked to horizontal gaze palsy with progressive scoliosis (HGPPS) a rare genetic condition affecting eye movements and spinal curvature. Misregulation of related proteins like L1CAM exacerbates navigational errors in axon guidance contributing to the disorder's characteristics. These insights underline the significance of Robo3 in maintaining normal neurological function and its implications in neurodevelopmental conditions.
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Product protocols

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Target data

Receptor involved in axon guidance during development (PubMed : 15105459). Acts as a multifunctional regulator of pathfinding that simultaneously mediates NELL2 repulsion, inhibits SLIT repulsion, and facilitates Netrin-1/NTN1 attraction. In spinal cord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1/ROBO2. Binding OF NELL2 to the receptor ROBO3 promotes oligomerization of ROBO3, resulting in the repulsion of commissural axons in the midline. ROBO3 also indirectly boosts axon attraction to NTN1 without interacting with NTN1 itself (By similarity).
See full target information ROBO3
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB229722

Anti-Robo3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo3 antibody (AB229722)

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