Human ROBO4 ELISA Kit
- Recombinant
- SimpleStep
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Human ROBO4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ROBO4 with a sensitivity of 4.56 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
UNQ421/PRO3674, ROBO4, Roundabout homolog 4, Magic roundabout
- sELISA
Supplier Data
Sandwich ELISA - Human ROBO4 ELISA Kit (AB282879)
Interpolated concentrations of native ROBO4 in human cell culture supernatant samples.
The concentrations of ROBO4 were measured in duplicates, interpolated from the ROBO4 standard curves and corrected for sample dilution. Undiluted samples are as follows : HUVEC supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ROBO4 concentration was determined to be 4684.82 pg/mL in HUVEC cell culture supernatant.
- sELISA
Supplier Data
Sandwich ELISA - Human ROBO4 ELISA Kit (AB282879)
Interpolated concentrations of native ROBO4 in human serum, plasma and cell culture supernatant samples.
The concentrations of ROBO4 were measured in duplicates, interpolated from the ROBO4 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1%, plasma (citrate) 1%, plasma (EDTA) 1% plasma (heparin) 1% and HUVEC supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ROBO4 concentration was determined to be 136.18 ng/mL in serum, 127.46 ng/mL in plasma (citrate), 104.05 ng/mL in plasma (EDTA), and 110.25 ng/mL in plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Human ROBO4 ELISA Kit (AB282879)
0.5 % serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ROBO4 concentration was determined to be 137.64 ng/mL with a range of 74.01 – 211.08 ng/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human ROBO4 ELISA Kit (AB282879)
Example of human ROBO4 standard curve in Sample Diluent NS.
The ROBO4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Complementary Products
Reactivity data
Product details
Human ROBO4 ELISA kit (ab282879) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human ROBO4 protein in human serum, plasma, and supernatants. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ROBO4 with 4.56 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Human ROBO4 (Roundabout homolog 4) is a receptor SLIT2 among Slit family of proteins. Human ROBO4 is involved in the maintenance of endothelial barrier organization and function. Studies suggest ROBO4 is involved in angiogenesis and vascular patterning. ROBO4 may mediate the inhibition of primary endothelial cell migration by Slit proteins.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Robo4 stabilizes the vascular network interacting actively with the guidance molecule Slit proteins. It is not part of a larger protein complex but engages directly with these ligands to influence endothelial cell migration and vascular permeability. Its role is significant in maintaining the structural integrity of blood vessels ensuring the appropriate cellular responses important for proper vascular function.
Pathways
Robo4 engages in the Slit-Robo signaling pathway that is essential for vascular development and remodeling. This pathway helps inhibit excessive endothelial migration and angiogenesis. Robo4 interacts with proteins like Slit2 which binds to it as a ligand to mediate these effects. Through this pathway Robo4 performs an important function in processes such as vascular homeostasis and angiogenesis regulation.
Product protocols
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Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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