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AB267606

Human ROR1 ELISA Kit

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Human ROR1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human ROR1 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

NTRKR1, ROR1, Inactive tyrosine-protein kinase transmembrane receptor ROR1

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Sandwich ELISA - Human ROR1 ELISA Kit (AB267606)
  • sELISA

Supplier Data

Sandwich ELISA - Human ROR1 ELISA Kit (AB267606)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.082 ng/mL

Range

0.819 - 200 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human ROR1 ELISA Kit is designed for the quantitative determination of ROR1 in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for human ROR1 coated on a 96-well plate. Standards and samples are pipetted into the wells and ROR1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human ROR1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ROR1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "70 - 78 %", "average": "= 73.36" }, { "sample": "Plasma", "range": "68 - 79 %", "average": "= 71.44" }, { "sample": "Cell culture media", "range": "69 - 81 %", "average": "= 72.05" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267606-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267606-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267606-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human ROR1 coated Microplate (12 x 8 wells)", "number":"AB267606-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB267606-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB267606-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267606-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human ROR1 Standard (Lyophilized)", "number":"AB267606-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human ROR1 Detection Antibody", "number":"AB267606-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Has very low kinase activity in vitro and is unlikely to function as a tyrosine kinase in vivo (PubMed : 25029443). Receptor for ligand WNT5A which activate downstream NFkB signaling pathway and may result in the inhibition of WNT3A-mediated signaling (PubMed : 25029443, PubMed : 27162350). In inner ear, crucial for spiral ganglion neurons to innervate auditory hair cells (PubMed : 27162350). Via IGFBP5 ligand, forms a complex with ERBB2 to enhance CREB oncogenic signaling (PubMed : 36949068).
See full target information ROR1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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