JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB289641

Human ROR1 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human ROR1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ROR1 with a sensitivity of 2.248 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

NTRKR1, ROR1, Inactive tyrosine-protein kinase transmembrane receptor ROR1

2 Images
Sandwich ELISA - Human ROR1 ELISA Kit (AB289641)
  • sELISA

Supplier Data

Sandwich ELISA - Human ROR1 ELISA Kit (AB289641)

Interpolated concentrations of human ROR1 in U-2 OS extract, A431 extract, and CACO2 extract.

Interpolated concentration of native ROR1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : U-2 OS extract 200 μg/mL, A431 extract 200 μg/mL, and CACO2 extract 200 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human ROR1 ELISA Kit (AB289641)
  • sELISA

Supplier Data

Sandwich ELISA - Human ROR1 ELISA Kit (AB289641)

Example of human ROR1 standard curve in 1X Cell Extraction Buffer PTR.

Example of human ROR1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.248 pg/mL

Range

12.5 - 800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Primary Antibodies

AB280197

Anti-ROR1 antibody [EPR24451-141]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-ROR1 antibody [EPR24451-141] (AB280197)

  • 0
  • 0
Proteins & Peptides

AB140807

Recombinant human ROR1 protein

Functional Studies - Recombinant human ROR1 protein (AB140807)

  • 0
  • 0
Assay Kits

AB279909

Phosphotyrosine ROR2 ELISA Kit

Sandwich ELISA - Phosphotyrosine ROR2 ELISA Kit (AB279909)

  • 0
  • 0
Assay Kits

AB264616

Human CTLA-4 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CTLA-4 ELISA Kit (AB264616)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human ROR1 ELISA Kit (ab289641) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human ROR1 in cell extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ROR1 with 2.248 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.5" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.7" } ]
style
left-column

Recovery

[ { "sample": "Cell Lysate", "range": "109 - 116 %", "average": "= 113" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab289641-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB289641-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB289641-CMP12", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB289641-CMP15", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB289641-CMP14", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human ROR1 Lyophilized Recombinant Protein", "number":"AB289641-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human ROR1 Capture Antibody", "number":"AB289641-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB289641-CMP03", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB289641-CMP13", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human ROR1 Detector Antibody", "number":"AB289641-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB289641-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB289641-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB289641-CMP11", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Has very low kinase activity in vitro and is unlikely to function as a tyrosine kinase in vivo (PubMed : 25029443). Receptor for ligand WNT5A which activate downstream NFkB signaling pathway and may result in the inhibition of WNT3A-mediated signaling (PubMed : 25029443, PubMed : 27162350). In inner ear, crucial for spiral ganglion neurons to innervate auditory hair cells (PubMed : 27162350). Via IGFBP5 ligand, forms a complex with ERBB2 to enhance CREB oncogenic signaling (PubMed : 36949068).
See full target information ROR1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com