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AB314381

Human RSPO2 ELISA Kit

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Human RSPO2 ELISA Kit is a Sandwich ELISA for the measurement of Human RSPO2 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

UNQ9384/PRO34209, RSPO2, R-spondin-2, Roof plate-specific spondin-2, hRspo2

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Sandwich ELISA - Human RSPO2 ELISA Kit (AB314381)
  • sELISA

Supplier Data

Sandwich ELISA - Human RSPO2 ELISA Kit (AB314381)

Example of standard curve using ab314381. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.55 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human RSPO2 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human RSPO2 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human RSPO2 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAM22, Arylsulfatase B/ARSB, B3GNT2, Carbonic Anhydrase VB, Caspase 7, Caspase 8, Integrin alpha M/CD11b, SIRP gamma/CD172g, CD39L2/ENTPD6, CD39L4/ENTPD5, Integrin alpha 2/CD49b, CD7, CEACAM-3, Carboxypeptidase E/CPE, FABP6, FAM3C, GDF-3, GSTM1, Kallikrein 11, Kallikrein 12, Kremen-2, OSCAR, PTP1B, Reg3A, S100A13, Semaphorin 4C, Sirtuin 1/SIRT1, SMPD1, Sortilin, SPINK1, Stabilin-2, SULT2A1, Transcobalamin II, THSD1, TrkA, UCH-L3, VAPA, vWF-A2, Wnt-4.

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Recovery

[ { "sample": "Serum", "range": "69 - 74 %", "average": "= 71.13" }, { "sample": "Plasma", "range": "67 - 86 %", "average": "= 75.25" }, { "sample": "Cell culture media", "range": "81 - 87 %", "average": "= 83.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314381-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314381-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314381-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314381-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human RSPO2 Antibody-coated ELISA Plate", "number":"AB314381-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human RSPO2 Detection Antibody", "number":"AB314381-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB314381-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB314381-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human RSPO2 Protein Standard", "number":"AB314381-CMP11", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB314381-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RSPO2 also known as R-spondin-2 is a secreted protein weighing approximately 35 kDa. It acts mechanically by potentiating the Wnt signaling pathway. This protein is part of the R-spondin family and contains a signal peptide followed by two furin-like repeat domains a thrombospondin type 1 domain and a C-terminal region. RSPO2 is expressed in various tissues with notable expression in the lung muscle and reproductive organs.
Biological function summary

The protein enhances Wnt signaling which is important for the regulation of cellular processes including proliferation and differentiation. RSPO2 interacts with LGR receptors and is essential in modulating tissue development and homeostasis. The protein forms part of a complex with ZNRF3 triggering removal of negative regulators from the cell surface. This fine regulation allows RSPO2 to play roles in skeletal and organ development.

Pathways

RSPO2 actively participates in the canonical Wnt/β-catenin signaling and non-canonical pathways. Within the Wnt/β-catenin pathway RSPO2 collaborates with LRP6 and Frizzled receptors to stabilize β-catenin which then translocates to the nucleus to influence gene expression. In pathways regulating tissue homeostasis RSPO2 works with RNF43 to control ZNRF3 activity affecting how many receptors are available for Wnt signaling.

RSPO2 shows involvement with craniofacial development disorders and certain cancers. Mutations or dysregulation of RSPO2 and its partners such as LRP6 can lead to craniofacial deformities stemming from improper cell signaling during embryonic development. In cancer RSPO2 may contribute to tumor growth due to its role in enhancing Wnt signaling which is often activated aberrantly in tumor cells. The interplay with ZNRF3 in cancer illustrates its wider impact on tissue overgrowth and malignant transformation.
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Product protocols

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Target data

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway (PubMed : 21727895, PubMed : 21909076, PubMed : 22615920). During embryonic development, plays a crucial role in limb specification, amplifying the Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3, hence governing the number of limbs an embryo should form (PubMed : 29769720).
See full target information RSPO2
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Product promise

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