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AB108767

Human Anti-Rubella virus IgG ELISA Kit

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(1 Publication)

Human Anti-Rubella virus IgG ELISA Kit is an indirect ELISA for the qualitative detection of IgG class antibodies against Rubella virus in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
- Cut-off, positive and negative controls included
1 Images
Indirect ELISA - Human Anti-Rubella virus IgG ELISA Kit (AB108767)
  • I-ELISA

Unknown

Indirect ELISA - Human Anti-Rubella virus IgG ELISA Kit (AB108767)

Representative Standard Curve using ab108767

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Indirect

Sensitivity

= 0.45 U/mL

Range

10 - 100 U/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's anti-Rubella virus IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Rubella virus in Human serum and plasma.

A 96-well plate has been precoated with Rubella virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Rubella virus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Rubella virus IgG sample captured in plate.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Standard C", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.6" }, { "reproducibilityType": "Inter", "sample": "Standard D", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.3" }, { "reproducibilityType": "Inter", "sample": "Standard B", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.6" }, { "reproducibilityType": "Intra", "sample": "Standard B", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.9" }, { "reproducibilityType": "Intra", "sample": "Standard C", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" }, { "reproducibilityType": "Intra", "sample": "Standard D", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108767-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"Rubella virus anti-IgG HRP Conjugate", "number":"AB108767-CMP02", "productcode":"" }, { "size":"1 x 2 mL", "name":"Rubella virus IgG Standard D – 100 U/mL", "number":"AB108767-CMP07", "productcode":"" }, { "size":"1 x 2 mL", "name":"Rubella virus IgG Standard C – 50 U/mL", "number":"AB108767-CMP06", "productcode":"" }, { "size":"1 x 2 mL", "name":"Rubella virus IgG Standard B – 10 U/mL", "number":"AB108767-CMP05", "productcode":"" }, { "size":"1 x 2 mL", "name":"Rubella virus IgG Standard A – 0 U/mL", "number":"AB108767-CMP04", "productcode":"" }, { "size":"1 x 100 mL", "name":"IgG Sample Diluent", "number":"AB108767-CMP01", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Washing Solution", "number":"AB108767-CMP11", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108767-CMP12", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108767-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover foil", "number":"AB108767-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB108767-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Rubella virus Coated Microplate (12 x 8 wells)", "number":"AB108767-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rubella Virus IgG also known as anti-rubella IgG represents a group of antibodies directed against the rubella virus. These antibodies are important for identifying and assessing immune response to the rubella virus infection. They are predominantly found in the blood serum of individuals who have been vaccinated against rubella or who have had a rubella virus infection. Anti-rubella IgG antibodies offer reliable evidence of immunity and long-term protection against the virus. Typically no standard mass applies to IgG antibodies as they can vary slightly between individuals. These antibodies are mainly expressed in response to an immune challenge specifically from the rubella virus.
Biological function summary

Anti-rubella IgG antibodies play a significant role in neutralizing the rubella virus and preventing its replication. These antibodies do not act as part of a larger protein complex but instead bind directly to antigens on the virus surface. If the antibodies demonstrate high avidity they indicate a mature immune response which could be significant in determining the timing of rubella infection. A high avidity index often suggests past infection or vaccination providing valuable information about the rubella immunity status. Therefore measuring the avidity of anti-rubella IgG can help distinguish between recent and past infections.

Pathways

The immune response pathways involve these antibodies as a part of the adaptive immune response. Key pathways include the humoral immune response where B cells produce specific antibodies against rubella after recognizing the virus antigens. Other proteins such as CD4+ T-helper cells assist B cells in differentiating into plasma cells that secrete these antibodies. This process strengthens the body's defense mechanisms by equipping it with memory against future rubella exposures.

Rubella virus IgG antibodies are especially pivotal in the context of congenital rubella syndrome (CRS) which can occur when a pregnant woman contracts rubella virus. Detecting these antibodies in pregnant women can assess immunity and risk of CRS. Additionally they might relate to autoimmune disorders where the enhanced immune activity could trigger autoantibody production. Here connections with proteins like rheumatoid factor which is often elevated in autoimmune conditions might be observed reflecting the immune system's broader response dynamics.
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Product protocols

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Target data

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

EBioMedicine 106:105249 PubMed39024898

2024

Sustained chronic inflammation and altered childhood vaccine responses in children exposed to Zika virus.

Applications

Unspecified application

Species

Unspecified reactive species

Suan-Sin Foo,Weiqiang Chen,Tamiris Azamor,Kyle L Jung,Mary Catherine Cambou,Débora Familiar-Macedo,Gielenny M Salem,Ivonne Melano,Myung-Shin Sim,Maria Elisabeth Moreira,Patricia Brasil,Zilton Vasconcelos,Karin Nielsen-Saines,Jae U Jung
View all publications
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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