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AB314383

Human S100 alpha 2/S100A2 ELISA Kit

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Human S100 alpha 2/S100A2 ELISA Kit is a Sandwich ELISA for the measurement of Human S100 alpha 2/S100A2 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

S100L, S100A2, Protein S100-A2, CAN19, Protein S-100L, S100 calcium-binding protein A2

1 Images
Sandwich ELISA - Human S100 alpha 2/S100A2 ELISA Kit (AB314383)
  • sELISA

Supplier Data

Sandwich ELISA - Human S100 alpha 2/S100A2 ELISA Kit (AB314383)

Example of standard curve using ab314383. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.05 ng/mL

Range

0.05 - 10 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB109494

Anti-S100 alpha 2/S100A2 antibody [EPR5392]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha 2/S100A2 antibody [EPR5392] (AB109494)

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Proteins & Peptides

AB104821

Recombinant Human S100 alpha 2/S100A2 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human S100 alpha 2/S100A2 protein (His tag N-Terminus) (AB104821)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human S100 alpha 2/S100A2 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human S100 alpha 2/S100A2 in serum (S100 alpha 2/S100A2 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma (collect plasma using EDTA as an anticoagulant. Heparin and Citrate are not recommended as anticoagulants for use in this assay) and cell culture supernatants. This assay employs an antibody specific for S100 alpha 2/S100A2 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "69 - 101 %", "average": "= 79.82" }, { "sample": "Plasma", "range": "67 - 74 %", "average": "= 70.84" }, { "sample": "Cell culture media", "range": "83 - 115 %", "average": "= 103.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314383-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin", "number":"AB314383-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human S100 alpha 2/S100A2 Detection Antibody", "number":"AB314383-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314383-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human S100 alpha 2/S100A2 Antibody-coated ELISA Plate", "number":"AB314383-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314383-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314383-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human S100 alpha 2/S100A2 Protein Standard", "number":"AB314383-CMP10", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314383-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

S100 alpha 2 also known as S100A2 or CAN19 is a calcium-binding protein that plays a role in the regulation of cell functions. It's part of the S100 protein family and has a mass of approximately 11 kDa. S100A2 is expressed in a variety of tissues including epidermal cells and lung tissue suggesting its involvement in diverse cellular processes. The presence in these tissues indicates a potential role in both skin biology and respiratory system functions.
Biological function summary

S100A2 influences cell proliferation differentiation and apoptosis. It acts by modulating the activity of target proteins through a calcium-dependent mechanism. Though S100A2 is not known to form a complex with other proteins for its function it interacts with key cell growth regulators affecting cellular responses to external signals. The precise modulation of these processes implicates S100A2 in the maintenance of normal tissue homeostasis and cell cycle regulation.

Pathways

S100A2 participates in signal transduction pathways such as the MAPK/ERK and p53 pathways. In the MAPK/ERK pathway S100A2 regulates cell cycle progression and cellular responses to growth factors. Additionally in the p53 pathway S100A2 plays a role in apoptosis and tumor suppression. S100A2’s function links it to interaction with critical proteins such as p53 influencing both stress response and genomic stability.

S100A2 relates to cancer progression and pulmonary diseases. Altered expression levels of S100A2 have been observed in lung cancer and some skin cancers where it may act as a tumor suppressor. In pulmonary conditions its expression affects tissue responses under pathological states. The interaction of S100A2 with proteins like p53 in these disorders suggests a pivotal role in mediating stress responses and regulating tumor growth providing potential therapeutic targets.
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Product protocols

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Target data

May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes. May also play a role in suppressing tumor cell growth.
See full target information S100A2
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websiteProtocolBooklet
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Product promise

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