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Human S100A12 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human S100A12 in Human in Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum samples.

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Sandwich ELISA - Human S100A12 ELISA Kit (AB213822), expandable thumbnail

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Key facts

Detection method
Colorimetric
Sample types
Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
31.2 - 2000 pg/mL
Assay time
3h 30m
Sensitivity
< 10 pg/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

S100A12 is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its pro-inflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of pro-inflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. Acts as a monocyte and mast cell chemoattractant. Can stimulate mast cell degranulation and activation which generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation. Can inhibit the activity of matrix metalloproteinases; MMP2, MMP3 and MMP9 by chelating Zn(2+) from their active sites. Possesses filariacidal and filariastatic activity. Calcitermin possesses antifungal activity against C.albicans and is also active against E.coli and P.aeruginosa but not L.monocytogenes and S.aureus.

Alternative names

Protein S100-A12, CGRP, Calcium-binding protein in amniotic fluid 1, Calgranulin-C, Extracellular newly identified RAGE-binding protein, Migration inhibitory factor-related protein 6, Neutrophil S100 protein, S100 calcium-binding protein A12, CAAF1, CAGC, EN-RAGE, MRP-6, p6, S100A12

What's included?

1 x 96 Tests
Components
ABC Diluent Buffer
1 x 12 mL
Adhesive Plate Seal
4 x 1 Unit
Anti-Human S100A12 coated Microplate (12 x 8 wells)
1 x 1 Unit
Antibody Diluent Buffer
1 x 12 mL
Avidin-Biotin-Peroxidase Complex (ABC)
1 x 100 µL
Biotinylated S100A12 Antibody
1 x 100 µL
Lyophilized recombinant human S100A12 standard
2 x 1 Vial
Sample Diluent Buffer
1 x 30 mL
TMB Color Developing Agent
1 x 10 mL
TMB Stop Solution
1 x 10 mL
Wash Buffer (25X)
1 x 20 mL

Recommended products

Human S100A12 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human S100A12 in Human in Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum samples.

Key facts

Detection method
Colorimetric
Sample types
Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
31.2 - 2000 pg/mL
Assay time
3h 30m
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
< 10 pg/mL

Precision

Intra assay

Sample
Sample 1
n
16
mean
58 pg/mL
SD
2.32
C.V.
= 4
Sample
Sample 2
n
16
mean
265 pg/mL
SD
20.4
C.V.
= 7.7
Sample
Sample 3
n
16
mean
947 pg/mL
SD
67.23
C.V.
= 7.1

Inter assay

Sample
Sample 3
n
24
mean
855 pg/mL
SD
68.4
C.V.
= 8
Sample
Sample 1
n
24
mean
56 pg/mL
SD
3.08
C.V.
= 5.5
Sample
Sample 2
n
24
mean
247 pg/mL
SD
23.46
C.V.
= 9.5

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

The Human S100A12 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213822) is designed for the quantitative measurement of Human S100A12 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for S100A12 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for S100A12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human S100A12 amount of sample captured in plate.

S100 calcium-binding protein A12 (S100A12) is a protein that in humans is encoded by the S100A12 gene. It is also known as calgranulin C. By direct R-banding FISH, the S100A12 gene is mapped to chromosome 1q21.2-q22. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. And this protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The S100A12 protein also known as Calgranulin C or CGRP (Calcitonin Gene-Related Peptide) has a molecular mass of approximately 10.4 kDa. This protein shows expression primarily in neutrophils but is also present in other types of cells such as epithelial cells and certain cell lines. S100A12 belongs to the S100 protein family which is part of the EF-hand calcium-binding proteins known for playing roles in cell regulation. The protein binds calcium ions which influence its structural conformation.

Biological function summary

S100A12 is important in the inflammatory response and immune system regulation. It acts as a pro-inflammatory mediator attracting leukocytes to sites of inflammation. Furthermore it can bind to receptors on cell surfaces like RAGE (Receptor for Advanced Glycation End Products) which induces signaling cascades that amplify immune responses. S100A12 does not function alone but often forms homodimers or heterodimers with other S100 proteins enhancing its biological activities.

Pathways

S100A12 participates in pathways like the inflammatory response pathway and the RAGE signaling pathway. By interacting with RAGE and possibly other receptors it impacts cellular responses associated with stress injury and microbial infection. This interaction connects S100A12 to other proteins in the same pathways such as NF-kB an important transcription factor involved in inflammation and immune responses.

Associated diseases and disorders

S100A12 has connections with conditions like rheumatoid arthritis and inflammatory bowel disease. Elevated levels of S100A12 often correlate with disease activity and severity suggesting its potential as a biomarker. In rheumatoid arthritis it contributes to the inflammatory environment while in inflammatory bowel disease it associates not only with inflammation but also with proteins like IL-6 and TNF-alpha which are significant cytokines in the pathogenesis of these diseases.

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  • Sandwich ELISA - Human S100A12 ELISA Kit (ab213822), expandable thumbnail

    Sandwich ELISA - Human S100A12 ELISA Kit (ab213822)

    Human S100A12 ELISA Kit (ab213822) Standard Curve.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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