AB213822

Human S100A12 ELISA Kit

Name: Human S100A12 ELISA Kit (ab213822) | Abcam
Brand: Abcam Limited
SKU: AB213822

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(1 Publication)

Human S100A12 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human S100A12 in Human in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

Protein S100-A12, CGRP, Calcium-binding protein in amniotic fluid 1, Calgranulin-C, Extracellular newly identified RAGE-binding protein, Migration inhibitory factor-related protein 6, Neutrophil S100 protein, S100 calcium-binding protein A12, CAAF1, CAGC, EN-RAGE, MRP-6, p6, S100A12

1 Images

Sandwich ELISA - Human S100A12 ELISA Kit (AB213822)

sELISA

Supplier Data

Sandwich ELISA - Human S100A12 ELISA Kit (AB213822)

Human S100A12 ELISA Kit (ab213822) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

31.2 - 2000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

The Human S100A12 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213822) is designed for the quantitative measurement of Human S100A12 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for S100A12 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for S100A12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human S100A12 amount of sample captured in plate.

S100 calcium-binding protein A12 (S100A12) is a protein that in humans is encoded by the S100A12 gene. It is also known as calgranulin C. By direct R-banding FISH, the S100A12 gene is mapped to chromosome 1q21.2-q22. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. And this protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "58 pg/mL", "standardDeviation": "2.32", "coefficientOfVariability": "= 4" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "265 pg/mL", "standardDeviation": "20.4", "coefficientOfVariability": "= 7.7" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "855 pg/mL", "standardDeviation": "68.4", "coefficientOfVariability": "= 8" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "56 pg/mL", "standardDeviation": "3.08", "coefficientOfVariability": "= 5.5" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "247 pg/mL", "standardDeviation": "23.46", "coefficientOfVariability": "= 9.5" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "947 pg/mL", "standardDeviation": "67.23", "coefficientOfVariability": "= 7.1" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213822-1x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB213822-CMP10" }, { "size":"1 x 100 µL", "name":"Biotinylated S100A12 Antibody", "number":"AB213822-CMP09" }, { "size":"1 x 1 Unit", "name":"Anti-Human S100A12 coated Microplate (12 x 8 wells)", "number":"AB213822-CMP08" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB213822-CMP04" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB213822-CMP07" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB213822-CMP02" }, { "size":"4 x 1 Unit", "name":"Adhesive Plate Seal", "number":"AB213822-CMP06" }, { "size":"2 x 1 Vial", "name":"Lyophilized recombinant human S100A12 standard", "number":"AB213822-CMP01" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB213822-CMP05" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB213822-CMP11" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB213822-CMP03" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213822-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB213822-CMP03", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB213822-CMP10", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB213822-CMP02", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Plate Seal", "number":"AB213822-CMP06", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated S100A12 Antibody", "number":"AB213822-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human S100A12 coated Microplate (12 x 8 wells)", "number":"AB213822-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB213822-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB213822-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized recombinant human S100A12 standard", "number":"AB213822-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB213822-CMP05", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The S100A12 protein also known as Calgranulin C or CGRP (Calcitonin Gene-Related Peptide) has a molecular mass of approximately 10.4 kDa. This protein shows expression primarily in neutrophils but is also present in other types of cells such as epithelial cells and certain cell lines. S100A12 belongs to the S100 protein family which is part of the EF-hand calcium-binding proteins known for playing roles in cell regulation. The protein binds calcium ions which influence its structural conformation.

Biological function summary

S100A12 is important in the inflammatory response and immune system regulation. It acts as a pro-inflammatory mediator attracting leukocytes to sites of inflammation. Furthermore it can bind to receptors on cell surfaces like RAGE (Receptor for Advanced Glycation End Products) which induces signaling cascades that amplify immune responses. S100A12 does not function alone but often forms homodimers or heterodimers with other S100 proteins enhancing its biological activities.

Pathways

S100A12 participates in pathways like the inflammatory response pathway and the RAGE signaling pathway. By interacting with RAGE and possibly other receptors it impacts cellular responses associated with stress injury and microbial infection. This interaction connects S100A12 to other proteins in the same pathways such as NF-kB an important transcription factor involved in inflammation and immune responses.

S100A12 has connections with conditions like rheumatoid arthritis and inflammatory bowel disease. Elevated levels of S100A12 often correlate with disease activity and severity suggesting its potential as a biomarker. In rheumatoid arthritis it contributes to the inflammatory environment while in inflammatory bowel disease it associates not only with inflammation but also with proteins like IL-6 and TNF-alpha which are significant cytokines in the pathogenesis of these diseases.

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Product protocols

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Target data

S100A12 is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its pro-inflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of pro-inflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. Acts as a monocyte and mast cell chemoattractant. Can stimulate mast cell degranulation and activation which generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation. Can inhibit the activity of matrix metalloproteinases; MMP2, MMP3 and MMP9 by chelating Zn(2+) from their active sites. Possesses filariacidal and filariastatic activity. Calcitermin possesses antifungal activity against C.albicans and is also active against E.coli and P.aeruginosa but not L.monocytogenes and S.aureus.

See full target information S100A12

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in medicine 9:795392 PubMed35242775

2022

The Expansion of Myeloid-Derived Suppressor Cells Correlates With the Severity of Pneumonia in Kidney Transplant Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Peng,Yulin Luo,Quan Zhuang,Junhui Li,Pengpeng Zhang,Min Yang,Yu Zhang,Gangcheng Kong,Ke Cheng,Yingzi Ming

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human S100A12 ELISA Kit

Sandwich ELISA - Human S100A12 ELISA Kit (AB213822)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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