Human S100A16 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human S100A16 in Human in Cell Lysate, Tissue samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Tissue
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1.25 - 20 ng/mL
- Assay time
- 4h
- Sensitivity
- = 0.22 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Calcium-binding protein. Binds one calcium ion per monomer (PubMed:17030513). Can promote differentiation of adipocytes (in vitro) (By similarity). Overexpression in preadipocytes increases their proliferation, enhances adipogenesis and reduces insulin-stimulated glucose uptake (By similarity).
Alternative names
S100F, AAG13, S100A16, Protein S100-A16, Aging-associated gene 13 protein, Protein S100-F, S100 calcium-binding protein A16
What's included?
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Human S100A16 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human S100A16 in Human in Cell Lysate, Tissue samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Tissue
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1.25 - 20 ng/mL
- Assay time
- 4h
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.22 ng/mL
- General recovery
- 96%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 20 | mean - | SD - | C.V. 4.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 20 | mean - | SD - | C.V. 10.3 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Human S100A16 ELISA Kit (ab284021) is designed for the detection of S100A16 in human cell lysate samples.
This assay employs a quantitative sandwich enzyme immunoassay technique that measures human S100A16 in approximately 4 hours. A polyclonal antibody specific for human S100A16 has been pre-coated onto a 96-well microplate with removable strips. S100A16 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human S100A16, which is recognized by a streptavidinperoxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
S100A16 also known as S100 calcium-binding protein A16 belongs to the S100 protein family. With a molecular mass of approximately 10.7 kDa S100A16 plays a role in the regulation of calcium homeostasis and cell differentiation. It is expressed in various tissues including the brain heart and adipose tissue. Like other S100 proteins S100A16 possesses two EF-hand motifs which facilitate its ability to bind to calcium ions influencing its structural conformation and function.
- Biological function summary
S100A16 is involved in cellular processes such as proliferation differentiation and migration. It participates in the modulation of cytoskeletal dynamics and cell cycle regulation. S100A16 often integrates into multiprotein complexes where it interacts with target proteins to exert its biological effects. Studies show that S100A16 may also contribute to epithelial-mesenchymal transition (EMT) an important event in development and cancer progression.
- Pathways
S100A16 functions in signaling pathways linked to calcium-dependent processes. In the MAPK signaling pathway it interacts with proteins like ERK1/2 to regulate cell proliferation and survival. Furthermore S100A16 is also associated with the TGF-beta pathway where its role in EMT becomes significant and involves interactions with TGF-beta-related factors. These pathways underline the importance of S100A16 in cellular homeostasis and developmental processes.
- Associated diseases and disorders
S100A16 is implicated in cancer and metabolic disorders. Its overexpression has been observed in several types of cancer including breast and colorectal cancer where it might contribute to tumor progression and metastasis. S100A16 also interacts with proteins like MMPs which are involved in tissue remodeling and invasion. Additionally S100A16 plays a part in metabolic diseases potentially through its role in adipocyte differentiation and lipid metabolism highlighting its multifaceted contribution to human health and disease.
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1 product image
Sandwich ELISA - Human S100A16 ELISA Kit (ab284021)
Example of Human S100A16 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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