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AB283547

Human S100A4 ELISA Kit

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(3 Publications)

Human S100A4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human S100A4 with a sensitivity of 0.41 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CAPL, MTS1, S100A4, Protein S100-A4, Calvasculin, Metastasin, Placental calcium-binding protein, Protein Mts1, S100 calcium-binding protein A4

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Sandwich ELISA - Human S100A4 ELISA Kit (AB283547)
  • sELISA

Supplier Data

Sandwich ELISA - Human S100A4 ELISA Kit (AB283547)

Interpolated concentrations of native S100A4 in human plasma - EDTA and cell culture supernatant samples.

The concentrations of S100A4 were measured in duplicates, interpolated from the S100A4 standard curves and corrected for sample dilution. Undiluted samples are as follows : plasma (EDTA) 40% and A172 supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean S100A4 concentration was determined to be 70.2 ng/mL in plasma (EDTA) and 3.95 ng/mL in A172 supernatant.

Sandwich ELISA - Human S100A4 ELISA Kit (AB283547)
  • sELISA

Supplier Data

Sandwich ELISA - Human S100A4 ELISA Kit (AB283547)

Example of human S100A4 standard curve in Sample Diluent 25BS.

The S100A4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.41 ng/mL

Range

0.94 - 60 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB197896

Anti-S100A4 antibody [EPR14639(2)]

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-S100A4 antibody [EPR14639(2)] (AB197896)

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Assay Kits

AB187394

Human TIMP1 ELISA Kit

Recombinant|SimpleStep

ELISA - Human TIMP1 ELISA Kit (AB187394)

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Primary Antibodies

AB18256

Anti-HMGB1 antibody

KO Validated

Western blot - Anti-HMGB1 antibody (AB18256)

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Primary Antibodies

AB5103

Anti-Histone H3 (citrulline R2 + R8 + R17) antibody

Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (AB5103)

  • 4
  • 28
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human S100A4 ELISA kit (ab283547) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human S100A4 protein in human serum, pasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human S100A4 with 0.41 ng/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

S100A4, encoded by the S100A4 gene, is a member of the S100 calcium binding family of proteins. S100A4 is known to be highly associated with components of the cytoskeleton to regulate the motility that can serve as a metastasis factor and invasiveness of cancer cells. When the gene is upregulated, it subsequently changes the cell's morphology, making it more susceptible to invasion from other proteins that contribute to metastasis.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.8" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "92 - 95 %", "average": "= 94" }, { "sample": "Serum", "range": "90 - 108 %", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "83 - 94 %", "average": "= 87" }, { "sample": "Citrate plasma", "range": "85 - 88 %", "average": "= 86" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

S100A4 also known as metastasin or MTS1 is a protein with a molecular weight of approximately 12 kDa. It functions by binding calcium which induces conformational changes aiding in its interaction with target proteins. S100A4 is part of the S100 family of proteins characterized by two EF-hand calcium-binding motifs. Expression of S100A4 is observed in various cell types including fibroblasts endothelial cells and several tumor cells where it contributes to processes like cell motility invasion and angiogenesis.
Biological function summary

The role of S100A4 extends beyond mere calcium binding; it associates with the cytoskeleton promoting cell structures related to movement and metastasis. It does not act alone but often partners with proteins like myosin IIA to regulate cytoskeletal dynamics. Additionally S100A4 modulates extracellular matrix components and interacts with signaling pathways that control cancer progression making it significant in both normal physiological contexts and pathological states.

Pathways

Functional activities of S100A4 integrate into important pathways involved in cellular movement and metastasis. S100A4 plays roles in the regulation of the Wnt/β-catenin pathway which is pivotal for cell-cell interaction and fate determination. It also interacts with matrix metalloproteinases like MMPs thereby managing processes of tissue invasion and remodeling essential in cancer metastasis. Other pathways influencing cell adhesion and signal transduction may indirectly involve S100A4 through related proteins.

Elevated S100A4 levels have implications in cancer particularly in enhancing metastasis in breast and colorectal cancers. The protein promotes tumor developments by inducing changes in the tumor microenvironment making it a target of interest in oncology. S100A4 also interacts with proteins like p53 and β-catenin which are critical in the pathology of tumors. Moreover S100A4 contributes to fibrotic diseases accentuating tissue remodeling activities by modulating fibroblast behavior and extracellular matrix deposition.
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Product protocols

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Target data

Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy (PubMed : 16707441, PubMed : 23752197, PubMed : 30713770). Increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain (NMMHC) IIA/MYH9 (PubMed : 16707441). Mechanistically, promotes filament depolymerization and increases the amount of soluble myosin-IIA, resulting in the formation of stable protrusions facilitating chemotaxis (By similarity). Also modulates the pro-apoptotic function of TP53 by binding to its C-terminal transactivation domain within the nucleus and reducing its protein levels (PubMed : 23752197). Within the extracellular space, stimulates cytokine production including granulocyte colony-stimulating factor and CCL24 from T-lymphocytes (By similarity). In addition, stimulates T-lymphocyte chemotaxis by acting as a chemoattractant complex with PGLYRP1 that promotes lymphocyte migration via CCR5 and CXCR3 receptors (PubMed : 26654597, PubMed : 30713770).
See full target information S100A4
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational medicine 14:e70058 PubMed39415352

2024

Insight into prostate cancer osteolytic metastasis by RelB coordination of IL-8 and S100A4.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbo Sun,Kenny Xu,Xiao Li,Peipei Qian,Fan Xu,Yanyan Zhang,Xiumei Wang,Zhi Xu,Jiaji Ding,Xinyu Xu,Xiaowei Wei,Qin Jiang,Yong Xu

Journal of translational medicine 21:721 PubMed37838657

2023

Cancer stem cell-derived CHI3L1 activates the MAF/CTLA4 signaling pathway to promote immune escape in triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shufeng Ji,Hao Yu,Dan Zhou,Xulong Fan,Yan Duan,Yijiang Tan,Min Lang,Guoli Shao

International journal of molecular sciences 24: PubMed37446230

2023

Inflammation of Dry Eye Syndrome: A Cellular Study of the Epithelial and Macrophagic Involvement of NFAT5 and RAGE.

Applications

Unspecified application

Species

Unspecified reactive species

Fanny Henrioux,Valentin Navel,Corinne Belville,Coline Charnay,Audrey Antoine,Frédéric Chiambaretta,Vincent Sapin,Loïc Blanchon
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