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Human S100B ELISA Kit - Extracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human S100B - Extracellular in Serum, Milk, Cerebral Spinal Fluid samples.
Alternative names=Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, S100B
- Detection method
Colorimetric
- Sample types
Serum, Milk, Cerebral Spinal Fluid
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
12.5 - 800 pg/mL
- Assay time
1h 30m
- Sensitivity
= 1.469 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Human S100B ELISA Kit - Extracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human S100B - Extracellular in Serum, Milk, Cerebral Spinal Fluid samples.
Alternative names=Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, S100B
Key facts
- Detection method
Colorimetric
- Sample types
Serum, Milk, Cerebral Spinal Fluid
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
12.5 - 800 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 1.469 pg/mL
- General recovery
85%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Milk | n 8 | mean - | SD - | C.V. 8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Milk | n 3 | mean - | SD - | C.V. 5.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 85 | Range 76 - 91 % |
Sample type Milk | Average % = 90 | Range 87 - 95 % |
Sample type Cerebral Spinal Fluid | Average % = 107 | Range 98 - 114 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human S100B SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of S100B protein in Serum, Milk, Cerebral Spinal Fluid. Quantitate Human S100B with 1.469 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Our SimpleStep ELISA® technology provides several benefits: Single-wash protocol reduces assay time to 90 minutes or less -High sensitivity, specificity and reproducibility from superior antibodies -Fully validated in biological samples -96-wells plate breakable into 12 x 8 wells strips A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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