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AB314385

Human S100P ELISA Kit

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(1 Publication)

Human S100P ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human S100P in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

S100E, S100P, Protein S100-P, Migration-inducing gene 9 protein, Protein S100-E, S100 calcium-binding protein P, MIG9

1 Images
Sandwich ELISA - Human S100P ELISA Kit (AB314385)
  • sELISA

Supplier Data

Sandwich ELISA - Human S100P ELISA Kit (AB314385)

Example of standard curve using ab314385. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 14 pg/mL

Range

14 - 3500 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human S100P ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human S100P in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human S100P coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "113 - 132 %", "average": "= 124" }, { "sample": "Plasma", "range": "97 - 120 %", "average": "= 112" }, { "sample": "Cell culture media", "range": "112 - 133 %", "average": "= 122.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314385-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Human S100P Antibody-coated ELISA Plate", "number":"AB314385-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314385-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human S100P Protein Standard", "number":"AB314385-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human S100P Detection Antibody", "number":"AB314385-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314385-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314385-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314385-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB314385-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

S100P also known as Protein S100-P is a member of the S100 family of proteins containing 94 amino acids with a mass of approximately 10 kDa. Its structure includes two EF-hand calcium-binding motifs which allow it to bind calcium ions effectively. You can find S100P in various tissues but it is highly expressed in the placenta prostate lungs and kidneys. These expression patterns suggest it has multiple roles in different cellular contexts.
Biological function summary

S100P plays a role in intracellular and extracellular calcium signaling. It influences cell survival proliferation and migration. It can function as a monomer but often interacts with other proteins forming complexes such as with RAGE (Receptor for Advanced Glycation End-products) which amplifies signaling cascades that control cellular responses. Its binding to calcium and interaction with RAGE modulate important cellular processes tied to cell cycle and cellular stress response.

Pathways

S100P is closely involved in the MAPK (Mitogen-Activated Protein Kinases) and RAGE signaling pathways. Its interaction with RAGE leads to activation of the MAPK pathway facilitating cellular responses such as growth and survival. S100P also coordinates with other S100 proteins such as S100A4 and S100A9 enhancing its involvement in regulating cellular functions through these pathways. These interactions highlight the importance of S100P in cellular communication and response mechanisms.

S100P is associated with various cancers like pancreatic and breast cancer. Its expression level often increases in malignancies where it promotes metastasis and poor prognosis. Through the RAGE pathway S100P influences tumor progression by altering cellular adhesion migration and immune response. In cancer biology it links with proteins such as E-cadherin and matrix metalloproteinases which further contribute to tumor invasiveness and metastasis making it a potential target for therapeutic intervention.
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Product protocols

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Target data

May function as calcium sensor and contribute to cellular calcium signaling. In a calcium-dependent manner, functions by interacting with other proteins, such as EZR and PPP5C, and indirectly plays a role in physiological processes like the formation of microvilli in epithelial cells. May stimulate cell proliferation in an autocrine manner via activation of the receptor for activated glycation end products (RAGE).
See full target information S100P
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of nanomedicine 19:10009-10021 PubMed39371477

2024

SERS Analysis Platform Based on Aptamer Recognition-Release Strategy for Efficient and Sensitive Diagnosis of Colorectal Precancerous Lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Fengsong Chen,Yanhua Huang,Yongxia Liu,Yanwen Zhuang,Xiaowei Cao,Xiaogang Qin
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