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AB309446

Human SAHH ELISA Kit

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Human SAHH ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human SAHH in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

SAHH, AHCY, Adenosylhomocysteinase, AdoHcyase, S-adenosyl-L-homocysteine hydrolase

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Sandwich ELISA - Human SAHH ELISA Kit (AB309446)
  • sELISA

Supplier Data

Sandwich ELISA - Human SAHH ELISA Kit (AB309446)

Example of standard curve using ab309446. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Results type

Quantitative

Sensitivity

= 0.61 ng/mL

Range

0.61 - 150 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human SAHH ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SAHH in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human SAHH coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "119 - 127 %", "average": "= 123.8" }, { "sample": "Plasma", "range": "77 - 119 %", "average": "= 107" }, { "sample": "Cell culture supernatant", "range": "97 - 117 %", "average": "= 107.4" }, { "sample": "Cell culture media", "range": "97 - 117 %", "average": "= 107.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309446-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309446-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human SAHH Protein Standard", "number":"AB309446-CMP17", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309446-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human SAHH Antibody-coated ELISA Plate", "number":"AB309446-CMP15", "productcode":"AB309446" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin", "number":"AB309446-CMP14", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309446-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB309446-CMP02", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB309446-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human SAHH Detection Antibody", "number":"AB309446-CMP16", "productcode":"" }, { "size":"1 x 1 Unit", "name":"ELISA Microplate Bag", "number":"AB309446-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

S-Adenosylhomocysteine hydrolase also known as SAHH plays a critical mechanical role in cellular metabolism. It catalyzes the reversible hydrolysis of S-adenosylhomocysteine (SAH) to homocysteine and adenosine. This enzyme approximately 48 kDa in mass is ubiquitously expressed in most tissues highlighting its universal importance in biological systems. SAHH maintains the cellular methylation balance by regulating the concentrations of SAH a byproduct of methylation reactions.
Biological function summary

S-Adenosylhomocysteine hydrolase acts as an important player in the methylation processes within the cell. By breaking down SAH it ensures proper methylation of DNA proteins and lipids through the recycling of homocysteine and adenosine. SAHH operates as a homotetramer forming a complex essential for its enzymatic function. The balance maintained by SAHH in cellular methylation allows for proper gene expression and signal transduction within various cellular processes.

Pathways

S-Adenosylhomocysteine hydrolase plays an essential role in the methionine cycle and one-carbon metabolism pathways. These pathways are interconnected with the folate cycle important for nucleotide synthesis and cellular methylation. SAHH works with enzymes like methionine synthase and adenosylmethionine linking it to regulation of homocysteine levels. These interconnections are fundamental for maintaining cellular function and homeostasis.

S-Adenosylhomocysteine hydrolase dysregulation can have significant implications. Hyperhomocysteinemia a condition characterized by high levels of homocysteine in the blood often results from SAHH dysfunction leading to cardiovascular diseases. Additionally aberrant SAHH activity connects to certain types of cancer as altered methylation patterns can disrupt normal cell proliferation. The interplay of SAHH with other proteins such as methylenetetrahydrofolate reductase (MTHFR) illustrates the complex network within which this enzyme operates affecting disease outcomes.
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Product protocols

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Target data

Catalyzes the hydrolysis of S-adenosyl-L-homocysteine to form adenosine and homocysteine (PubMed : 10933798). Binds copper ions (By similarity).
See full target information AHCY
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websiteProtocolBooklet
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Product promise

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