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AB314386

Human SCARA5 ELISA Kit

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Human SCARA5 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human SCARA5 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

UNQ2938/PRO28700, SCARA5, Scavenger receptor class A member 5, Scavenger receptor hlg

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Sandwich ELISA - Human SCARA5 ELISA Kit (AB314386)
  • sELISA

Supplier Data

Sandwich ELISA - Human SCARA5 ELISA Kit (AB314386)

Example of standard curve using ab314386. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.05 ng/mL

Range

0.05 - 10 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human SCARA5 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SCARA5 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human SCARA5 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "123 - 134 %", "average": "= 127.4" }, { "sample": "Plasma", "range": "88 - 134 %", "average": "= 110.2" }, { "sample": "Cell culture media", "range": "70 - 77 %", "average": "= 73.12" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314386-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314386-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human SCARA5 Protein Standard", "number":"AB314386-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human SCARA5 Antibody-coated ELISA Plate", "number":"AB314386-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314386-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314386-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin", "number":"AB314386-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human SCARA5 Detection Antibody", "number":"AB314386-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314386-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SCARA5 also known as scavenger receptor class A member 5 functions mechanically as a cell surface receptor involved in the endocytosis of iron-bound lipocalin-2. This protein has a mass of approximately 86 kDa. SCARA5 is expressed in various tissues including the placenta lung liver and prostate. It plays a role in binding and internalizing ligands leading to their transport into the cells where they are involved in further biological processes.
Biological function summary

SCARA5 plays a role in iron homeostasis as it modulates the cellular uptake of lipocalin-2 and its associated ferric-siderophore complexes. SCARA5 does not work alone; it operates as part of a complex involving ligands such as lipocalin-2 which can influence iron levels in cells and tissues. This interaction is necessary for maintaining proper iron balance and supporting cellular functions that rely on iron availability.

Pathways

SCARA5 is involved in the iron metabolism pathway and influences cellular iron uptake. This pathway interacts with other proteins like hepcidin and ferroportin which are essential for maintaining systemic iron balance. These interactions help to regulate iron absorption transport and storage within the body ensuring adequate iron is available for critical processes like red blood cell production and oxygen transport.

SCARA5 is associated with disorders like iron overload conditions and certain types of cancer. Abnormal SCARA5 expression or function may lead to disrupted iron homeostasis which can contribute to iron overload in tissues. Additionally SCARA5 expression has been linked to cancers such as hepatocellular carcinoma where it may interact with proteins like transferrin receptor to impact tumor growth and progression. Understanding its role in these conditions may provide insights into therapeutic targets and treatment strategies.
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Product protocols

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Target data

Ferritin receptor that mediates non-transferrin-dependent delivery of iron. Mediates cellular uptake of ferritin-bound iron by stimulating ferritin endocytosis from the cell surface with consequent iron delivery within the cell. Delivery of iron to cells by ferritin is required for the development of specific cell types, suggesting the existence of cell type-specific mechanisms of iron traffic in organogenesis, which alternatively utilize transferrin or non-transferrin iron delivery pathways. Ferritin mediates iron uptake in capsule cells of the developing kidney. Preferentially binds ferritin light chain (FTL) compared to heavy chain (FTH1).
See full target information SCARA5
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB118894

Anti-SCARA5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCARA5 antibody (AB118894)

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