Human SCF ELISA Kit

Product details

Human SCF ELISA Kit ab176109 is a rapid single-wash 90-min Sandwich ELISA to measure Human SCF in cell culture media, citrate plasma, heparin plasma, plasma, serum. This SimpleStep sensitivity is 0.51 pg/mL.

How the assay works

Human SCF SimpleStep ELISA^®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human SCF ELISA Kit ab176109 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

SCF is the ligand for the receptor-type protein-tyrosine kinase KIT. SCF is expressed in fibroblasts, liver cells, Sertoli cells, endothelial cells, neurons, macrophages, oocytes, Schwann cells and numerous carcinoma cell lines. SCF can be found as a cell membrane protein or as a soluble protein after secretion due to proteolytic processing in the extracellular domain. Both forms have growth factor activities; however the membrane form is also important for cell-cell adhesion and interaction.

Soluble SCF is a heavily N- and O-glycosylated protein that exists as a non-covalently linked homodimer or as a heterotetramer with its receptor. Binding to the receptor, results in KIT activation by autophosphorylation. This leads to multiple signaling cascades (via AKT, GRB2, RAS, MAPK amongst others) that are important in the regulation of cell survival, proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration, melanogenesis and neurite-inductive activity of dorsal root ganglia neurons.

Genetic defects in SCF are the cause of familial progressive hyperpigmentation (melanosis universalis hereditaria). SCF has also been involved in other conditions such as mastocytosis, mast cell leukemia, myeloid leukemia, aplastic anemia and gastrointestinal stromal tumor.