Human SCGB1A1 ELISA Kit (uteroglobin)

Product details

The Human SCGB1A1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (uteroglobin) (ab213823) is designed for the quantitative measurement of Human SCGB1A1 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk.

The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for SCGB1A1/uteroglobin has been pre-coated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: E22-N91) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SCGB1A1/uteroglobin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human SCGB1A1/uteroglobin amount of sample captured in plate.

SCGB1A1 (secretoglobin family 1A member 1) also known as Uteroglobin is a protein that in humans is encoded by the SCGB1A1 gene. SCGB1A1 is the founding member of the secretoglobin family of small, secreted, disulfide-bridged dimeric proteins found only in mammals. This protein is specifically expressed in Clara cells in the lungs. Numerous studies demonstrated that UG is a multifunctional protein with anti-inflammatory/ immunomodulatory properties. It inhibits soluble phospholipase A(2) activity and binds and perhaps sequesters hydrophobic ligands such as progesterone, retinols, polychlorinated biphenyls, phospholipids, and prostaglandins. In addition to its anti-inflammatory activities, UG manifests anti-chemotactic, anti-allergic, anti-tumorigenic, and embryonic growth stimulatory activities.