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AB314389

Human SDF1 beta ELISA Kit

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Human SDF1 beta ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human SDF1 beta in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

SDF1, SDF1A, SDF1B, CXCL12, Stromal cell-derived factor 1, SDF-1, hSDF-1, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, Pre-B cell growth-stimulating factor, IRH, hIRH, PBSF

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Sandwich ELISA - Human SDF1 beta ELISA Kit (AB314389)
  • sELISA

Supplier Data

Sandwich ELISA - Human SDF1 beta ELISA Kit (AB314389)

Example of standard curve using ab314389. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 35 pg/mL

Range

35 - 8000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human SDF1 beta ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SDF1 beta in serum, plasma (human SDF1 beta concentration is low in normal serum/plasma and may not be detectable in this assay), and cell culture supernatants. This assay employs an antibody specific for human SDF1 beta coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA-78, FGF-4, IL-1a, IL-1b, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF-7, GCSF, GDNF, GM-CSF, IFN-g, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1a, MIP-1 b, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF1 alpha, TARC, TGF-b, TIMP-1, TIMP-2, TNF-a, TNF-b, TPO, VEGF.

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Recovery

[ { "sample": "Serum", "range": "93 - 112 %", "average": "= 104.2" }, { "sample": "Plasma", "range": "72 - 101 %", "average": "= 85.03" }, { "sample": "Cell culture media", "range": "73 - 89 %", "average": "= 84.62" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SDF1 beta also known as CXCL12b or stromal cell-derived factor 1 beta is a small chemokine with a mass around 8 kDa. SDF1 beta plays a significant role as a signaling molecule involved in mediating various cellular responses. Expression of this chemokine occurs in a variety of tissues including spleen brain liver and skeletal muscle. Its mechanical function mainly involves binding to the CXCR4 receptor on target cells leading to the activation of signaling cascades that modulate cellular behavior.
Biological function summary

SDF1 beta participates in the regulation of immune cell trafficking and homing. As a prominent chemokine it directs leukocytes to sites where they perform their immunological duties such as inflamed or damaged tissue. Although it generally acts independently SDF1 beta can be part of a larger chemokine network collaborating with other chemokines and cytokines to orchestrate coordinated migration and positioning of immune cells.

Pathways

SDF1 beta is intricately linked with the chemokine signaling pathway and homing of hematopoietic stem cells. Activation of its pathway involves the prominent receptor CXCR4 which mediates signals that are important for the directional movement of these cells. SDF1 beta plays a central role in the hematopoiesis process where it interacts with molecules like CXCR7 which sometimes acts as a supportive player for its receptor influencing the hematopoietic stem cell niche regulation as well.

Involvement of SDF1 beta has been identified in cancer progression and inflammatory diseases like rheumatoid arthritis. In cancer it contributes to tumor growth and metastasis with CXCR4 being its main receptor partner. In rheumatoid arthritis overexpression or dysregulation of SDF1 beta influences the infiltration and retention of immune cells in the joints exacerbating inflammation. Understanding these interactions provides insights for potential therapeutic approaches targeting the SDF1 beta and CXCR4 axis in disease contexts.
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Product protocols

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Target data

Chemoattractant active on T-lymphocytes and monocytes but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV : ITGB3, ITGA4 : ITGB1 and ITGA5 : ITGB1 in a CXCR4-independent manner (PubMed : 29301984). Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Stimulates the proliferation of bone marrow-derived B-cell progenitors in the presence of IL7 as well as growth of stromal cell-dependent pre-B-cells (By similarity).
See full target information CXCL12
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websiteProtocolBooklet
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