AB100637

Human SDF1 ELISA Kit

Name: Human SDF1 ELISA Kit (ab100637) | Abcam
Brand: Abcam Limited
SKU: AB100637
Price: 780 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(18 Publications)

Human SDF1 ELISA Kit is a sandwich ELISA designed to quantify Human SDF1 with a sensitivity of 42 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 42 - 6000 pg/mL
- Cited in over 10 publications

View Alternative Names

SDF1, SDF1A, SDF1B, CXCL12, Stromal cell-derived factor 1, SDF-1, hSDF-1, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, Pre-B cell growth-stimulating factor, IRH, hIRH, PBSF

2 Images

Sandwich ELISA - Human SDF1 ELISA Kit (AB100637)

sELISA

Unknown

Sandwich ELISA - Human SDF1 ELISA Kit (AB100637)

Representative standard curve using ab100637

sELISA

Unknown

Sandwich ELISA - Human SDF1 ELISA Kit (AB100637)

Representative standard curve using ab100637

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 42 pg/mL

Range

42 - 6000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human SDF1 ELISA Kit ab100637 is a sandwich ELISA to measure Human SDF1 in plasma, cell culture supernatant with a sensitivity of 42 pg/ml.

How the assay works

This assay employs an antibody specific for Human SDF1 coated on a 96-well plate. Standards and samples are pipetted into the wells and SDF1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human SDF1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SDF1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human SDF1 ELISA Kit ab100637 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.

Optimization may be required with urine samples.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "86 - 104 %", "average": "= 94.63" }, { "sample": "Plasma", "range": "85 - 104 %", "average": "= 89.34" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100637-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100637-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100637-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100637-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Microplate (12 x 8 wells)", "number":"AB100637-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human SDF1 Standard (lyophilized)", "number":"AB100637-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100637-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human SDF1", "number":"AB100637-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100637-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100637-CMP07", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SDF1 beta and SDF1 alpha also known as CXCL12 are small chemokines playing important roles in several cellular processes. These signaling proteins have a molecular mass ranging from approximately 7 to 15 kDa depending on post-translational modifications. They are secreted proteins expressed in various tissues including bone marrow heart liver and brain. SDF1 beta and SDF1 alpha function by binding to the chemokine receptor CXCR4 mediating cellular responses through signal transduction mechanisms.

Biological function summary

SDF1 beta and SDF1 alpha regulate hematopoietic cell trafficking and homing. These proteins are significant for directing migration of immune cells and stem cells by forming part of a ligand-receptor complex with CXCR4. This interaction influences cell survival proliferation and inflammation. SDF1 also participates in organ development and promotes angiogenesis during tissue repair.

Pathways

SDF1 beta and SDF1 alpha are central to the CXCL12-CXCR4 axis important in signaling pathways like G-protein coupled receptor signaling and JAK-STAT signaling. These pathways contribute to immune response modulation and stem cell maintenance. Notably the interaction with CXCR4 is essential in the SDF-CXCR4 axis and effects are also mediated with other proteins like CXCR7 further impacting cellular signaling.

SDF1 beta and SDF1 alpha have associations with cancer metastasis and HIV infection. The SDF1-CXCR4 interaction represents an important point in metastatic spread since it aids tumor cell migration and invasion. This chemokine binding also serves as an essential co-receptor for HIV entry into cells implicating it in disease progression. Alterations in the SDF1-CXCR4 axis connect to oncogenic processes and viral infections alongside proteins such as CCR5 contributing to HIV pathway interactions.

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Product protocols

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Target data

Chemoattractant active on T-lymphocytes and monocytes but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV : ITGB3, ITGA4 : ITGB1 and ITGA5 : ITGB1 in a CXCR4-independent manner (PubMed : 29301984). Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Stimulates the proliferation of bone marrow-derived B-cell progenitors in the presence of IL7 as well as growth of stromal cell-dependent pre-B-cells (By similarity).

See full target information CXCL12

Additional targets

CXCL12

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Angiogenesis 28:26 PubMed40314870

2025

SDF-1α mRNA therapy in peripheral artery disease.

Applications

Unspecified application

Species

Unspecified reactive species

Tinghong Zhang,Binqiang Zhu,Shijie Deng,Jinling Qin,Jingyuan Zhang,Shu Meng

Frontiers in surgery 12:1507982 PubMed40166622

2025

Comparison of clinical efficacy between tibial cortex transverse transport and platelet-rich plasma treatment for severe diabetic foot ulcers.

Applications

Unspecified application

Species

Unspecified reactive species

Pu-Xiang Zhen,Hong-Jie Su,Si-Jie Yang,Xiang Chen,Zhan-Ming Lin,Sai-Nan Liu

Biomedicines 11: PubMed37189610

2023

Dimethyloxalylglycine Attenuates Steroid-Associated Endothelial Progenitor Cell Impairment and Osteonecrosis of the Femoral Head by Regulating the HIF-1α Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenkai Shao,Zilin Li,Bo Wang,Song Gong,Ping Wang,Beite Song,Zhixiang Chen,Yong Feng

Frontiers in immunology 13:1037812 PubMed36451827

2022

Increased peripheral inflammatory responses in myelin oligodendrocyte glycoprotein associated disease and aquaporin-4 antibody positive neuromyelitis optica spectrum disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Angelika Bauer,Dagmar Rudzki,Klaus Berek,Alessandro Dinoto,Christian Lechner,Eva-Maria Wendel,Harald Hegen,Florian Deisenhammer,Thomas Berger,Romana Höftberger,Kevin Rostasy,Sara Mariotto,Markus Reindl

EMBO reports 23:e55871 PubMed36200589

2022

Endothelial-immune crosstalk contributes to vasculopathy in nonalcoholic fatty liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Yi Ng,Khang Leng Lee,Mark Dhinesh Muthiah,Kan Xing Wu,Florence Wen Jing Chioh,Konstanze Tan,Gwyneth Shook Ting Soon,Asim Shabbir,Wai Mun Loo,Zun Siong Low,Qingfeng Chen,Walter Wahli,Nguan Soon Tan,Huck Hui Ng,Yock Young Dan,Christine Cheung

Animal cells and systems 26:148-157 PubMed36046033

2022

CXCL12 secreted by pancreatic stellate cells accelerates gemcitabine resistance of pancreatic cancer by enhancing glycolytic reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyu Lu,Yilei Wu,Rui Cao,Xiaojiong Yu,Jun Gong

EMBO reports 23:e54271 PubMed35403791

2022

Endothelial-immune crosstalk contributes to vasculopathy in nonalcoholic fatty liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Yi Ng,Khang Leng Lee,Mark Dhinesh Muthiah,Kan Xing Wu,Florence Wen Jing Chioh,Konstanze Tan,Gwyneth Shook Ting Soon,Asim Shabbir,Wai Mun Loo,Zun Siong Low,Qingfeng Chen,Nguan Soon Tan,Huck Hui Ng,Yock Young Dan,Christine Cheung

Molecular medicine reports 22:4163-4172 PubMed32901877

2020

SDF1/CXCR4 axis facilitates the angiogenesis via activating the PI3K/AKT pathway in degenerated discs.

Applications

Unspecified application

Species

Unspecified reactive species

Hanxiang Zhang,Peng Wang,Xiang Zhang,Wenrui Zhao,Honglei Ren,Zhenming Hu

Medicine 99:e20944 PubMed32629699

2020

Inhibition of ITGB1 enhance the anti-tumor effect of cetuximab in colorectal cancer cell.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Yang,Shuai Wang,Weihua Yu,Yixiong Zheng,Yulian Wu

Stem cells international 2020:2803747 PubMed32377202

2020

Enhanced Effect of IL-1-Activated Adipose-Derived MSCs (ADMSCs) on Repair of Intestinal Ischemia-Reperfusion Injury via COX-2-PGE Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Liu Liu,Yi Ren He,Shao Jun Liu,Lei Hu,Li Chuang Liang,Dong Liang Liu,Lin Liu,Zhi Qiang Zhu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human SDF1 ELISA Kit

Sandwich ELISA - Human SDF1 ELISA Kit (AB100637)

Sandwich ELISA - Human SDF1 ELISA Kit (AB100637)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (18)

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Product promise