JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB314390

Human SECTM1 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human SECTM1 ELISA Kit is a Sandwich ELISA for the measurement of Human SECTM1 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

K12, SECTM1, Secreted and transmembrane protein 1, Protein K-12

1 Images
Sandwich ELISA - Human SECTM1 ELISA Kit (AB314390)
  • sELISA

Supplier Data

Sandwich ELISA - Human SECTM1 ELISA Kit (AB314390)

Example of standard curve using ab314390. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.85 ng/mL

Range

1.85 - 500 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Cell Lines & Lysates

AB259104

Human SECTM1 knockout A549 cell lysate

Sanger Sequencing - Human SECTM1 knockout A549 cell lysate (AB259104)

  • 0
  • 0
Cell Lines & Lysates

AB267021

Human SECTM1 knockout A549 cell line

Sanger Sequencing - Human SECTM1 knockout A549 cell line (AB267021)

  • 0
  • 0
Assay Kits

AB280327

Daratumumab (Human) ELISA Kit

Sandwich ELISA - Daratumumab (Human) ELISA Kit (AB280327)

  • 0
  • 0
Assay Kits

AB213885

Mouse Relaxin 1 ELISA Kit

Sandwich ELISA - Mouse Relaxin 1 ELISA Kit (AB213885)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human SECTM1 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SECTM1 in serum, plasma (collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended in this assay), and cell culture supernatants. This assay employs an antibody specific for human SECTM1 coated on a 96-well plate.
style
left-column

Recovery

[ { "sample": "Serum", "range": "71 - 120 %", "average": "= 87.6" }, { "sample": "Plasma", "range": "91 - 140 %", "average": "= 118.1" }, { "sample": "Cell culture media", "range": "81 - 100 %", "average": "= 90.05" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314390-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Human SECTM1 Antibody-coated ELISA Plate", "number":"AB314390-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314390-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human SECTM1 Protein Standard", "number":"AB314390-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human SECTM1 Detection Antibody", "number":"AB314390-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314390-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314390-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314390-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin", "number":"AB314390-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SECTM1 also known as Secreted and Transmembrane 1 and sometimes as K12 is a protein with a molecular mass of approximately 28 kDa. It expresses both membrane-bound and soluble forms found mainly in epithelial cells and in some types of immune cells such as monocytes. SECTM1 resides in the Golgi apparatus and can be secreted outside the cell indicating its role in cell signaling and communication.
Biological function summary

The protein plays a role in modulating immune responses. SECTM1 interacts with CD7 a cell surface glycoprotein found on T cells and natural killer cells influencing their activation and proliferation. This interaction is important for immune system regulation and SECTM1 can function together with other immune-related proteins but usually operates independently rather than as part of a larger protein complex.

Pathways

SECTM1 is involved in immunological pathways particularly those governing T-cell activation and the broader inflammatory response. Within these pathways SECTM1 connects with proteins like CD7 and is associated with the PI3K/Akt signaling cascade. This linkage influences cell survival and growth bridging innate and adaptive immunity.

SECTM1 has associations with several inflammatory and autoimmune conditions as well as cancer. Abnormal expression levels of SECTM1 have implications in disorders such as rheumatoid arthritis where its interaction with CD7 appears to play a role in disease progression. Similarly in certain cancers altered SECTM1 expression leads to changes in immune cell behavior affecting tumor growth and metastasis.
style
left-column

Product protocols

style
left-column

Target data

May be involved in thymocyte signaling.
See full target information SECTM1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com