Human SEMA4C ELISA Kit is a single-wash 90-min Simplestep used to quantify Human SEMA4C with a sensitivity of 23.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 93.75 - 6000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 23.5 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cell surface receptor for PLXNB2 that plays an important role in cell-cell signaling. PLXNB2 binding promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal brain development, axon guidance and cell migration (By similarity). Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade.
Alternative names
KIAA1739, SEMAI, UNQ5855/PRO34487, SEMA4C, Semaphorin-4C
What's included?
Recommended products
Human SEMA4C ELISA Kit is a single-wash 90-min Simplestep used to quantify Human SEMA4C with a sensitivity of 23.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 93.75 - 6000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 23.5 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Plasma | n 8 | mean - | SD - | C.V. 3.7 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Plasma | n 3 | mean - | SD - | C.V. 9.1 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 95 | Range 93 - 97 % |
Sample type Serum | Average % = 87 | Range 84 - 94 % |
Sample type EDTA Plasma | Average % = 85 | Range 80 - 92 % |
Sample type Heparin Plasma | Average % = 94 | Range 92 - 99 % |
Sample type Citrate plasma | Average % = 81 | Range 80 - 82 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human SEMA4C ELISA kit (ab284623) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human SEMA4C protein in human serum, plasma, cell culture supernatant and cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human SEMA4C with 23.5 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SEMA4C also known as SEMAI is a member of the semaphorin family which are proteins involved in cellular signaling. SEMA4C has a molecular mass of approximately 89 kDa and features in numerous cellular contexts. It is expressed in various tissues including the neural tissue muscle tissue and certain epithelial tissues. This expression suggests a diverse role in different cellular environments contributing to various physiological processes.
- Biological function summary
SEMA4C plays a significant role in mediating cell-cell communication particularly in processes like axon guidance and cell migration. It acts as a ligand in binding to plexin receptors forming part of signaling complexes that regulate cellular dynamics. This protein can influence cytoskeletal reorganization which is also essential for connections between neurons and the development of the nervous system. SEMA4C is important for both developmental processes and responses in adults where its regulation impacts cellular behavior and function.
- Pathways
SEMA4C modulates signaling cascades such as the MAPK pathway and the PI3K/AKT pathway. These pathways are critical in controlling growth and survival of cells. SEMA4C interacts with other proteins such as Plexin-B1 and Plexin-B2 signaling through these pathways and impacting neuronal development and immune response. It acts as a mediator between the extracellular environment and intracellular signaling mechanisms playing an important role in cellular responses to external stimuli.
- Associated diseases and disorders
SEMA4C is associated with cancer and neurodegenerative diseases. In cancer overexpression of SEMA4C can promote tumor progression and metastasis often linked with other proteins like Plexin-B1 in the tumor microenvironment. In the context of neurodegenerative diseases alterations or dysregulation of SEMA4C signaling can affect neuronal survival and connectivity and it might interact with proteins like tau contributing to disease progression. Understanding the function of SEMA4C in these conditions may provide avenues for therapeutic interventions.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Sandwich ELISA - Human SEMA4C ELISA Kit (ab284623)
Example of human SEMA4C standard curve in Sample Diluent NS.
Example of human SEMA4C standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human SEMA4C ELISA Kit (ab284623)
Example of human SEMA4C standard curve in 1X Cell Extraction Buffer PTR.
Example of human SEMA4C standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human SEMA4C ELISA Kit (ab284623)
Interpolated concentrations of human SEMA4C in serum, plasma-EDTA, plasma-Heparin, and MCF-7 cell culture supernatant.
Interpolated concentration of native SEMA4C was measured in duplicate at different sample concentrations. Undiluted samples are 100% serum, plasma-EDTA, plasma-Heparin, and MCF-7 cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human SEMA4C ELISA Kit (ab284623)
Interpolated concentrations of human SEMA4C in MCF-7 cell extract.
Interpolated concentration of native SEMA4C was measured in duplicate at different sample concentrations. Undiluted samples are 500 μg/mL MCF-7 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Human SEMA4C ELISA Kit (ab284623)
Interpolated concentrations of SEMA4C in normal human serum donors.
Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean SEMA4C concentration was determined to be 301.3 pg/mL with a range of 126.8 – 570.3 pg/mL.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com