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AB314391

Human SEMA4G ELISA Kit

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Human SEMA4G ELISA Kit is a Sandwich ELISA for the measurement of Human SEMA4G in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

KIAA1619, SEMA4G, Semaphorin-4G

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Sandwich ELISA - Human SEMA4G ELISA Kit (AB314391)
  • sELISA

Supplier Data

Sandwich ELISA - Human SEMA4G ELISA Kit (AB314391)

Example of standard curve using ab314391. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.085 ng/mL

Range

0.085 - 20 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Proteins & Peptides

AB163637

Recombinant Human SEMA4G protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human SEMA4G protein (GST tag N-Terminus) (AB163637)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human SEMA4G ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SEMA4G in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human SEMA4G coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Activin RIA, ASAHL, B4GalT1, BAI1, Brorin, C1qTNF4, CA14, CA4, CA6, CA8, Cadherin-6, Caspr2, CD27 Ligand, CD300a, CD300e, CD300f, CD4, CD5, CD69, CK18, CK19, CPB1, CRISP-2, DDR1, FUT8, MIA, NTAL, NTB-A, OMgp, PEAR1, Podoplanin, PTH1R, Reg4, ROR1, Serpin A5, Serpin B6, Siglec-1, Sirtuin 2, Sirtuin 5.

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Recovery

[ { "sample": "Serum", "range": "107 - 143 %", "average": "= 122.6" }, { "sample": "Plasma", "range": "97 - 128 %", "average": "= 114.9" }, { "sample": "Cell culture media", "range": "91 - 143 %", "average": "= 123.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314391-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314391-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314391-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314391-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human SEMA4G Antibody-coated ELISA Plate", "number":"AB314391-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human SEMA4G Protein Standard", "number":"AB314391-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB314391-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314391-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human SEMA4G Detection Antibody", "number":"AB314391-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEMA4G also known as Semaphorin-4G functions mechanically as a signaling protein belonging to the semaphorin family. It plays a role in axon guidance and cellular interactions. The protein has a mass of approximately 84 kDa. SEMA4G is highly expressed in the nervous system with notable presence in regions like cerebellum and hippocampus suggesting roles in neural development and function.
Biological function summary

SEMA4G influences neuronal connectivity and cell signaling processes. It does not operate as part of a complex but interacts with receptors like plexins to influence cellular responses. SEMA4G helps in axonal pathfinding and synaptic organization playing key roles in the formation of neural circuits. These interactions suggest that SEMA4G assists in maintaining proper communication and organization within the nervous system.

Pathways

Researchers place SEMA4G within axon guidance and semaphorin-plexin signaling pathways. Within these SEMA4G interacts with protein partners such as Plexin-B1 which helps mediate downstream signaling events essential for neurite outgrowth and retraction. These signaling events ensure proper neural circuit formation and are important for healthy neural function.

Altered SEMA4G expression or function links to neurological disorders including autism spectrum disorders. Aberrant semaphorin signaling can disrupt neuronal connectivity contributing to developmental issues. In neurodegenerative conditions like Alzheimer's disease altered SEMA4G levels associate with disrupted synaptic organization. Connections to proteins like Plexin-B1 and their altered signaling can contribute to these disease states indicating the importance of precise SEMA4G function in maintaining neural health.
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Product protocols

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Target data

Cell surface receptor for PLXNB2. May play a role in axon guidance (By similarity).
See full target information SEMA4G
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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