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AB314393

Human SEMA6B ELISA Kit

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Human SEMA6B ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human SEMA6B in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

SEMAN, SEMAZ, UNQ1907/PRO4353, SEMA6B, Semaphorin-6B, Semaphorin-Z, Sema Z

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Sandwich ELISA - Human SEMA6B ELISA Kit (AB314393)
  • sELISA

Supplier Data

Sandwich ELISA - Human SEMA6B ELISA Kit (AB314393)

Example of standard curve using ab314393. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.6 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human SEMA6B ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SEMA6B in serum (human SEMA6B concentration is low in normal serum/plasma, and may not be detectable in this assay concentration) , plasma and cell culture supernatants. This assay employs an antibody specific for human SEMA6B coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human ACE, Activin RIB (ALK-4), ADAM23, Artemin, Cardiotrophin-1 (CT-1), Cathepsin V, FABP1, FGF20, GDF-8, HAI-1, IL-27 R alpha (WSX-1), Insulin R, Kallikrein 7, LIF R alpha, Lipocalin-1, LTbR (Lymphotoxin beta R), Mesothelin, MFRP, Neuropilin-2, Neurturin, Nidogen-2, Olfactomedin-2 (Noelin-2), p53, PDECGF (Thymidine Phosphorylase), PDGF-CC, Progranulin, Ret, ROBO4, Serpin F1 (PEDF), SREC-I (SCARF1), SREC-II (SCARF2), TLR1, TLR3, TPP1 (Tripeptidyl-peptidase I), TREM-2, TrkC, TROY (TNFRSF19), Uromodulin, XIAP.

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Recovery

[ { "sample": "Serum", "range": "68 - 81 %", "average": "= 72.21" }, { "sample": "Plasma", "range": "68 - 80 %", "average": "= 71.17" }, { "sample": "Cell culture media", "range": "81 - 91 %", "average": "= 84.89" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314393-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB314393-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314393-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human SEMA6B Protein Standard", "number":"AB314393-CMP11", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB314393-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human SEMA6B Detection Antibody", "number":"AB314393-CMP10", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A (Item D)", "number":"AB314393-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314393-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314393-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human SEMA6B Antibody-coated ELISA Plate", "number":"AB314393-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEMA6B also known as Semaphorin-6B is a member of the semaphorin family which is encoded by the SEMA6B gene. It has an approximate molecular mass of 110 kDa. Mechanically SEMA6B functions as a signaling molecule involved in axon guidance. It transmits guidance cues that direct neuronal growth cones during neural development. Expression of SEMA6B occurs in various tissues with significant levels in the nervous system highlighting its involvement in neural patterning.
Biological function summary

Semaphorin-6B facilitates cellular signaling that impacts cell proliferation differentiation and migration. It operates as a part of larger signaling cascades rather than a solitary entity. This protein interacts closely with neuropilin and plexin receptor families to modulate cellular communication and connectivity. These interactions suggest its role is not isolated but integrated into complex networks that influence the structural development of the organism.

Pathways

The activity of SEMA6B intersects with neural development pathways such as the axon guidance signaling pathway. It shares interactions with proteins like Plexin-A2 which is important for the mechanism of transmitting semaphorin signals. Additionally it has implications in the semaphorin-plexin signaling pathway where it contributes to signal transduction processes important for brain development and synaptic formation.

Semaphorin-6B has been linked to processes implicated in neurodevelopmental disorders such as autism spectrum disorder. Its involvement occurs alongside proteins like Neuropilin-1 which also plays a significant role in axon guidance. Alterations in SEMA6B expression and function are observed in certain cancer types where it influences tumor growth and metastasis. This demonstrates its broader impact on pathological conditions by affecting cellular communication and movement.
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Product protocols

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Target data

Functions as a cell surface repellent for mossy fibers of developing neurons in the hippocampus where it plays a role in axon guidance. May function through the PLXNA4 receptor expressed by mossy cell axons.. (Microbial infection) Acts as a receptor for P.sordellii toxin TcsL in the in the vascular endothelium.
See full target information SEMA6B
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websiteProtocolBooklet
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Product promise

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