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AB314394

Human SEMA6D ELISA Kit

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Human SEMA6D ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human SEMA6D in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

KIAA1479, SEMA6D, Semaphorin-6D

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Sandwich ELISA - Human SEMA6D ELISA Kit (AB314394)
  • sELISA

Supplier Data

Sandwich ELISA - Human SEMA6D ELISA Kit (AB314394)

Example of standard curve using a314394. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.64 ng/mL

Range

1.64 - 400 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human SEMA6D ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SEMA6D in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human SEMA6D coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human ALK-1, B7-H2, BLAME, BMP-8, CD28, Common beta Chain (CD131), Contactin-1, Desmoglein-1, Desmoglein-3, EDAR, EphA1, EphB6, Ephrin-B3, Epiregulin, FGF-12, FGF-17, FOLR2, Galectin-8, Glypican 1, Glypican 5, GHR, IFNgamma R1, IL-22 R alpha 1, IL-22BP, IL-23 R, IL-31 RA, IL-7R alpha, Integrin alpha 5, MDM2, Nectin-1, NKp30, Nogo Receptor, Notch-3, OSM R beta, Prolactin R, RELT, Ryk, Semaphorin 7A, Siglec-11.

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Recovery

[ { "sample": "Serum", "range": "72 - 87 %", "average": "= 78.29" }, { "sample": "Plasma", "range": "68 - 93 %", "average": "= 78.13" }, { "sample": "Cell culture media", "range": "68 - 86 %", "average": "= 79.08" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314394-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Lyophilized Human SEMA6D Protein Standard", "number":"AB314394-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314394-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314394-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB314394-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human SEMA6D Detection Antibody", "number":"AB314394-CMP10", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB314394-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin", "number":"AB314394-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314394-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human SEMA6D Antibody-coated ELISA Plate", "number":"AB314394-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein SEMA6D also known as Semaphorin-6D is a member of the semaphorin family characterized by its role in cell signaling. SEMA6D has a molecular mass of approximately 119 kDa. It is expressed in various tissues including the heart nervous system and certain immune cells. Mechanically SEMA6D functions through its interactions with plexin receptors particularly Plexin-A1 facilitating signal transduction that regulates cellular movement and organization.
Biological function summary

Semaphorin-6D contributes to multiple processes like axon guidance cardiac morphogenesis and immune function. It does not exist as part of a larger complex but acts through receptor interactions that lead to activation of downstream signaling pathways. Semaphorin-6D plays an influential role in guiding neuronal pathfinding during development ensuring proper formation of neural networks and also affects the organization and function of cardiac tissues.

Pathways

Semaphorin-6D modulates the activity in signaling systems like the RhoA/ROCK pathway and the MAPK pathway. These pathways are important for cytoskeletal dynamics and cell migration. It interacts with Plexin-A1 which assists in signal transmission leading to alterations in cell shape and motility. The involvement of SEMA6D in these pathways indicates its significance in cellular rearrangements and tissue architecture.

Semaphorin-6D has associations with cardiac malformations and certain neurological disorders. Abnormal SEMA6D signaling can contribute to congenital heart defects where improper cardiac development is linked to its interaction with Plexin-A1 and downstream effectors. Additionally its dysregulation has been observed in neurodegenerative diseases emphasizing its involvement in maintaining neural structure and function. These connections highlight Semaphorin-6D's importance in both developmental and pathological contexts.
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Product protocols

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Target data

Shows growth cone collapsing activity on dorsal root ganglion (DRG) neurons in vitro. May be a stop signal for the DRG neurons in their target areas, and possibly also for other neurons. May also be involved in the maintenance and remodeling of neuronal connections. Ligand of TREM2 with PLXNA1 as coreceptor in dendritic cells, plays a role in the generation of immune responses and skeletal homeostasis (By similarity).
See full target information SEMA6D
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB198745

Anti-SEMA6D antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEMA6D antibody - N-terminal (AB198745)

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Proteins & Peptides

AB164191

Recombinant Human SEMA6D protein

SDS-PAGE - Recombinant Human SEMA6D protein (AB164191)

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