JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB282868

Human Semaphorid 4D/CD10 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human Semaphorid 4D/CD10 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Semaphorid 4D/CD10 with a sensitivity of 22.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD100, C9orf164, SEMAJ, SEMA4D, Semaphorin-4D, A8, BB18, GR3

3 Images
Sandwich ELISA - Human Semaphorid 4D/CD10 ELISA Kit (AB282868)
  • sELISA

Supplier Data

Sandwich ELISA - Human Semaphorid 4D/CD10 ELISA Kit (AB282868)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Semaphorin 4D/CD10 concentration was determined to be 12.38 ng/mL with a range of 3.35 – 43.52 ng/mL.

Sandwich ELISA - Human Semaphorid 4D/CD10 ELISA Kit (AB282868)
  • sELISA

Supplier Data

Sandwich ELISA - Human Semaphorid 4D/CD10 ELISA Kit (AB282868)

Interpolated concentrations of native Semaphorin 4D/CD10 in human serum and plasma samples.

The concentrations of Semaphorin 4D/CD10 were measured in duplicates, interpolated from the Semaphorin 4D/CD10 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 12.5%, plasma (heparin) 25%, and plasma (EDTA) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Semaphorin 4D/CD10 concentration was determined to be 10.88 ng/mL in serum, 14.75 ng/mL in plasma (citrate), 10.21 ng/mL in plasma (heparin), and 11.02 ng/mL in plasma (EDTA).

Sandwich ELISA - Human Semaphorid 4D/CD10 ELISA Kit (AB282868)
  • sELISA

Supplier Data

Sandwich ELISA - Human Semaphorid 4D/CD10 ELISA Kit (AB282868)

Example of human Semaphorin 4D/CD10 standard curve in Sample Diluent NS.

The Semaphorin 4D/CD10 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 22.9 pg/mL

Range

78.125 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human Semaphorid 4d/CD10 ELISA kit (ab282868) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Semaphorid 4d/CD10 protein in human serum, plasma, and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Semaphorid 4d/CD10 with 22.9 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Semaphorin 4D/CD10 is an R-Ras GTPase-activating protein receptor for PLXNB1 and PLXNB2. Semaphorin 4D is involved in neural development, migration of cerebellar granule cells, and in the immune system. SEMA4D-PLXNB1 interactions are involved in tumor progression through tumor angiogenesis, tumor invasiveness and macrophage regulation.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "95 - 110 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "93 - 113 %", "average": "= 101" }, { "sample": "Cell culture media", "range": "91 - 103 %", "average": "= 97" }, { "sample": "Heparin Plasma", "range": "92 - 100 %", "average": "= 95" }, { "sample": "Citrate plasma", "range": "93 - 103 %", "average": "= 98" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab282868-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB282868-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB282868-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB282868-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB282868-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB282868-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Semaphorin 4D/CD10 Capture Antibody", "number":"AB282868-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Semaphorin 4D/CD10 Lyophilized Recombinant Protein", "number":"AB282868-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB282868-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB282868-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Semaphorin 4D/CD10 Detector Antibody", "number":"AB282868-CMP04", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Semaphorin 4D also known as CD100 is a transmembrane protein that plays important roles in cellular signaling. It belongs to the semaphorin protein family known for their role in axonal guidance. CD100 has a molecular mass of approximately 150 kDa. This protein expresses mainly in the immune system including T cells and B cells but can also be found in certain neurons and endothelial cells. It can exist in both a membrane-bound form and a soluble form which arise from proteolytic cleavage.
Biological function summary

CD100 influences various cellular functions by interacting with multiple receptors such as Plexin-B1 and CD72 as part of a larger signaling complex. It contributes to immune response regulation by promoting T-cell activation and B-cell aggregation which are essential for adaptive immunity. In the nervous system it guides neural development including axon growth and synaptic plasticity. CD100's interaction with Plexin-B1 plays a significant role in these developmental processes often affecting cell morphology and movement.

Pathways

Semaphorin 4D is involved in both immune and neural signaling pathways. In the immune system it influences the PI3K-Akt signaling pathway which impacts cell survival and immune cell activation. It associates with pathways involving Plexin-B1 and RhoA to regulate cytoskeletal dynamics affecting cell motility and growth. Additionally CD100's role in neural pathways intersects with proteins like neuropilin-1 influencing semaphorin-mediated signaling essential for neural connectivity.

Semaphorin 4D has links to cancer and autoimmune diseases. High expression levels of CD100 are observed in several tumor types where it might promote tumor growth and metastasis through its signaling pathways. In autoimmune conditions like multiple sclerosis CD100 affects immune cell interactions and can exacerbate inflammatory responses. Its involvement with proteins such as CD72 and Plexin-B1 in these conditions highlights its contribution to pathogenesis and potential as a therapeutic target.
style
left-column

Product protocols

style
left-column

Target data

Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling (PubMed : 20877282). Regulates GABAergic synapse development (By similarity). Promotes the development of inhibitory synapses in a PLXNB1-dependent manner (By similarity). Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA (PubMed : 19788569). Promotes the migration of cerebellar granule cells (PubMed : 16055703). Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro) (PubMed : 8876214). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway (PubMed : 16055703).
See full target information SEMA4D
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Primary Antibodies

AB134128

Anti-Semaphorin 4D/CD100 antibody [EP3569]

KO Validated|RabMAb|Recombinant

Western blot - Anti-Semaphorin 4D/CD100 antibody [EP3569] (AB134128)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com