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AB272193

Human Semaphorin 7A ELISA Kit

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Human Semaphorin 7A ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Semaphorin 7A with a sensitivity of 24.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD108, SEMAL, SEMA7A, Semaphorin-7A, CDw108, JMH blood group antigen, John-Milton-Hargen human blood group Ag, Semaphorin-K1, Semaphorin-L, Sema K1, Sema L

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Sandwich ELISA - Human Semaphorin 7A ELISA Kit (AB272193)
  • sELISA

Supplier Data

Sandwich ELISA - Human Semaphorin 7A ELISA Kit (AB272193)

Interpolated concentrations of native Semaphorin 7A in human serum and plasma samples.

The concentrations of Semaphorin 7A were measured in duplicate, interpolated from the Semaphorin 7A standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 10%, plasma (citrate) 10%, plasma (EDTA) 10%, and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Semaphorin 7A concentration was determined to be 14,101 pg/mL in serum, 11,909 pg/mL in plasma (citrate) and 14,381 pg/mL in plasma (EDTA), and 10,604 pg/ml in plasma (heparin).

Sandwich ELISA - Human Semaphorin 7A ELISA Kit (AB272193)
  • sELISA

Supplier Data

Sandwich ELISA - Human Semaphorin 7A ELISA Kit (AB272193)

Example of human Semaphorin 7A standard curve in Sample Diluent NS.

The Semaphorin 7A standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 24.7 pg/mL

Range

70.31 - 4500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Semaphorin 7A ELISA kit (ab272193) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Semaphorin 7A protein in serum and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Semaphorin 7A with 24.7 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Semaphorins constitute a large class of soluble and membrane bound proteins that are known to be involved in critical immune responses and serve neuronal functions. Semaphorin 7A, also known as CD108, is a glycosylphosphatidylinositol-anchored semaphorin that promotes axon outgrowth through beta1-integrin receptors and contributes to the formation of the lateral olfactory tract. Promotes attachment, spreading and dendrite outgrowth in melanocytes and plays an important role in modulating inflammation and T-cell mediated immune response. Mouse, rat. cow and monkey share 89, 89%, 86% and 99% sequence homology, respectively.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.62" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.52" } ]
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Recovery

[ { "sample": "Serum", "range": "104 - 112 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "103 - 105 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "101 - 105 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "116 - 119 %", "average": "= 117" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Semaphorin 7a also known as SEMA7A or CD108 is a glycoprotein that has a molecular mass of approximately 80 kDa. This target plays a role in axonal guidance and immune response regulation. It is a member of the semaphorin family which generally acts as signaling molecules. Semaphorin 7a is expressed in various tissues including the immune system nervous system and some epithelial cells. Its expression is especially significant in T-cells and is involved in modulating cell adhesion and migration.
Biological function summary

Semaphorin 7a is implicated in diverse processes such as immune system modulation and nervous system development. It functions as part of a complex involving integrins which mediate cell-to-cell communication and interaction with the extracellular matrix. This protein can bridge the immune and neural signaling pathways indicating its multifunctional nature within organisms. Its interaction with β1 integrins enhances T-cell activation and cytokine production which is important for immune response.

Pathways

Semaphorin 7a integrates into the MAPK and PI3K signaling pathways. These pathways are essential for cell survival migration and apoptosis. In the MAPK pathway Semaphorin 7a impacts downstream signaling through its interaction with ERK proteins. Its role in the PI3K pathway connects it to AKT proteins influencing cell growth and metabolic processes. These connections show its versatility and critical role in signal transduction linked to cellular behaviors.

Semaphorin 7a has been associated with multiple sclerosis and melanoma. In multiple sclerosis altered expression of this protein can affect immune response and neural repair processes. Its connection to melanoma is linked with its role in regulating cell motility and adhesion impacting cancer progression and metastasis. Semaphorin 7a's interactions with proteins like Melanocortin 1 Receptor (MC1R) in melanoma provide insights into therapeutic targets for disease interventions.
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Product protocols

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Target data

Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of pro-inflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes.
See full target information SEMA7A
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websiteProtocolBooklet
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