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AB240682

Human SEMG2 ELISA Kit

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Human SEMG2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human SEMG2 with a sensitivity of 8.4 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Semenogelin-2, Semenogelin II, SGII, SEMG2

3 Images
Sandwich ELISA - Human SEMG2 ELISA Kit (AB240682)
  • sELISA

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Sandwich ELISA - Human SEMG2 ELISA Kit (AB240682)

Interpolated concentrations of spiked SEMG2 in human serum and female urine samples. The concentrations of SEMG2 were measured in duplicates, interpolated from the SEMG2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, and female urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human SEMG2 ELISA Kit (AB240682)
  • sELISA

Unknown

Sandwich ELISA - Human SEMG2 ELISA Kit (AB240682)

Interpolated concentrations of native SEMG2 in human male urine and female urine samples.

The concentrations of SEMG2 were measured in duplicates, interpolated from the SEMG2 standard curves and corrected for sample dilution. Undiluted samples are as follows : male urine 1 : 17 and female urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SEMG2 concentration was determined to be 79.2 μg/mL in male urine and was undetectable in female urine.

Sandwich ELISA - Human SEMG2 ELISA Kit (AB240682)
  • sELISA

Unknown

Sandwich ELISA - Human SEMG2 ELISA Kit (AB240682)

Example of human SEMG2 standard curve in Sample Diluent NS. The SEMG2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 8.4 ng/mL

Range

9.38 - 600 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human SEMG2 ELISA Kit (ab240682) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SEMG2 protein in serum and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human SEMG2 with 8.4 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Urine", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.2" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": "= 96" }, { "sample": "Urine", "range": null, "average": "= 81" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab240682-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB240682-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB240682-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB240682-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB240682-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB240682-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB240682-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human SEMG2 Capture Antibody", "number":"AB240682-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human SEMG2 Lyophilized recombinant Protein", "number":"AB240682-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB240682-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"10X Human SEMG2 Detector Antibody", "number":"AB240682-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Semenogelin II also known as SGII is a protein that plays a significant role in human reproduction. It is a major component of semen and weighs approximately 50 kDa. This protein is primarily expressed in the seminal vesicles. SGII is closely related to Semenogelin I (SGI) and shares a high degree of sequence homology. The presence of these proteins is important to semen coagulation affecting viscosity and ultimately fertility.
Biological function summary

Semenogelin II contributes to the formation of the seminal coagulum which provides an essential function in immobilizing sperm temporarily after ejaculation. This protein interacts within a complex network involving prostate-specific antigen (PSA) which partially degrades SGII to liquefy the coagulum allowing sperm motility and facilitating fertilization. The activity of SGII in semen coagulation and subsequent liquefaction demonstrates its impact on reproductive success.

Pathways

Semenogelin II is integral to the reproductive pathway involving sperm maturation and fertilization. The effort of PSA to degrade SGII and its counterpart Semenogelin I is central to the regulation of semen liquefaction. This interaction is important to the seminal plasma proteolysis pathway. Proteins such as matrix metalloproteinases are also part of this pathway although they have a distinct and less direct relationship with SGII.

Abnormalities in the function or expression of Semenogelin II have implications for infertility. Altered SGII levels or dysfunction can impact semen coagulation which may lead to reduced sperm motility and decreased chances of successful fertilization. Additionally SGII is studied for its potential involvement in prostate cancer since its regulation by PSA suggests a connection. Understanding SGII's role in these contexts might provide insights into both reproductive health and prostate disease management.
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Product protocols

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Target data

Participates in the formation of a gel matrix (sperm coagulum) entrapping the accessory gland secretions and ejaculated spermatozoa.
See full target information Semenogelin-2
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websiteProtocolBooklet
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