Human SEPP1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human SEPP1 with a sensitivity of 12.65 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Heparin Plasma, Citrate plasma, EDTA Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 31.25 - 2000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 12.65 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Might be responsible for some of the extracellular antioxidant defense properties of selenium or might be involved in the transport of selenium. May supply selenium to tissues such as brain and testis.
Alternative names
SELP, SEPP1, SELENOP, Selenoprotein P, SeP
What's included?
Recommended products
Human SEPP1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human SEPP1 with a sensitivity of 12.65 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Heparin Plasma, Citrate plasma, EDTA Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 31.25 - 2000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 12.65 pg/mL
- General recovery
- 98%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 10.9 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 1.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 86 | Range 81 - 88 % |
Sample type Citrate plasma | Average % = 109 | Range 98 - 124 % |
Sample type EDTA Plasma | Average % = 117 | Range 110 - 122 % |
Sample type Heparin Plasma | Average % = 107 | Range 101 - 112 % |
Sample type Serum | Average % = 98 | Range 91 - 107 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human SEPP1 SimpleStep ELISA® kit (ab303751) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human SEPP1 protein in serum, plasma (hep), plasma (cit), plasma (EDTA) and cell culture supernatant. Quantitate Human SEPP1 with 12.65 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SEPP1 also known as Selenoprotein P is a glycoprotein characterized by multiple selenocysteine residues which play a critical role in its function. SEPP1 has a molecular mass of approximately 60 kDa. It expresses predominantly in the liver but can be found in other tissues like the brain kidney and plasma. In the liver it is involved in selenium transport and homeostasis carrying selenium to other tissues through the blood.
- Biological function summary
SEPP1 acts as an antioxidant helping to defend cells against damage from reactive oxygen species. It exists as part of a complex system within the body to balance oxidative stress. Moreover SEPP1 contributes to the recycling of selenium in the body ensuring adequate supply for selenoprotein synthesis in various tissues. It serves essential functions in neurological health and immune response leveraging its selenium content to protect cells and maintain cellular activities.
- Pathways
SEPP1 is significantly involved in selenium homeostasis and the oxidative stress response pathways. It interacts with proteins like glutathione peroxidase (GPx) and thioredoxin reductase which also depend on selenium for their antioxidant activities. The involvement of SEPP1 in these pathways is critical for mitigating oxidative damage and supporting the function of other selenium-dependent enzymes essential for maintaining cellular health.
- Associated diseases and disorders
SEPP1 is associated with neurodegenerative diseases and certain types of cancer. Dysregulation of SEPP1 and selenium homeostasis may contribute to the development and progression of conditions such as Alzheimer's disease potentially involving interactions with amyloid precursor protein (APP) and tau proteins. In cancer alterations in SEPP1 expression have been linked to colorectal cancer through pathways intersecting with proteins influencing cell proliferation and apoptosis such as Bcl-2 and p53 indicating its potential role in tumor biology.
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4 product images
Sandwich ELISA - Human SEPP1 ELISA Kit (ab303751)
Example of human SEPP1 standard curve in Sample Diluent NS.
Example of human SEPP1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human SEPP1 ELISA Kit (ab303751)
Interpolated concentrations of human SEPP1 in HepG2 supernatant.
Interpolated concentration of native SEPP1 was measured in duplicate at different sample concentrations. Undiluted samples are 2.5% HepG2 supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human SEPP1 ELISA Kit (ab303751)
Interpolated concentrations of human SEPP1 in serum, plasma (citrate), plasma (heparin), and plasma (EDTA).
Interpolated concentration of native SEPP1 was measured in duplicate at different sample concentrations. Undiluted samples are 1: 10,000 serum, plasma (citrate), plasma (heparin), and plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human SEPP1 ELISA Kit (ab303751)
Interpolated concentrations of SEPP1 in normal human serum donors.
Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean SEPP1 concentration was determined to be 10.28 μg/mL with a range of 1.41 – 14.21 μg/mL.
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