AB303751

Human SEPP1 ELISA Kit

Recombinant
SimpleStep
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Human SEPP1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human SEPP1 with a sensitivity of 12.65 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

SELP, SEPP1, SELENOP, Selenoprotein P, SeP

4 Images

Sandwich ELISA - Human SEPP1 ELISA Kit (AB303751)

sELISA

Supplier Data

Sandwich ELISA - Human SEPP1 ELISA Kit (AB303751)

Interpolated concentrations of human SEPP1 in HepG2 supernatant.

Interpolated concentration of native SEPP1 was measured in duplicate at different sample concentrations. Undiluted samples are 2.5% HepG2 supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human SEPP1 ELISA Kit (AB303751)

Interpolated concentrations of SEPP1 in normal human serum donors.

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean SEPP1 concentration was determined to be 10.28 μg/mL with a range of 1.41 – 14.21 μg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human SEPP1 ELISA Kit (AB303751)

Interpolated concentrations of human SEPP1 in serum, plasma (citrate), plasma (heparin), and plasma (EDTA).

Interpolated concentration of native SEPP1 was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 10,000 serum, plasma (citrate), plasma (heparin), and plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human SEPP1 ELISA Kit (AB303751)

Example of human SEPP1 standard curve in Sample Diluent NS.

Example of human SEPP1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Heparin Plasma, Citrate plasma, EDTA Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 12.65 pg/mL

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human SEPP1 SimpleStep ELISA® kit (ab303751) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human SEPP1 protein in serum, plasma (hep), plasma (cit), plasma (EDTA) and cell culture supernatant. Quantitate Human SEPP1 with 12.65 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.9" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "81 - 88 %", "average": "= 86" }, { "sample": "Citrate plasma", "range": "98 - 124 %", "average": "= 109" }, { "sample": "EDTA Plasma", "range": "110 - 122 %", "average": "= 117" }, { "sample": "Heparin Plasma", "range": "101 - 112 %", "average": "= 107" }, { "sample": "Serum", "range": "91 - 107 %", "average": "= 98" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SEPP1 also known as Selenoprotein P is a glycoprotein characterized by multiple selenocysteine residues which play a critical role in its function. SEPP1 has a molecular mass of approximately 60 kDa. It expresses predominantly in the liver but can be found in other tissues like the brain kidney and plasma. In the liver it is involved in selenium transport and homeostasis carrying selenium to other tissues through the blood.

Biological function summary

SEPP1 acts as an antioxidant helping to defend cells against damage from reactive oxygen species. It exists as part of a complex system within the body to balance oxidative stress. Moreover SEPP1 contributes to the recycling of selenium in the body ensuring adequate supply for selenoprotein synthesis in various tissues. It serves essential functions in neurological health and immune response leveraging its selenium content to protect cells and maintain cellular activities.

Pathways

SEPP1 is significantly involved in selenium homeostasis and the oxidative stress response pathways. It interacts with proteins like glutathione peroxidase (GPx) and thioredoxin reductase which also depend on selenium for their antioxidant activities. The involvement of SEPP1 in these pathways is critical for mitigating oxidative damage and supporting the function of other selenium-dependent enzymes essential for maintaining cellular health.

SEPP1 is associated with neurodegenerative diseases and certain types of cancer. Dysregulation of SEPP1 and selenium homeostasis may contribute to the development and progression of conditions such as Alzheimer's disease potentially involving interactions with amyloid precursor protein (APP) and tau proteins. In cancer alterations in SEPP1 expression have been linked to colorectal cancer through pathways intersecting with proteins influencing cell proliferation and apoptosis such as Bcl-2 and p53 indicating its potential role in tumor biology.

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Product protocols

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Target data

Might be responsible for some of the extracellular antioxidant defense properties of selenium or might be involved in the transport of selenium. May supply selenium to tissues such as brain and testis.

See full target information SELENOP

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websiteProtocolBooklet

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human SEPP1 ELISA Kit

Sandwich ELISA - Human SEPP1 ELISA Kit (AB303751)

Sandwich ELISA - Human SEPP1 ELISA Kit (AB303751)

Sandwich ELISA - Human SEPP1 ELISA Kit (AB303751)

Sandwich ELISA - Human SEPP1 ELISA Kit (AB303751)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Assay Kits

Product promise